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Lane 1: SP2\/0 Cell lysates; Lane 2: Mouse lung lysates; Lane 3: HL-60 Cell lysates; Lane 4: Mouse muscle lysates; probed with GPR84 Polyclonal Antibody, unconjugated (bs-13507R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Mouse Blood cell lysates; Lane 2: Mouse Lung lysates; Lane 3: Rat Bone lysates; Lane 4: Rat Lung lysates ; Lane 5: Rat Cerebrum lysates;Lane 6: Rat Cerebellum lysates; Lane 7: Human U937 cell lysates; Lane 8: Human U87MG cell lysates; Lane 9: Human THP-1 lysates probed with GPR84 Polyclonal Antibody, Unconjugated (bs-13507R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-13507R

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse brain lysates probed with Anti SRXN1 Polyclonal Antibody, Unconjugated (bs-8329R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C.
Lane 1: Mouse Cerebrum lysates; Lane 2: Rat Cerebrum lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Human A549 cell lysates probed with SRXN1 Polyclonal Antibody, Unconjugated (bs-8329R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-8329R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-LGALS3BP Polyclonal Antibody, Unconjugated (bs-5729R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Heart tissue lysates; Lane 2: Mouse Kidney tissue lysates; Lane 3: Rat Heart tissue lysates; Lane 4: Rat Kidney tissue lysates probed with LGALS3BP Polyclonal Antibody, Unconjugated (bs-5729R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Heart Lysates; Lane 2: Mouse Kidney Lysates; Lane 3: Rat Heart Lysates; Lane 4: Rat Kidney Lysates. Probed with LGALS3BP polyclonal Antibody, unconjugated (bs-5729R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-5729R

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

RSC96 cells probed with\\tCD9\/MRP-1 Polyclonal Antibody, Unconjugated (bs-2489R ) at 0.2ug for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Mouse splenocytes probed with\\tCD9\/MRP-1 Polyclonal Antibody, Unconjugated (bs-2489R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded mouse intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD9 Polyclonal Antibody, Unconjugated (bs-2489R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD9 Polyclonal Antibody, Unconjugated (bs-2489R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.

Catalog #1: bs-2489R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-11054R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat colitis tissue labeled with Rabbit Anti-Podoplanin\/gp36 Polyclonal Antibody (bs-1048R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat kidney tissue labeled with Rabbit Anti-Podoplanin\/gp36 Polyclonal Antibody (bs-1048R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Muscle; Probed Podoplanin Polyclonal Antibody, unconjugated (bs-1048R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bs-1048R

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin-embedded rat liver labeled with Anti-NGFR\/p75NTR Polyclonal Antibody, Unconjugated(bs-0022R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37\u00b0C
Formalin-fixed and paraffin-embedded rat liver labeled with Anti-NGFR\/p75NTR Polyclonal Antibody, Unconjugated(bs-0022R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37\u00b0C.
Formalin-fixed and paraffin embedded rat brain labeled with Anti-NGFR\/p75NTR Polyclonal Antibody, Unconjugated (bs-0161R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse brain lysates probed with Anti-p75 NGF Receptor Polyclonal Antibody (bs-0161R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.

Catalog #1: bs-0161R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0522R

Applications: WB, ELISA, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Mouse liver lysates probed with Anti-IRF3 (Ser396) Polyclonal Antibody, Unconjugated (bs-3195R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
U251 lysates probed with IRF3 (Ser396) Polyclonal Antibody, unconjugated (bs-3195R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Human heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IRF3 (Ser396) Polyclonal Antibody, Unconjugated (bs-3195R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IRF3 (Ser396) Polyclonal Antibody, Unconjugated (bs-3195R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3195R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

L1 rat spleen lysates L2 mouse heart lysates probed with Anti IL-2R gamma\/CD132 Polyclonal Antibody, Unconjugated (bs-2545R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 41kD. Observed band size:41kD.\\n
Human U937 cells probed with IL-2R gamma Polyclonal Antibody, Unconjugated (bs-2545R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
U-937 (Positive) and MCF7 (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IL-2R gamma Antibody(bs-2545R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).\r\n
SHSY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (IL-2R gamma) polyclonal Antibody, Unconjugated (bs-2545R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-2545R

