APC (C-terminal region) Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-70338t-100ul |
| Product Name | APC (C-terminal region) Antibody |
| Applications |
WB
|
| Specificity | This antibody detects a primarily full length APC at 300 kDa* in human Hct116 cells, mouse brain, and in rat PC12 cells. In immunocytochemistry, the antibody detects in APC in clusters at the plasma membrane and at the ends of microtubules. This sequence is highly conserved in human and rat APC, and has low homology to APC2. |
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rat |
| Source | Clone KT45 was generated from a recombinant protein containing amino acid residues from the C-terminal region of mouse APC. |
| Clonality | Monoclonal |
| Clone # | KT45 |
| Isotype | IgG2a |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | PBS + 0.05% NaN3 |
| Storage Condition | Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cy |
| Target | |
| Swiss Prot | Q61315 |
| Synonyms | adenomatous polyposis coli, mAPC, Deleted in polyposis, DP2.5, Microtubule |
| Background | The microtubule (MT) plus-end is a crucial site for the regulation of MT dynamics and MT association with organelles by several groups of plus-end tracking proteins (+TIPs). These +TIPs form comet-like accumulations at the plus ends of MTs to regulate MT dynamics and interactions. The +TIPs include diverse groups of proteins, such as motor and nonmotor proteins, MT polymerases and depolymerases as well as various regulatory and adaptor proteins. One group of +TIPs include proteins with basic and serine-rich motifs (SxIP motifs) that mediate interaction between MTs and EB proteins. Adenomatous polyposis coli (APC), MACF, and STM1 are a group of the SxIP motif-containing proteins. APC protein is a large multidomain tumor suppresor protein that has important roles in Wnt signaling, as well as several other cell functions including cell migration, spindle assembly, chromosome segregation, neuronal differentiation, apoptosis, and MT stabilization. APC interaction with EB proteins through its SxIP motif promotes interaction with MTs leading to stabilization and increased polymerization. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is APC (C-terminal region) Antibody (bsm-70338T) validated for, and at what dilution?
bsm-70338T is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does APC (C-terminal region) Antibody (bsm-70338T) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-70338T be stored?
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cy Storage buffer: PBS + 0.05% NaN3
What immunogen was used to raise APC (C-terminal region) Antibody (bsm-70338T)?
Clone KT45 was generated from a recombinant protein containing amino acid residues from the C-terminal region of mouse APC.