Synaptobrevin (VAMP) Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-70271M-100uL |
| Product Name | Synaptobrevin (VAMP) Antibody |
| Applications |
WB
IHC
|
| Specificity | Specific for endogenous levels of the ~16 kDa synaptobrevin. The antibody has also been demonstrated to work in immunohistochemistry on formalin fixed, vibratome sections, but does not work on paraffin sections. |
| Reactivity | Human, Mouse, Rat, Bovine, Pig, Rabbit, Hamster |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Synaptic immunoprecipitate (crude) from human brain. |
| Clonality | Monoclonal |
| Clone # | SP10 |
| Isotype | IgM |
| Concentration | Lot Dependent |
| Purification | IgM Purified culture supernatant |
| Storage Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Gene ID | 6843 |
| Swiss Prot | P23763 |
| Synonyms | SYB antibody, SYB1 antibody, Synaptobrevin 1 antibody, VAMP antibody, VAMP1 antibody, Vesicle associated membrane protein 1 antibody |
| Background | Synaptobrevin (aka VAMP) is an integral membrane protein of synaptic vesicles that plays a major role in the formation of larger SNARE complexes, along with SNAP-25 and syntaxin. Synaptobrevin has been shown to be essential for two fast synapse-specific membrane trafficking processes: fast exocytosis for neurotransmitter release and fast endocytosis that mediates rapid recycling of synaptic vesicles (Deak et al., 2004). Decreased levels of synaptobrevin in human hippocampus and cortex have been correlated with cognitive deficits in Alzheimer’s disease (Sze et al., 2000). |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC | 1:50-200 |
Frequently asked questions
Which applications is Synaptobrevin (VAMP) Antibody (bsm-70271M) validated for, and at what dilution?
bsm-70271M is validated for WB (1:300-5000); IHC (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Synaptobrevin (VAMP) Antibody (bsm-70271M) react with?
Tested reactivity: Human, Mouse, Rat, Bovine, Pig, Rabbit, Hamster.
How should bsm-70271M be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
What immunogen was used to raise Synaptobrevin (VAMP) Antibody (bsm-70271M)?
Synaptic immunoprecipitate (crude) from human brain.