Applications: WB, ELISA, FCM

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human oral squamous cell carcinoma labeled with Anti-IL-17 Polyclonal Antibody, Unconjugated(bs-2140R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: MOLT-4 cell lysates; Lane 2: U937 cell lysates probed with IL17 Polyclonal Antibody, Unconjugated (bs-2140R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Raji cell lysates probed with IL17 Polyclonal Antibody, Unconjugated (bs-2140R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: MCF-7 cell lysates probed with IL-17 Polyclonal Antibody, Unconjugated (bs-2140R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2140R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-NIS Polyclonal Antibody, Unconjugated (bs-0448R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat thyroid gland labeled with Anti-NIS Polyclonal Antibody, Unconjugated (bs-0448R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
L1 rat brain lysates L2 rat kidney lysates probed with Anti NIS Polyclonal Antibody, Unconjugated (bs-0448R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 65kD. Observed band size:65kD.
Formalin-fixed and paraffin-embedded human thyroid labeled with Rabbit Anti-NIS Polyclonal Antibody, Unconjugated(bs-448R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.

Catalog #1: bs-0448R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti- Bax Polyclonal Antibody, Unconjugated (bs-0127M) at 1:200, followed by conjugation to the secondary antibody and DAB staining.
Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Bax) polyclonal Antibody, Unconjugated (bs-0127M) 1:100, 90 minutes at 37\u00b0C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue\/cell: Sh-sy5y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Bax) polyclonal Antibody, Unconjugated (bs-0127M) 1:100, 90 minutes at 37\u00b0C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
25 ug total protein per lane of various lysates (see on figure) probed with Bax monoclonal antibody, unconjugated (bs-0127M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bs-0127m

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human rectal carcinoma labeled with Anti-Integrin beta 3\/CD61 Polyclonal Antibody, Unconjugated (bs-0342R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Human HL-60 cells probed with Integrin beta 3 Polyclonal Antibody, Unconjugated (bs-0342R) (green) at 1:100 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
HL-60 (Positive) and A431 (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Integrin beta 3 Antibody (bs-0342R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse Bone Lysates; Lane 2: Mouse Kidney Lysates. Probed with Integrin beta 3polyclonal Antibody, unconjugated (bs-0342R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0342R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-6970R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-BRLF1 Polyclonal Antibody, Unconjugated (bs-4542R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-BRLF1 Polyclonal Antibody, Unconjugated (bs-4542R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Catalog #1: bs-4542R

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human, Virus

Conjugate: Unconjugated

Catalog #1: bs-2086R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Bacteria

Conjugate: Unconjugated

Catalog #1: bs-13535R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-FZD3\/Frizzled 3 Polyclonal Antibody, Unconjugated (bs-13239R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Lane 1: mouse pancreas lysate probed with Rabbit Anti-FZD3\/Frizzled 3 Polyclonal Antibody, Unconjugated (bs-13239R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.
Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-FZD3\/Frizzled 3 Polyclonal Antibody, Unconjugated (bs-13239R) at 1:200 overnight at 4\u00b0C, followed by the secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C. DAPI(5ug\/ml) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with \\tRabbit Anti-FZD3\/Frizzled 3 Polyclonal Antibody, Unconjugated ( \\tbs-13239R ) at 1:200 overnight at 4\u00b0C, followed by the secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy)and used at 1:200 dilution for 40 minutes at 37\u00b0CDAPI(5ug\/ml) was used to stain the cell nuclei

Catalog #1: bs-13239r

Applications: WB, ELISA

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human prostate labeled with Anti-Cyclooxygenase-2 Polyclonal Antibody, Unconjugated (bs-10411R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse brain labeled with Anti-Cyclooxygenase-2 Polyclonal Antibody, Unconjugated (bs-10411R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
MCF-7 cells were stained with Cyclooxygenase Polyclonal Antibody, Unconjugated(bs-10411R) at 1:500 in PBS and incubated for two hours at 37\u00b0C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.
Lane 1: Recombinant human COX2 protein probed with Cyclooxygenase 2 Polyclonal Antibody, Unconjugated (bs-10411R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-10411R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: Huh7 probed with Rabbit Anti-CD44 Polyclonal Antibody, Unconjugated (bs-0521R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti-CD44 Polyclonal Antibody (bs-0521R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Human Raji cells probed with CD44 Polyclonal Antibody, Unconjugated (bs-0521R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
Lane 1: Human Hela cell lysates; Lane 2: Human PANC-1 cell lysates; Lane 3: Human HUVEC cell lysates; Lane 4: Human A549 cell lysates probed with CD44 Polyclonal Antibody, Unconjugated (bs-0521R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0521R

Applications: WB, ELISA, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Rabbit

