Contact us at 1.800.501.7654 or info@biossusa.com

MenuSearch
Help

MCM2 (S41) Recombinant Antibody

25 ug total protein per lane of various lysates (see on figure) probed with phospho-MCM2 (S41) monoclonal antibody, unconjugated (bsm-63313R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with phospho-MCM2 (S41) monoclonal antibody, unconjugated (bsm-63313R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-MCM2 (S41) Monoclonal Antibody, Unconjugated (bsm-63313R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-MCM2 (S41) Monoclonal Antibody, Unconjugated (bsm-63313R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-MCM2 (S41) Monoclonal Antibody, Unconjugated (bsm-63313R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-MCM2 (S41) Monoclonal Antibody, Unconjugated (bsm-63313R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-MCM2 (S41) Monoclonal Antibody, Unconjugated (bsm-63313R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-MCM2 (S41) Monoclonal Antibody, Unconjugated (bsm-63313R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-MCM2 (S41) Monoclonal Antibody, Unconjugated (bsm-63313R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-MCM2 (S41) Monoclonal Antibody, Unconjugated (bsm-63313R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-MCM2 (S41) Monoclonal Antibody, Unconjugated (bsm-63313R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-MCM2 (S41) Monoclonal Antibody, Unconjugated (bsm-63313R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-MCM2 (S41) Monoclonal Antibody, Unconjugated (bsm-63313R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-MCM2 (S41) Monoclonal Antibody, Unconjugated (bsm-63313R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-63313r-100uL
Product Name MCM2 (S41) Recombinant Antibody
Applications
WB IHC-P IHC-F IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source A synthesized peptide derived from human MCM2 around the phosphorylation site of S41
Clonality Recombinant
Clone # 5G4
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 4171
Swiss Prot P49736
Subcellular location Nucleus
Synonyms MCM2 (phospho-S41); p-MCM2; phospho-MCM2; MCM2 (phospho-Ser41); BM28; CCNL1; CDCL1; D3S3194; DFNA70; MITOTIN; cdc19; Mcmd2; mKIAA0030; MCM2_HUMAN; MCM2; Minichromosome maintenance protein 2 homolog; Nuclear protein BM28; 3.6.4.12; KIAA0030; MCM2_MOUSE;
Application Dilution
WB =1:500-2000
IHC-P IHC-P=1:100-200
IHC-F IHC-F=1:100-200
IF IF=1:100-200

Frequently asked questions

Which applications is MCM2 (S41) Recombinant Antibody (bsm-63313R) validated for, and at what dilution?

bsm-63313R is validated for WB (WB=1:500-2000); IHC-P (IHC-P=1:100-200); IHC-F (IHC-F=1:100-200); IF (IF=1:100-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does MCM2 (S41) Recombinant Antibody (bsm-63313R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-63313R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

What immunogen was used to raise MCM2 (S41) Recombinant Antibody (bsm-63313R)?

A synthesized peptide derived from human MCM2 around the phosphorylation site of S41

Search our catalog of hundreds of thousands of products

Free sample offer

Try a recombinant sample

20 µL sample

Freewith your paid order

View product details (opens in a new tab)

View cart

US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

Primary antibody added to your cart

Need a secondary antibody?

Product validation image

View product details and validation

Confirm application and cross-reactivity before use. Prices shown are before eligible cart discounts. Shipping and tax are calculated at checkout.

View cart