c-Cbl (Tyr-700), Phosphospecific Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-70394m-100ul |
| Product Name | c-Cbl (Tyr-700), Phosphospecific Antibody |
| Applications |
WB
|
| Specificity | The antibody detects a 120 kDa* protein in human Jurkat cells treated with pervanadate, but not in control cells. This sequence is highly conserved in rat and mouse c-Cbl. |
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Clone (M161) was generated from a synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding tyrosine 700 in human c-Cbl. |
| Modification Site | Tyr-700 |
| Clonality | Monoclonal |
| Clone # | M161 |
| Isotype | IgG1 |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | P22681 |
| Synonyms | E3 ubiquitin ligase CBL, RING finger 55 |
| Background | c-Cbl proto-oncogene is a ubiquitously expressed cytoplasmic adaptor protein that is especially predominant in hematopoietic cells. c-Cbl possesses a highly conserved amino-terminal phosphotyrosine binding domain and a C3HC4 RING finger motif. c-Cbl is rapidly tyrosine phosphorylated in response to stimulation of a variety of cell-surface receptors and becomes associated with a number of intracellular signaling molecules such as protein tyrosine kinases, phosphatidylinositol 3 kinase (PI3K), Crk and 14-3-3 proteins. In human cancer tissues, c-Cbl is frequently tyrosine phosphorylated in a tumor-specific manner. Src-family kinases primarily phosphorylate c-Cbl at Tyr-700, Tyr-731, and Tyr-774. These sites of phosphorylation provide docking sites for downstream signaling components, such as Grb-2, PI3K, and Fyn. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is c-Cbl (Tyr-700), Phosphospecific Antibody (bsm-70394M) validated for, and at what dilution?
bsm-70394M is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does c-Cbl (Tyr-700), Phosphospecific Antibody (bsm-70394M) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-70394M be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
What immunogen was used to raise c-Cbl (Tyr-700), Phosphospecific Antibody (bsm-70394M)?
Clone (M161) was generated from a synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding tyrosine 700 in human c-Cbl.