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: rat sciatic nerve labeled with Anti-AGER\/RAGE Polyclonal Antibody, Unconjugated (bs-0177R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
NIH\/3T3 cells probed with Rabbit Anti-RAGE Polyclonal Antibody (bs-0177R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated Secondary Antibody.\\n
Raji Cells lysates probed with RAGE Polyclonal Antibody, unconjugated (bs-0177R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.
MCF7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with RAGE Polyclonal Antibody(bs-0177R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0177R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat tongue labeled with Anti- Insulin Receptor alpha Polyclonal Antibody, Unconjugated(bs-0047R) 1:200 followed by conjugation to the secondary antibody and DAB staining
HL-60 cells probed with Insulin Receptor alpha Antibody, unconjugated (bs-0047R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)
Paraformaldehyde-fixed, paraffin embedded Rat small intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Insulin Receptor alpha Polyclonal Antibody, Unconjugated (bs-0047R) at 1:200 overnight at 4\u00b0C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Insulin Receptor alpha Polyclonal Antibody, Unconjugated (bs-0047R) at 1:200 overnight at 4\u00b0C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.

Catalog #1: bs-0047R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-0118r

Applications: IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin-embedded human skin psoriasis labeled with Rabbit Anti- PPARr gamma Polyclonal Antibody, Unconjugated (bs-0530R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse stomach lysates probed with PPAR gamma Polyclonal Antibody, unconjugated (bs-0530R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.
Sheep heart lysates probed with PPAR gamma Polyclonal Antibody, unconjugated (bs-0530R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
293T Cells probed with PPAR gamma Polyclonal Antibody, unconjugated (bs-0530R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:20000 for 60 minutes at 37\u00b0C.

Catalog #1: bs-0530r

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-Endomucin Polyclonal Antibody, Unconjugated (bs-5884R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Lane 1: Mouse heart lysates; Lane 2: Mouse lung lysates probed with Endomucin Polyclonal Antibody, Unconjugated (bs-5884R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Endomucin) polyclonal Antibody, Unconjugated (bs-5884R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-5884R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Transfected HeLa cell lysates probed with Anti-CSFV Envelope glycoprotein E2 Polyclonal Antibody, Unconjugated (bs-4527R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.
Lane 1: Recombinant CSFV E2 protein (200ng) (bs-20116P). Probed with CSFV E2 polyclonal Antibody, unconjugated (bs-4527R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-4527R

Applications: WB, ELISA

Reactivity: CSFV

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human kidney labeled with Anti-Phospho-SGK1 (Ser422) Polyclonal Antibody, Unconjugated (bs-3396R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse pancreas labeled with Anti-Phospho-SGK1 (Ser422) Polyclonal Antibody, Unconjugated (bs-3396R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse Muscle lysates probed with SGK1 (Ser422) Polyclonal Antibody, Unconjugated (bs-3396R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Pancreas lysates probed with SGK1 (Ser422) Polyclonal Antibody, Unconjugated (bs-3396R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3396R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2650R

