Axl/UFO (Extracellular region) Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-70355M-100uL |
| Product Name | Axl/UFO (Extracellular region) Antibody |
| Applications |
WB
IP
|
| Specificity | Clone M047 mouse monoclonal antibody detects a 140 kDa* protein on SDS-PAGE "Native" or denatured immunoblots of human A549, NCI-H1915, and MDA-MB-231 carcinomas. The antibody detects Axl in membranes and cytoplasm in MDA-MB-231 cell after immunocytochemical labeling. The antibody works for western blot, immunoprecipitation, immunocytochemistry, and ELISA capture. |
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Clone (M047) was generated from a recombinant protein that included the extracellular region of human Axl protein. |
| Clonality | Monoclonal |
| Clone # | M047 |
| Isotype | IgG1 |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | P30530 |
| Synonyms | Tyrosine-protein kinase receptor, UFO, Axl, Tyro7, ARK, |
| Background | The Axl/UFO receptor tyrosine kinase (RTKs) family includes Axl/UFO/Tyro7, Sky/Tyro3, and c-Mer/Tyro12. These RTKs have a conserved intracellular tyrosine kinase domain and extracellular domains that include immunoglobulin-like and fibronectin-type moieties similar to those found in cell adhesion molecules. The ligand for these receptors is the vitamin Kdependent protein growth-arrest-specific 6 (Gas6), which is structurally related to the protein S anticoagulation factor. Upon binding to its receptor, Gas6 activates phosphatidylinositol 3- kinase (PI3K) and its downstream targets Akt and S6K, as well as NF-κB. Axl is overexpressed in several cancers, including breast, lung, liver, colon, gastric, ovarian, pancreatic, and glioblastoma. The Axl/Gas6 signalling pathway has been shown to drive cancer cell survival, proliferation, migration and invasion, and several therapeutic strategies are being developed to regulate Axl cell signaling. |
| Application Dilution | |
| WB | 1:300-5000 |
| IP | 1-2ug |
Frequently asked questions
Which applications is Axl/UFO (Extracellular region) Antibody (bsm-70355M) validated for, and at what dilution?
bsm-70355M is validated for WB (1:300-5000); IP (1-2ug). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Axl/UFO (Extracellular region) Antibody (bsm-70355M) react with?
Tested reactivity: Human.
How should bsm-70355M be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
What immunogen was used to raise Axl/UFO (Extracellular region) Antibody (bsm-70355M)?
Clone (M047) was generated from a recombinant protein that included the extracellular region of human Axl protein.