Cdk1 (N-terminal region) Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-70418m-100ul |
| Product Name | Cdk1 (N-terminal region) Antibody |
| Applications |
WB
IP
IHC
|
| Specificity | The antibody detects a 34 kDa* band corresponding to Cdk1 on SDS-PAGE immunoblots of human SYF and HeLa cells. The antibody does not detect other Cdk family members, such as Cdk2 and Cdk5. This sequence is conserved in human and rat Cdk1, and has low homology to other Cdk family members. |
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Clone M226 was generated from a recombinant human Cdk1 protein that included amino acids residues in the N-terminal region. |
| Clonality | Monoclonal |
| Clone # | M226 |
| Isotype | IgG1 |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | P06493 |
| Synonyms | Cdc2 |
| Background | Cyclin-dependent kinases (Cdks) are a family of serine/threonine kinases that require association with regulatory subunits known as cyclins for activation. In addition, post-translational phosphorylation and dephosphorylation events regulate Cdk activity. Phosphorylation of Thr-160 in the T loop by Cdk-activating kinase (CAK) is an obligatory step in kinase activation. By contrast, phosphorylation of the Thr-14 and Tyr-15 residues by the Wee1 family of dual specificity kinases is inhibitory for the Cdks, and dephosphorylation of these residues by the Cdc25 family of phosphatases coincides with Cdk activation. Alternatively, Cdk5 appears to require different mechanisms for activation. This Cdk is activated through association with specific activators, including p35, p39, and p67. Cdk5 is primarily activated in neuronal cells, and only c-Abl kinase, rather than Wee family members, have been shown to phosphorylate Tyr-15 to regulate its activity. |
| Application Dilution | |
| WB | 1:300-5000 |
| IP | 1-2ug |
| IHC | 1:50-200 |
Frequently asked questions
Which applications is Cdk1 (N-terminal region) Antibody (bsm-70418M) validated for, and at what dilution?
bsm-70418M is validated for WB (1:300-5000); IP (1-2ug); IHC (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Cdk1 (N-terminal region) Antibody (bsm-70418M) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-70418M be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
What immunogen was used to raise Cdk1 (N-terminal region) Antibody (bsm-70418M)?
Clone M226 was generated from a recombinant human Cdk1 protein that included amino acids residues in the N-terminal region.