Applications: WB, ELISA

Reactivity: Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with WNT2B Polyclonal Antibody, Unconjugated (bs-1946R) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Heart tissue lysates; Lane 3: Rat Cerebrum tissue lysates; Lane 4: Rat Heart tissue lysates probed with WNT2B Polyclonal Antibody, Unconjugated (bs-1946R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1946R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat colon labeled with Anti-FOXJ1\/HFH-4 Polyclonal Antibody, Unconjugated (bs-1775R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Fork-head box protein J1, FOXJ1 Polyclonal Antibody, Unconjugated (bs-1775R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Lane 1: Mouse Testis lysates; Lane 2: Rat Testis lysates probed with HFH4 Polyclonal Antibody, Unconjugated (bs-1775R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (FOXJ1) Polyclonal Antibody, Unconjugated (bs-1775R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-1775R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1:mouse kidney lysates Lane 2: mouse brain lysates probed with Anti-MC-1R\/MC1 Receptor Polyclonal Antibody, Unconjugated (bs-1419R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 35kD. Observed band size: 35kD.
paraformaldehyde-fixed, paraffin embedded human melanoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-MC-1R Polyclonal Antibody, Unconjugated (bs-1419R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Human 293T Cells were fixed with 2% PFA (10min at room temperature) and then permeabilized with 90% ice-cold methanol for 30min on ice. The cells were then incubated in 2% BSA + 10% goat serum to block non-specific protein-protein interactions for 15 min. Cells were stained with MC-1R\/MC1 Polyclonal Antibody, Alexa Fluor 488 Conjugated (bs-1419R-A488) at 1:100 for 30 min on ice. Primary Antibody staining (green) is compared to unstained cells (dark blue), and isotype control (orange).
Lane 1: MOLT-4 Cells; Probed with MC-1R\/MC1 Polyclonal Antibody, Unconjugated (bs-1419R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bs-1419R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human gastric adenocarcinoma labeled with Rabbit Anti-GPR30 Polyclonal Antibody (bs-1380R) at 1:300 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human rectal carcinoma tissue labeled with Rabbit Anti-GPR30 Polyclonal Antibody (bs-1380R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human rectal carcinoma tissue labeled with Rabbit Anti-GPR30 Polyclonal Antibody (bs-1380R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human rectal carcinoma tissue labeled with Rabbit Anti-GPR30 Polyclonal Antibody (bs-1380R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-1380R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded mouse small intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SLC38A2 Polyclonal Antibody, Unconjugated (bs-12125R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Mouse intestine lysates probed with SLC38A2 Polyclonal Antibody, Unconjugated (bs-12125R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Liver tissue lysates; Lane 2: Rat Lung tissue lysates probed with SLC38A2 Polyclonal Antibody, Unconjugated (bs-12125R) at 1:200 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-12125R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat articular cartilage labeled with Anti-MMP-13 Polyclonal Antibody (bs-0575R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Generously provided by Markus Linder from Medical University Vienna as part of the Bioss Discovery Program. Formalin-fixed, paraffin embedded, and decalcified in EDTA mouse bone labeled with Anti-MMP-13 Polyclonal Antibody, Unconjugated (bs-0575R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human bladder cancer labeled with Rabbit Anti-MMP-13 Polyclonal Antibody, Unconjugated (bs-0575R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Human PC3 lysates probed with Rabbit Anti-MMP13 Polyclonal Antibody, Unconjugated (bs-0575R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-0575R

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat liver tissue labeled Anti-Insulin Receptor\/CD220 Polyclonal Antibody, Unconjugated (bs-0681R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Raji cells probed with Insulin Receptor Polyclonal Antibody, unconjugated (bs-0681R) at 1:100 dilution for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange)
Lane 1: Human HepG2 cell lysates; Lane 2: Human 293T cell lysates; Lane 3: Human LOVO cell lysates; Lane 4: Human MOLT-4 cell lysates; Lane 5: Human SW480 cell lysates; Lane 6: Human K562 cell lysates probed with Insulin Receptor Polyclonal Antibody, Unconjugated (bs-0681R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0681R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-41414m

Applications: WB, ELISA

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Lane 1: SARS-CoV-2 N protein(Cat# bs-41408P) at 50ng; Lane 2: SARS-CoV-2 N protein(Cat# bs-41408P) at 25ng; Lane 3: SARS-CoV-2 N protein(Cat# bs-41408P) at 5ng; Lane 4: SARS-CoV-2 N protein(Cat# bs-41408P) at 2ng ; Lane 5: SARS-CoV-2 N protein(Cat# bs-41408P) at 1ng;Lane 6: SARS-CoV-2 N protein(Cat# bs-41408P) at 0.5ng probed with SARS-CoV-2 N protein monoclonal Antibody, Unconjugated (bsm-41413M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Recombinant SARS-CoV-2 N protein (WT) (His Tag) (bs-41408P); Lane 2: Recombinant SARS-CoV-2 N protein (Q9H, P67S, P80R, P151L, S183Y) (His Tag) (bs-41451P); Lane 3: Recombinant SARS-CoV-2 N protein (D3L, P13T, D103Y, D128Y, H145Y, R203K, G204R, T205I, S235F) (His Tag) (bs-41452P); Lane 4: Recombinant SARS-CoV-2 N protein (Del204, Del215) (His Tag) (bs-41491P); Lane 5: Recombinant SARS-Cov-2 N protein (R203M, D377Y) (His Tag) (bs-41492P); Lane 6: Recombinant SARS-Cov-2 (Omicron, B.1.1.529) N protein (P13L, E31del, R32del, S33del, R203K, G204R) (N-His Tag) (bs-41494P) probed with SARS-CoV-2(2019-nCoV)Nucleocapsid Monoclonal Antibody, Unconjugated (bsm-41413M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-41413m

Applications: WB, ELISA, FCM

Reactivity:

