MeCP2 Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-70147M-100uL |
| Product Name | MeCP2 Antibody |
| Applications |
WB
IHC
|
| Specificity | Specific for endogenous levels of the MeCP2 protein. Due to usually high charge, the protein runs at ~75 kDa in SDS-PAGE of mouse brain lysates. |
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Full length human recombinant MeCP2 |
| Clonality | Monoclonal |
| Clone # | 5H12 |
| Isotype | IgG2b |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | PBS + 50% glycerol and 5 mM NaN3 |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Gene ID | 4204 |
| Swiss Prot | P51608 |
| Synonyms | AUTSX 3 antibody, AUTSX3 antibody, DKFZp686A24160 antibody, Mbd 5 antibody, Mbd5 antibody, MECP 2 antibody, MeCP 2 protein antibody, MeCP-2 protein antibody, Mecp2 antibody, MECP2_HUMAN antibody, Methyl CpG binding protein 2 (Rett syndrome) antibody, Methyl CpG binding protein 2 antibody, Methyl-CpG-binding protein 2 antibody, MRX 16 antibody, MRX 79 antibody, MRX16 antibody, MRX79 antibody, MRXS 13 antibody, MRXS13 antibody, MRXSL antibody, PPMX antibody, RS antibody, RTS antibody, RTT antibody, WBP 10 antibody, WBP10 antibody |
| Background | MeCP2 (Methyl-CpG Binding Protein 2) is a chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair and is not influenced by sequences flanking the methyl-CpGs. MeCP2 has been shown to mediate transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A (Nan et al., 1998). Defects in MeCP2 are the cause of Rett syndrome (RTT) (Amir et al., 1999). RTT is an X-linked dominant disease; it is a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC | 1:50-200 |
Frequently asked questions
Which applications is MeCP2 Antibody (bsm-70147M) validated for, and at what dilution?
bsm-70147M is validated for WB (1:300-5000); IHC (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MeCP2 Antibody (bsm-70147M) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-70147M be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: PBS + 50% glycerol and 5 mM NaN3
What immunogen was used to raise MeCP2 Antibody (bsm-70147M)?
Full length human recombinant MeCP2