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Lane 1: Human MCF-7 cell lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human A549 cell lysates probed with RAR gamma Polyclonal Antibody, Unconjugated (bs-6218R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (RAR gamma) polyclonal Antibody, Unconjugated (bs-6218R) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-6218r

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig

Conjugate: Unconjugated

Catalog #1: bs-6218r-bf555

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig

Conjugate: AbBy Fluor-555

Catalog #1: bs-6218r-bf405

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig

Conjugate: AbBy Fluor-405

Catalog #1: bs-6218r-apc

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig

Conjugate: APC

Catalog #1: bs-6218r-apc-cy55

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig

Conjugate: APC-Cy5.5

Catalog #1: bs-5485r-rbitc

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: RBITC

Catalog #1: bs-5485r-pe-cy55

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: PE-Cy5.5

Catalog #1: bs-5485r-percp-cy55

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: PerCP-Cy5.5

Catalog #1: bs-5485r-percp

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: PerCP

Catalog #1: bs-5485r-percp-cy7

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: PerCP-Cy7

Catalog #1: bs-5485r-hrp

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: HRP

Catalog #1: bs-5485r-pe-cy5

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: PE-Cy5

Catalog #1: bs-5485r-fitc

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: FITC

Catalog #1: bs-5485r-pe-cy7

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: PE-Cy7

Catalog #1: bs-5485r-pe

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: PE

Catalog #1: bs-5485r-cy7

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Cy7

Catalog #1: bs-5485r-cy3

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Cy3

Catalog #1: bs-5485r-cy5

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Cy5

Catalog #1: bs-5485r-cy55

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Cy5.5

Catalog #1: bs-5485r-bf750

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: AbBy Fluor-750

Catalog #1: bs-5485r-biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Biotin

Catalog #1: bs-5485r-bf647

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: AbBy Fluor-647

Catalog #1: bs-5485r-apc-cy7

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: APC-Cy7

Catalog #1: bs-5485r-bf680

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: AbBy Fluor-680

Catalog #1: bs-5485r-bf594

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: AbBy Fluor-594

Catalog #1: bs-5485r-bf555

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: AbBy Fluor-555

Catalog #1: bs-5485r-bf488

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: AbBy Fluor-488

Catalog #1: bs-5485r-bf405

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: AbBy Fluor-405

Catalog #1: bs-5485r-bf350

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: AbBy Fluor-350

Catalog #1: bs-5485r-apc-cy55

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: APC-Cy5.5

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti Phospho-MEF2A(Thr319) Polyclonal Antibody, Unconjugated (bs-5485R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 mouse embryo lysates L2 mouse brain lysates probed with Anti Phospho-MEF2A(Thr319) Polyclonal Antibody, Unconjugated (bs-5485R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 54kD. Observed band size:54kD.\\n
Antigen: bs-5485P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
25 ug total protein per lane of various lysates (see on figure) probed with Phospho-MEF2A (Thr319) polyclonal antibody, unconjugated (bs-5485R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bs-5485r

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Unconjugated

Catalog #1: bs-5485r-apc

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: APC

Catalog #1: bs-4635r-pe-cy7

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: PE-Cy7

Catalog #1: bs-4635r-percp-cy7

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: PerCP-Cy7

Catalog #1: bs-4635r-percp-cy55

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: PerCP-Cy5.5

Catalog #1: bs-4635r-rbitc

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: RBITC

Catalog #1: bs-4635r-percp

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: PerCP

Catalog #1: bs-4635r-pe-cy55

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: PE-Cy5.5

Catalog #1: bs-4635r-bf750

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: AbBy Fluor-750

Catalog #1: bs-4635r-pe-cy5

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: PE-Cy5

Catalog #1: bs-4635r-pe

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: PE

Catalog #1: bs-4635r-fitc

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: FITC

Catalog #1: bs-4635r-cy5

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Cy5

Catalog #1: bs-4635r-cy3

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Cy3

Catalog #1: bs-4635r-hrp

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: HRP

Catalog #1: bs-4635r-cy7

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Cy7

Catalog #1: bs-4635r-cy55

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Cy5.5

Catalog #1: bs-4635r-bf647

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: AbBy Fluor-647