Alpha II Spectrin Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-70005M-100uL |
| Product Name | Alpha II Spectrin Antibody |
| Applications |
WB
IHC
|
| Specificity | Specific for endogenous levels of the ~240 kDa alpha II spectrin protein. |
| Reactivity | Human, Mouse, Rat, Bovine |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Recombinant contstruct containing the 7th, 8th, 9th repeats of human alpha II spectrin. |
| Clonality | Monoclonal |
| Clone # | 3D7 |
| Isotype | IgG1 |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | PBS + 50% glycerol and 5 mM NaN3 |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Gene ID | 6709 |
| Swiss Prot | Q13813 |
| Synonyms | (ALPHA)II-SPECTRIN antibody, Alpha-II spectrin antibody, brain antibody, EIEE5 antibody, 7738 antibody, FLJ44613 antibody, Fodrin antibody, Fodrin alpha chain antibody, Fodrin, alpha antibody, NEAS antibody, Non erythrocytic spectrin alpha antibody, non-erythroid alpha chain antibody, SPECA antibody, Spectrin alpha chain antibody, Spectrin alpha chain brain antibody, Spectrin alpha non erythrocytic 1 antibody, Spectrin antibody, Spectrin non erythroid alpha chain antibody, Spectrin, alpha, non-erythrocytic 1 (alpha-fodrin) antibody, Spectrin, nonerythroid, alpha subunit antibody, Spna2 antibody, SPTA 2 antibody, SPTA2 antibody, SPTA2_HUMAN antibody, SPTAN 1 antibody, SPTAN1 antibody |
| Background | The spectrin family of cytoskeletal proteins is comprised of 2 alpha genes (α1 and α2) and five beta genes (β1-β5). Spectrins have been shown to function as scaffolding proteins in mechanical support of the plasma membrane as well as bind other membrane proteins and lipids (Bennett and Baines 2001). Defects in spectrin genes have been linked to some forms of hereditary spherocytosis, a type of auto-hemolytic anemia which is characterized by spherical red blood cells that are more prone to lysis (Eber and Lux 2004). |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC | 1:50-200 |
Frequently asked questions
Which applications is Alpha II Spectrin Antibody (bsm-70005M) validated for, and at what dilution?
bsm-70005M is validated for WB (1:300-5000); IHC (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Alpha II Spectrin Antibody (bsm-70005M) react with?
Tested reactivity: Human, Mouse, Rat, Bovine.
How should bsm-70005M be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: PBS + 50% glycerol and 5 mM NaN3
What immunogen was used to raise Alpha II Spectrin Antibody (bsm-70005M)?
Recombinant contstruct containing the 7th, 8th, 9th repeats of human alpha II spectrin.