CD75 (Transferrin receptor) Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-70034M-100uL |
| Product Name | CD75 (Transferrin receptor) Antibody |
| Applications |
WB
IHC
|
| Specificity | Specific for endogenous levels of the ~35 kDa CD75 protein. |
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Nuclei isolated from pokeweed mitogen-stimulated human peripheral blood lymphocytes. |
| Clonality | Monoclonal |
| Clone # | LN1 |
| Isotype | IgM |
| Concentration | Lot Dependent |
| Purification | Protein L purified |
| Storage Buffer | PBS |
| Storage Condition | Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cy |
| Target | |
| Gene ID | 6480 |
| Swiss Prot | P15907 |
| Synonyms | 6 sialyltransferase antibody, 6-sialyltransferase 1 antibody, 6-ST 1 antibody, Alpha 2 antibody, Alpha 2,6 ST 1 antibody, Alpha 2,6 ST antibody, B cell antigen CD75 antibody, B-cell antigen CD75 antibody, Beta galactoside alpha 2,6 sialyltransferase 1 antibody, Beta-galactoside alpha-2 antibody, CMP N acetylneuraminate beta galactosamide alpha 2,6 sialyltransferase 1 antibody, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2 antibody, MGC48859 antibody, Sialyltransferase 1 (beta galactoside alpha 2,6 sialyltransferase) antibody, Sialyltransferase 1 antibody, SIAT1 antibody, SIAT1_HUMAN antibody, ST6 beta galactosamide alpha 2,6 sialyltranferase 1 antibody, ST6Gal I antibody, ST6GAL1 antibody, ST6GalI antibody, ST6N antibody |
| Background | CD75, also known as β-galactoside α2,6-sialyltransferase, is a specific carbohydrate antigen that is predominantly expressed on B cell lymphocytes. β-galactoside α2,6-sialyltransferase is an enzyme that catalyzes the addition of sialic acid to growing carbohydrate chains of glycoproteins (Guy, K., et al, 1991). CD75 is a major component of the lymphoid cell surface that serves as a ligand for cell adhesion molecules (Paulson, J.C., et al, 1989). The LN-1 clone reacts positively with red blood cell precursors of the bone marrow, ciliated epithelial cells of the bronchus, distal tubular cells of the kidney, and ductal cells from several organs including the breast and prostate (Epstein, A.L., et al, 1984). CD75 has been shown to be superior to other B cell markers in detecting lymphocyte predominant cells through their cytoplasmic and membraneous staining, especially in typical nodular lymphocyte-predominant Hodgkin lymphoma patterns (Carbone, A., et al, 2014). |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC | 1:50-200 |
Frequently asked questions
Which applications is CD75 (Transferrin receptor) Antibody (bsm-70034M) validated for, and at what dilution?
bsm-70034M is validated for WB (1:300-5000); IHC (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD75 (Transferrin receptor) Antibody (bsm-70034M) react with?
Tested reactivity: Human.
How should bsm-70034M be stored?
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cy Storage buffer: PBS
What immunogen was used to raise CD75 (Transferrin receptor) Antibody (bsm-70034M)?
Nuclei isolated from pokeweed mitogen-stimulated human peripheral blood lymphocytes.