eNOS (C-terminal region) Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-70568m-100ul |
| Product Name | eNOS (C-terminal region) Antibody |
| Applications |
WB
IHC
|
| Specificity | The antibody detects a 140 kDa* protein corresponding to eNOS on SDS-PAGE immunoblots of human umbilical vein endothelial cells. This sequence is conserved in human and rat eNOS, and has low homology to other NOS family members. |
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Clone (M221) was generated from a recombinant mouse eNOS protein that included amino acids residues in the C-terminal region. |
| Clonality | Monoclonal |
| Clone # | M221 |
| Isotype | IgG1 |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | P70313 |
| Synonyms | endothelial Nitric Oxide Synthase, eNOS, ecNOS, NOS-III, NOS3, NOSIII |
| Background | Nitric oxide (NO) has a broad range of biological activities and is implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOS), the enzymes responsible for synthesis of NO, are homodimers whose monomers are themselves two fused enzymes: a cytochrome reductase and a cytochrome that requires three cosubstrates (L-arginine, NADPH, and oxygen) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin, and heme). Several distinct NOS isoforms are produced from three distinct genes. The inducible form of NOS, iNOS (NOS-II), is Ca2+ independent and is expressed in a broad range of cell types, and two constitutive Ca2+/CaM-dependent forms of NOS: nNOS (bNOS, NOS-I) identified in neurons and eNOS (ecNOS, NOS-III) identified in endothelial cells. Regulation of eNOS activity occurs through phosphorylation at multiple sites. Phosphorylation of Ser-633 (mouse Ser-632) in the FMN binding domain increases eNOS activity and may be important for the maintenance of NO synthesis after initial activation by Ca2+ flux and Ser-1177 phosphorylation. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC | 1:50-200 |
Frequently asked questions
Which applications is eNOS (C-terminal region) Antibody (bsm-70568M) validated for, and at what dilution?
bsm-70568M is validated for WB (1:300-5000); IHC (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does eNOS (C-terminal region) Antibody (bsm-70568M) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-70568M be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
What immunogen was used to raise eNOS (C-terminal region) Antibody (bsm-70568M)?
Clone (M221) was generated from a recombinant mouse eNOS protein that included amino acids residues in the C-terminal region.