iNOS (C-terminal region) Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-70574m-100ul |
| Product Name | iNOS (C-terminal region) Antibody |
| Applications |
WB
|
| Specificity | The antibody detects a 130 kDa* protein on SDS-PAGE immunoblots of mouse macrophages (RAW264.7) treated with IFNγ and LPS, or J774A.1 cells treated with LPS only. The human iNOS sequence used has high homology with similar regions in rat and mouse iNOS. |
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Clone (M398) was generated from a recombinant protein that included amino acid residues within the C-terminal region of human iNOS. |
| Clonality | Monoclonal |
| Clone # | M398 |
| Isotype | IgG2a |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | P35228 |
| Synonyms | Nos2 nitric oxide synthase 2, inducible, macrophage, NOS, type II, NOSII, Hepatocyte |
| Background | Nitric oxide (NO) has a broad range of biological activities and is implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOS), the enzymes responsible for synthesis of NO, are homodimers whose monomers are themselves two fused enzymes: a cytochrome reductase and a cytochrome that requires three cosubstrates (L-arginine, NADPH, and oxygen) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin, and heme). Several distinct NOS isoforms are produced from three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS: nNOS (also designated bNOS, NOS-I), whose activity was first identified in neurons and eNOS (also designated ecNOS, NOS-III) first identified in endothelial cells. The inducible form of NOS, iNOS (also designated NOS-II), is Ca2+ independent and is expressed in a broad range of cell types. This form of NOS is induced after stimulation with cytokines and exposure to microbial products. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is iNOS (C-terminal region) Antibody (bsm-70574M) validated for, and at what dilution?
bsm-70574M is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does iNOS (C-terminal region) Antibody (bsm-70574M) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-70574M be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
What immunogen was used to raise iNOS (C-terminal region) Antibody (bsm-70574M)?
Clone (M398) was generated from a recombinant protein that included amino acid residues within the C-terminal region of human iNOS.