E-Cadherin (Cytoplasmic) Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-70360M-100uL |
| Product Name | E-Cadherin (Cytoplasmic) Antibody |
| Applications |
WB
IP
IHC
|
| Specificity | This E-cadherin antibody detects a 120 kDa* protein in human A431 cells, and does not cross-react with VE-cadherin or N-cadherin. |
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Clone (M168) was generated from a mouse recombinant E-Cadherin protein containing amino acids in the cytoplasmic region. This sequence is highly conserved in human and rat E-cadherin. |
| Clonality | Monoclonal |
| Clone # | M168 |
| Isotype | IgG1 |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | P09803 |
| Synonyms | Uvomorulin, Cadherin-1, CTF1, CTF2, CTF3, CD324, Epithelial Cadherin |
| Background | Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. This region induces clustering and also binds to the protein p120 catenin. The cytoplasmic region is highly conserved in sequence and has been shown experimentally to regulate the cell-cell binding function of the extracellular domain of E-cadherin, possibly through interaction with the cytoskeleton. Many cadherins are regulated by phosphorylation, including N-cadherin and E-cadherin. N-cadherin is phosphorylated by c-Src at Tyr-820, Tyr-853, Tyr-860, Tyr-884, and Tyr-886. Phosphorylation of Tyr-860 can disrupt cadherin binding to β-catenin. Since many of these tyrosine sites are conserved in the cadherin family, phosphorylation of these sites may be critical for cadherin function. |
| Application Dilution | |
| WB | 1:300-5000 |
| IP | 1-2ug |
| IHC | 1:50-200 |
Frequently asked questions
Which applications is E-Cadherin (Cytoplasmic) Antibody (bsm-70360M) validated for, and at what dilution?
bsm-70360M is validated for WB (1:300-5000); IP (1-2ug); IHC (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does E-Cadherin (Cytoplasmic) Antibody (bsm-70360M) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-70360M be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
What immunogen was used to raise E-Cadherin (Cytoplasmic) Antibody (bsm-70360M)?
Clone (M168) was generated from a mouse recombinant E-Cadherin protein containing amino acids in the cytoplasmic region. This sequence is highly conserved in human and rat E-cadherin.