CD46 (Extracellular region) Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-70400M-100uL |
| Product Name | CD46 (Extracellular region) Antibody |
| Applications |
WB
IP
|
| Specificity | Clone M037 mouse monoclonal antibody detects a 45-60 kDa* protein on SDS-PAGE "Native" immunoblots of human A549, MeWo, MDA-MB-231, and MCF7 cells, as well as human lung tissues. This antibody weakly detects denatured CD46. The antibody works for western blot, immunoprecipitation, ELISA capture, and immunocytochemistry. |
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Clone (M037) was generated from a proprietary antigen related to native human CD46 expressed in NCI-H1915 lung cancer cell line. |
| Clonality | Monoclonal |
| Clone # | M037 |
| Isotype | IgG2b |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | P15529 |
| Synonyms | Membrane cofactor protein, TLX, Trophoblast leukocyte common antigen, CD46, MCP, MIC10 |
| Background | CD46 is a complement regulatory protein that is also called membrane cofactor protein. This protein is a type 1 membrane protein that plays an important inhibitory role in the complement system. CD46 exhibits a cofactor activity that promotes inactivation of C3b and C4b by whole antiserum factor 1, thereby protecting host cells from complement-dependent cytotoxicity. CD46 can also function as a receptor for selected bacteria and viruses, and is reportedly required for proper fusion of spermatozoa to the oocyte membrane during fertilization. CD46 is overexpressed in medulloblastoma tumors, and CD46 expression has been linked with poor prognosis in breast cancer. The upregulation of CD46 may protect cancer cells from complement-dependent cytotoxicity to facilitate cancer cell immune evasion. |
| Application Dilution | |
| WB | 1:300-5000 |
| IP | 1-2ug |
Frequently asked questions
Which applications is CD46 (Extracellular region) Antibody (bsm-70400M) validated for, and at what dilution?
bsm-70400M is validated for WB (1:300-5000); IP (1-2ug). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD46 (Extracellular region) Antibody (bsm-70400M) react with?
Tested reactivity: Human.
How should bsm-70400M be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
What immunogen was used to raise CD46 (Extracellular region) Antibody (bsm-70400M)?
Clone (M037) was generated from a proprietary antigen related to native human CD46 expressed in NCI-H1915 lung cancer cell line.