Conjugate: Unconjugated

Lane 1: SARS-CoV-2 N protein(Cat# bs-41408P) at 50ng; Lane 2: SARS-CoV-2 N protein(Cat# bs-41408P) at 25ng; Lane 3: SARS-CoV-2 N protein(Cat# bs-41408P) at 5ng; Lane 4: SARS-CoV-2 N protein(Cat# bs-41408P) at 2ng ; Lane 5: SARS-CoV-2 N protein(Cat# bs-41408P) at 1ng;Lane 6: SARS-CoV-2 N protein(Cat# bs-41408P) at 0.5ng probed with SARS-CoV-2 N protein monoclonal Antibody, Unconjugated (bsm-41412M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Recombinant SARS-CoV-2 N protein (WT) (His Tag) (bs-41408P); Lane 2: Recombinant SARS-CoV-2 N protein (Q9H, P67S, P80R, P151L, S183Y) (His Tag) (bs-41451P); Lane 3: Recombinant SARS-CoV-2 N protein (D3L, P13T, D103Y, D128Y, H145Y, R203K, G204R, T205I, S235F) (His Tag) (bs-41452P); Lane 4: Recombinant SARS-CoV-2 N protein (Del204, Del215) (His Tag) (bs-41491P); Lane 5: Recombinant SARS-Cov-2 N protein (R203M, D377Y) (His Tag) (bs-41492P); Lane 6: Recombinant SARS-Cov-2 (Omicron, B.1.1.529) N protein (P13L, E31del, R32del, S33del, R203K, G204R) (N-His Tag) (bs-41494P) probed with SARS-CoV-2(2019-nCoV)Nucleocapsid Monoclonal Antibody, Unconjugated (bsm-41412M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-41412m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Nrf2(S40) (7G4) Monoclonal Antibody(bsm-52179R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (phospho-Nrf2 (Ser40)) monoclonal Antibody, Unconjugated (bsm-52179R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-Nrf2 (Ser40) Monoclonal Antibody, Unconjugated(bsm-52179R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-Nrf2 (Ser40) Monoclonal Antibody, Unconjugated(bsm-52179R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.

Catalog #1: bsm-52179r-TR

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-7175R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-5013R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-4940R

Applications: WB, ELISA, FCM, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-4595R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Mouse lung lysates probed with CD74 Polyclonal Antibody Unconjugated (bs-2518R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Raji lysates probed with CD74 Polyclonal Antibody, Unconjugated (bs-2518R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-2518R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney labeled with Anti-EPO Polyclonal Antibody, Unconjugated (bs-2343R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with EPO Polyclonal Antibody, Unconjugated (bs-2343R ) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (EPO) Polyclonal Antibody, Unconjugated (bs-2343R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (EPO) Polyclonal Antibody, Unconjugated (bs-2343R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-2343R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), dot-blot

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human endometrium labeled with Anti-UCP-2 Polyclonal Antibody, Unconjugated (bs-1926R) at 1:400 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with UCP-2 Polyclonal Antibody, Unconjugated (bs-1926R) at 1:400 overnight at 4\u00b0C, DAB staining.
Human Hela Cells were fixed with 2% PFA (10min at room temperature) and then permeabilized with 90% ice-cold methanol for 30min on ice. The cells were then incubated in 2% BSA + 10% goat serum to block non-specific protein-protein interactions for 15 min at room temperature. Cells were stained with UCP-2 Polyclonal Antibody, Unconjugated (bs-1926R) at 1:100 for 30 min at room temperature. The secondary antibody, F(ab\u2019)2 fragment Goat anti-rabbit IgG-FITC, was used for 40 min at room temperature. Primary Antibody staining (green) is compared to unstained cells (dark blue), secondary only (light blue) and isotype control (orange).
Lane 1: Raw264.7 cell lysates probed with UCP-2 Polyclonal Antibody, Unconjugated (bs-1926R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1926R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat colon tissue labeled with Anti CD177\/NB1 Polyclonal Antibody, Unconjugated (bs-1482R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: Hela Cells; Lane 2: K562 Cells; Lane 3: MCF-7 Cells; Lane 4: 293T Cells; Lane 5: Sp2\/0 Cells; Lane 6: NIH\/3T3 Cells; 40ug of lysate loaded into each lane; Probed with CD177 Polyclonal Antibody, unconjugated (bs-1482R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Mouse Spleen lysates; Lane 2: Mouse Raw264.7 cell lysates; Lane 3: Rat Lymph node lysates probed with CD177 Polyclonal Antibody, Unconjugated (bs-1482R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1482R

Applications: WB, IHC-P

Reactivity: Mouse, Rat

Conjugate: Unconjugated