Fascin (Ser-39), Phosphospecific Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-70497m-100ul |
| Product Name | Fascin (Ser-39), Phosphospecific Antibody |
| Applications |
WB
IHC
|
| Specificity | The antibody detects a 55 kDa* band corresponding to fascin on SDS-PAGE immunoblots of mouse C2C12 cells treated with Calyculin A. This band is not observed after lambda phosphatase treatment. This sequence has high homology to the conserved site in rat and mouse fascin, as well as to the conserved region in fascin-2, but has low homology to the conserved region in fascin-3. |
| Reactivity | Human, Mouse, Rat, Chicken |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Clone M315 was generated from a phospho-Fascin (Ser-39) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding serine 39 in human fascin. |
| Modification Site | Ser-39 |
| Clonality | Monoclonal |
| Clone # | M315 |
| Isotype | IgG1 |
| Concentration | Lot Dependent |
| Purification | Antigen Affinity purification |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | Q16658 |
| Synonyms | p55 |
| Background | Fascin is an actin filament bundling protein localized to lamellipodia and filopodia where it has important roles in cell motility. Regulation of fascin occurs through PKC-mediated phosphorylation of Ser-39 in the F-actin binding site. Cell permeant peptides that block PKC phosphorylation of Ser-39 increase cell migration, while peptides that block fascin binding to F-actin alter lamellipodial morphology and cause aberrant cell motility. Studies using RNA interference of fascin show that fibroblasts have reduced number and abnormal morphology of filopodia, while Ser-39 phosphorylation status may determine filopodial frequency. In Drosophila neurons, fascin deficiency causes alterations in actin filaments and leads to abnormal morphology of developing neurons. Thus, fascin is a critical element of actin-based motility in various cell types. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC | 1:50-200 |
Frequently asked questions
Which applications is Fascin (Ser-39), Phosphospecific Antibody (bsm-70497M) validated for, and at what dilution?
bsm-70497M is validated for WB (1:300-5000); IHC (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Fascin (Ser-39), Phosphospecific Antibody (bsm-70497M) react with?
Tested reactivity: Human, Mouse, Rat, Chicken.
How should bsm-70497M be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
What immunogen was used to raise Fascin (Ser-39), Phosphospecific Antibody (bsm-70497M)?
Clone M315 was generated from a phospho-Fascin (Ser-39) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding serine 39 in human fascin.