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Hsp60 Antibody

Applications

  • WB
  • IHC

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Bovine
  • Sheep
  • Horse
  • Cat
  • Rabbit
  • Guinea Pig
  • Goat
  • Zebrafish
  • Hamster
  • Non-Human Primate
Overview
Catalog # bsm-70131M-100uL
Product Name Hsp60 Antibody
Applications
WB IHC
Specificity Specific for endogenous levels of the ~ 60 kDa HSP60 protein.
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Bovine, Sheep, Horse, Cat, Rabbit, Guinea Pig, Goat, Zebrafish, Hamster, Non-Human Primate
Specifications
Conjugation Unconjugated
Host Mouse
Source Recombinant full length human HSP60 protein.
Clonality Monoclonal
Clone # 1C7
Isotype IgG1
Concentration Lot Dependent
Purification Purified by Protein G.
Storage Buffer PBS + 50% glycerol and 5 mM NaN3
Storage Condition Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Gene ID 3329
Swiss Prot P10809
Synonyms 60 kDa chaperonin antibody, 60 kDa heat shock protein, mitochondrial antibody, CH60_HUMAN antibody, Chaperonin 60 antibody, Chaperonin, 60-KD antibody, CPN60 antibody, fa04a05 antibody, GROEL antibody, heat shock 60kDa protein 1 (chaperonin) antibody, Heat shock protein 1 (chaperonin) antibody, Heat shock protein 60 antibody, Heat shock protein 65 antibody, heat shock protein family D (Hsp60) member 1 antibody, HLD4 antibody, Hsp 60 antibody, HSP 65 antibody, HSP-60 antibody, HSP60 antibody, HSP65 antibody, HSPD1 antibody, HuCHA60 antibody, Mitochondrial matrix protein P1 antibody, P60 lymphocyte protein antibody, short heat shock protein 60 Hsp60s1 antibody, SPG13 antibody
Background HSP60 is a member of the heat shock protein family which is thought to play a key role in mitochondrial protein transport and proper refolding of proteins imported from the cytoplasm into the mitochondrial matrix (Koll et al., 1992). More recently, HSP60 has been implicated in the pathogenesis of cardiovascular disease (Rizzo et al., 2011).
Application Dilution
WB 1:300-5000
IHC 1:50-200

Frequently asked questions

Which applications is Hsp60 Antibody (bsm-70131M) validated for, and at what dilution?

bsm-70131M is validated for WB (1:300-5000); IHC (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Hsp60 Antibody (bsm-70131M) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Bovine, Sheep, Horse, Cat, Rabbit, Guinea Pig, Goat, Zebrafish, Hamster, Non-Human Primate.

How should bsm-70131M be stored?

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: PBS + 50% glycerol and 5 mM NaN3

What immunogen was used to raise Hsp60 Antibody (bsm-70131M)?

Recombinant full length human HSP60 protein.

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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