PKCθ (N-terminal region) Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-70615m-100ul |
| Product Name | PKCθ (N-terminal region) Antibody |
| Applications |
WB
|
| Specificity | This antibody detects a 79 kDa* protein corresponding to the molecular mass of PKCθ on SDS-PAGE immunoblots of human Jurkat cell lysates. This sequence is conserved in human and rat PKCθ, and has low homology to other PKC family members. |
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Clone (M217) was generated from a recombinant mouse PKCθ protein that included amino acids residues in the N-terminal region. |
| Clonality | Monoclonal |
| Clone # | M217 |
| Isotype | IgG2a |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | A6H667 |
| Synonyms | PKC theta |
| Background | The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half interacts with PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester, while the C-terminal half contains the catalytic domain. The conventional PKC subfamily (α, β1, βII, and γ) is regulated by both Ca2+ and diacylglycerol. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters, and growth factors. The phosphorylation of multiple sites in PKCs regulates their activity |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is PKCθ (N-terminal region) Antibody (bsm-70615M) validated for, and at what dilution?
bsm-70615M is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PKCθ (N-terminal region) Antibody (bsm-70615M) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-70615M be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
What immunogen was used to raise PKCθ (N-terminal region) Antibody (bsm-70615M)?
Clone (M217) was generated from a recombinant mouse PKCθ protein that included amino acids residues in the N-terminal region.