Vimentin Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-70312M-100uL |
| Product Name | Vimentin Antibody |
| Applications |
WB
IHC
|
| Specificity | Specific for endogenous levels of the ~50 kDa vimentin protein. |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Bovine, Sheep, Horse, Chicken, Cat, Rabbit, Guinea Pig, Goat, Zebrafish, Fish, Hamster, Drosophila, Xenopus, Non-Human Primate, Lizard, Vole, Finch |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Recombinant human vimentin purified from E. coli. |
| Clonality | Monoclonal |
| Clone # | 2D1 |
| Isotype | IgG2a |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | PBS + 10 mM NaN3. |
| Storage Condition | Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cy |
| Target | |
| Gene ID | 7431 |
| Swiss Prot | P08670 |
| Synonyms | CTRCT30 antibody, Epididymis luminal protein 113 antibody, FLJ36605 antibody, HEL113 antibody, VIM antibody, VIME_HUMAN antibody, Vimentin antibody |
| Background | Vimentin is the major protein subunit of the 10nm or intermediate filaments (IFs) found in many kinds of mesenchymal and epithelial cells as well as developing neuronal and astrocytic precursor cells in the CNS. Vimentin is thought to be critically involved in lymphocyte adhesion and transmigration (Nieminen M et al. 2006). Copolymers are frequently formed between vimentin and other IFs, such as GFAP (in many kinds of astrocytes), desmin (in muscle cells) and neurofilament proteins (in developing neurons). Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton. Recent studies suggest that vimentin affects prostate cancer cells motility and invasiveness (Zhao et al. 2008). |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC | 1:50-200 |
Frequently asked questions
Which applications is Vimentin Antibody (bsm-70312M) validated for, and at what dilution?
bsm-70312M is validated for WB (1:300-5000); IHC (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Vimentin Antibody (bsm-70312M) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Bovine, Sheep, Horse, Chicken, Cat, Rabbit, Guinea Pig, Goat, Zebrafish, Fish, Hamster, Drosophila, Xenopus, Non-Human Primate, Lizard, Vole, Finch.
How should bsm-70312M be stored?
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cy Storage buffer: PBS + 10 mM NaN3.
What immunogen was used to raise Vimentin Antibody (bsm-70312M)?
Recombinant human vimentin purified from E. coli.