Caspase-3 (N-terminal region) Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-70373M-100uL |
| Product Name | Caspase-3 (N-terminal region) Antibody |
| Applications |
WB
|
| Specificity | The antibody detects a 32 kDa* protein corresponding to the molecular weight of Caspase-3 on SDS-PAGE immunoblots of human Jurkat and K562 cells. |
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Clone (M377) was generated from a recombinant protein containing amino acid residues in the N-terminal region of human Caspase-3. |
| Clonality | Monoclonal |
| Clone # | M377 |
| Isotype | IgG2a |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | P42574 |
| Synonyms | Caspase,-3, CPP-32, Apoptain, Yama, SCA-1, CASP-2, NEDD2, ICH-1, p18, p13, p12 |
| Background | The caspases are a group of cysteine enzymes, which cleave proteins in response to intrinsic and extrinsic pathways that cause apoptotic cell death. The caspases can be grouped into two subgroups based on their roles in apoptosis. Initiator caspases (caspases 2, 8, 9, and 10) are activated through the apoptosis-signaling pathways and activate the effector caspases (caspases 3, 6, and 7) which carry out apoptosis. Caspase cascades are initiated through assembly of multiprotein complexes that trigger activation of the initiator caspases, which are then released and are able to activate the downstream effector caspases. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Caspase-3 (N-terminal region) Antibody (bsm-70373M) validated for, and at what dilution?
bsm-70373M is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Caspase-3 (N-terminal region) Antibody (bsm-70373M) react with?
Tested reactivity: Human.
How should bsm-70373M be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
What immunogen was used to raise Caspase-3 (N-terminal region) Antibody (bsm-70373M)?
Clone (M377) was generated from a recombinant protein containing amino acid residues in the N-terminal region of human Caspase-3.