Alpha Actinin 4 Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-70007M-100uL |
| Product Name | Alpha Actinin 4 Antibody |
| Applications |
WB
IHC
|
| Specificity | Specific for endogenous levels of the ~105 kDa α-actinin 4 protein. |
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Fusion protein from the central rod domain of human α-actinin 4. |
| Clonality | Monoclonal |
| Clone # | 93 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | O43707 |
| Synonyms | actinin 4 antibody, Actinin alpha 4 antibody, actinin4 antibody, ACTN 4 antibody, ACTN4 antibody, ACTN4_HUMAN antibody, alpha Actinin 4 antibody, Alpha-actinin-4 antibody, DKFZp686K23158 antibody, F actin cross linking protein antibody, F-actin cross-linking protein antibody, Focal segmental glomerulosclerosis 1 antibody, FSGS 1 antibody, FSGS antibody, FSGS1 antibody, Non muscle alpha actinin 4 antibody, Non-muscle alpha-actinin 4 antibody |
| Background | α-actinin-4 is a member of the actinin protein family comprised of an actin-binding domain in the N-terminus, 4 spectrin-like repeats in the central region, and 2 EF-hand motifs in the C-terminus (Honda et al, 1998). α-actinin-4 and CLP36 form a complex in normal kidney podocytes.CLP36 is dependent on α-actinin-4 for maintenance of its level in podocytes, whereas α-actinin-4 is independent of CLP36. α-actinin-4 is widely expressed in mammalian tissues and organs, while having a high occurrence of genetic mutations in kidney podocytes (Kos et al, 2003). FSGS, focal segmental glomerulosclerosis, is a rare genetic disease that attacks the kidney's filtering units (glomeruli) causing serious scarring which leads to permanent kidney damage and even failure. Three key mutations have been found in α-actinin-4 in people diagnosed with FSGS. R310Q and Q348R, located in the spectrin-like repeats region, and K255E located in the actin-binding region. The R310Q and Q348R mutation significantly inhibits the ability of α-actinin-4 to form the complex with CLP36. The K255E mutation was reversed where it increased the ability to bind CLP36 in the actin-binding region (Liu et al, 2011). |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC | 1:50-200 |
Frequently asked questions
Which applications is Alpha Actinin 4 Antibody (bsm-70007M) validated for, and at what dilution?
bsm-70007M is validated for WB (1:300-5000); IHC (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Alpha Actinin 4 Antibody (bsm-70007M) react with?
Tested reactivity: Human, Mouse.
How should bsm-70007M be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
What immunogen was used to raise Alpha Actinin 4 Antibody (bsm-70007M)?
Fusion protein from the central rod domain of human α-actinin 4.