p21/CIP1/WAF1 Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-70581M-100uL |
| Product Name | p21/CIP1/WAF1 Antibody |
| Applications |
WB
IP
IHC
|
| Specificity | The antibody detects a 21 kDa* protein corresponding to the apparent molecular mass of p21 on SDS-PAGE immunoblots of human endothelial cells (HUVEC), HeLa cells, and Fibroblasts. The antibody is also useful for immunoprecipitation and immunofluorescent labeling in human cells. |
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Clone M513 was generated from a full length recombinant human p21 protein. The antibody detects human p21. |
| Clonality | Monoclonal |
| Clone # | M513 |
| Isotype | IgG2a |
| Concentration | Lot Dependent |
| Purification | Antigen Affinity purification |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | P38936 |
| Synonyms | Cyclin-dependent kinase inhibitor 1 CDK-interacting protein 1 Melanoma differentiation-associated protein 6 MDA-6 p21 CDKN1A CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1 |
| Background | The tumor suppressor protein p21/CIP1/WAF1 acts as an inhibitor of cell cycle progression. It functions in stoichiometric relationships forming heterotrimeric complexes with cyclins and cyclin-dependent kinases. In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1/S. However, p21 may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D. The carboxy-terminal region of p21 is sufficient to bind and inhibit PCNA, a subunit of DNA polymerase, and may coordinate DNA replication with cell cycle progression. Upon UV damage or during cell cycle stages when cdc2/cyclin B or CDK2/cyclin A are active, p53 is phosphorylated and upregulates p21 transcription via a p53-responsive element. Protein levels of p21 are downregulated through ubiquitination and proteasomal degradation. |
| Application Dilution | |
| WB | 1:300-5000 |
| IP | 1-2ug |
| IHC | 1:50-200 |
Frequently asked questions
Which applications is p21/CIP1/WAF1 Antibody (bsm-70581M) validated for, and at what dilution?
bsm-70581M is validated for WB (1:300-5000); IP (1-2ug); IHC (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does p21/CIP1/WAF1 Antibody (bsm-70581M) react with?
Tested reactivity: Human.
How should bsm-70581M be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
What immunogen was used to raise p21/CIP1/WAF1 Antibody (bsm-70581M)?
Clone M513 was generated from a full length recombinant human p21 protein. The antibody detects human p21.