ERK1 (C-terminal region) Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-70488m-100ul |
| Product Name | ERK1 (C-terminal region) Antibody |
| Applications |
WB
IHC
|
| Specificity | The antibody detects a 44 kDa* protein corresponding to ERK1 on SDS-PAGE immunoblots of human A431 epithelial cells. It does not detect ERK2. This sequence is conserved in rat and mouse ERK1, and has low homology to ERK2, as well as other ERK family members. |
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Clone (M233) was generated from a recombinant human ERK1 protein that included amino acids residues in the C-terminal region. |
| Clonality | Monoclonal |
| Clone # | M233 |
| Isotype | IgG1 |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | P28482 |
| Synonyms | ERK, p42, p44, MAPK |
| Background | Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The ERK1/2 (p44/42) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines. Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK), a MAP kinase kinase (MAPKK), and a MAP kinase (MAPK). Multiple ERK1/2 MAPKKKs have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway. MEK1 and MEK2 activate ERK1 and ERK2 through phosphorylation of activation loop residues Thr-202/Tyr-204 and Thr-185/Tyr-187, respectively. ERK1/2 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases. Several downstream targets of ERK1/2 have been identified, including p90RSK and the transcription factor Elk-1. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC | 1:50-200 |
Frequently asked questions
Which applications is ERK1 (C-terminal region) Antibody (bsm-70488M) validated for, and at what dilution?
bsm-70488M is validated for WB (1:300-5000); IHC (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ERK1 (C-terminal region) Antibody (bsm-70488M) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-70488M be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
What immunogen was used to raise ERK1 (C-terminal region) Antibody (bsm-70488M)?
Clone (M233) was generated from a recombinant human ERK1 protein that included amino acids residues in the C-terminal region.