β-Catenin Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-70379m-100ul |
| Product Name | β-Catenin Antibody |
| Applications |
WB
IP
IHC
|
| Specificity | The antibody detects a 92 kDa* protein corresponding to the molecular mass of β-Catenin on SDS-PAGE immunoblots of A431 and Hct116 src transformed cells. In addition, this antibody recognizes only β-Catenin in immunoprecipitations using anti-β-Catenin versus γ-Catenin. This peptide sequence is highly conserved in rat and mouse β-Catenin. |
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Clone (M118) was generated from a recombinant protein corresponding to amino acid residues in the C-terminal region of human β-Catenin. |
| Clonality | Monoclonal |
| Clone # | M118 |
| Isotype | IgG1 |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | P35222 |
| Synonyms | Catenin beta1, CTNNB1, catenin |
| Background | β-Catenin is a 92 kDa protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions. Deletions in the cytoplasmic domain of E-Cadherin eliminate catenin binding and result in a loss of cell adhesion. Tyrosine phosphorylation of β-Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn kinases phosphorylate tyrosine 142 in vitro. Overexpression of these kinases in epithelial cells disrupts interactions between α- and β-Catenins. The phosphorylation of tyrosine 142 may act as a switch from the transcriptional to the adhesive role of β-Catenin. Src family kinases can also phosphorylate tyrosine 86 and 654 in β-Catenin. The Tyr-654 phosphorylation regulates β-Catenin binding to E-cadherin. Thus, site-specific tyrosine phosphorylation of β-Catenin may regulate protein-protein interactions leading to changes in cell adhesion. |
| Application Dilution | |
| WB | 1:300-5000 |
| IP | 1-2ug |
| IHC | 1:50-200 |
Frequently asked questions
Which applications is β-Catenin Antibody (bsm-70379M) validated for, and at what dilution?
bsm-70379M is validated for WB (1:300-5000); IP (1-2ug); IHC (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does β-Catenin Antibody (bsm-70379M) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-70379M be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
What immunogen was used to raise β-Catenin Antibody (bsm-70379M)?
Clone (M118) was generated from a recombinant protein corresponding to amino acid residues in the C-terminal region of human β-Catenin.