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Formalin-fixed and paraffin embedded rat kidney labeled with Anti Catalase Polyclonal Antibody, Unconjugated (bs-6874R) followed by conjugation to the secondary antibody and DAB staining
L1 rat kidney lysates L2 rat brain lysates probed with Anti Catalase Polyclonal Antibody, Unconjugated (bs-6874R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 58kD. Observed band size: 58kD
Mouse liver lysates probed with Rabbit Anti-Catalase Polyclonal Antibody, Unconjugated (bs-6874R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
HL-60 cells were fixed with 4% PFA for 10min at room temperature,20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Catalase Polyclonal Antibody (bs-6874R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-6874R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

RSC96 cells probed with Rabbit Anti-ADORA2B Polyclonal Antibody, PE Conjugated (bs-5900R-PE) at 1:30 for 40 minutes (green) compared to control cells (blue).
Mouse brain lysates probed with Rabbit Anti-ADORA2B Polyclonal Antibody (bs-5900R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ADORA2B Polyclonal Antibody, Unconjugated (bs-5900R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ADORA2B Polyclonal Antibody, Unconjugated (bs-5900R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-5900R

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: rat brain lysates probed with Rabbit Anti-RhoA(Ser188) Polyclonal Antibody, Unconjugated (bs-5330R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Molt-4 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with RhoA(Ser188) Polyclonal Antibody(bs-5330R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RhoA(Ser188) Polyclonal Antibody, Unconjugated (bs-5330R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-5330R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti CD8 Polyclonal Antibody, Unconjugated (bs-4790R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CD8 Polyclonal Antibody, Unconjugated (bs-4790R) at 1:200 followed by secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (bs-0295G-Cy3) used at 1:200
U251 cells probed with CD8 Polyclonal Antibody, Unconjugated (bs-4790R) for 30 minutes followed by incubation with a conjugated secondary (FITC Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Catalog #1: bs-4790R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-4507r

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Phospho-Stat2 (Tyr690) Polyclonal Antibody, Unconjugated (bs-3428R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse brain lysates probed with Stat2 (Tyr690) Polyclonal Antibody, unconjugated (bs-3428R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bs-3428R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Hela (Positive) and Molt-4 (Negative control) cells (black) were fixed with 4% PFA for 10min at room temperature, permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with FoxO1 (Ser256) Antibody (bs-3142R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Phospho-FoxO1 (Ser256) Polyclonal Antibody(bs-3142R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FoxO1 (Ser256) Polyclonal Antibody, Unconjugated (bs-3142R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FoxO1 (Ser256) Polyclonal Antibody, Unconjugated (bs-3142R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3142R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Unconjugated

Lane 1: Mouse Brain lysates; Lane 2: Mouse Cerebellum lysates; Lane 3: Mouse Kidney lysates; Lane 4: Mouse Heart cell lysates ; Lane 5: Mouse Muscle lysates probed with CRHR2 Polyclonal Antibody, Unconjugated (bs-2792R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human Hela cell lysates; Lane 2: Human 293T cell lysates; Lane 3: Human HL60 cell lysates probed with CRHR2 Polyclonal Antibody, Unconjugated (bs-2792R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2792R

Applications: WB, ELISA, IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon cancer labeled with Anti-CD151\/MER2\/PETA-3 Polyclonal Antibody, Unconjugated (bs-2524R) at 1:200 followed by conjugation to the secondary antibody
Mouse heart lysates probed with Rabbit Anti-CD151 Polyclonal Antibody, Unconjugated (bs-2524R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C

Catalog #1: bs-2524R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Images provided by the Independent Validation Program (badge 29798).\\n\\nLane one: HeLa cell lysates irradiated with 25 Joules\/m2\\nUV-C Lane 2: Non-irradiated HeLa cell lysates probed with Rabbit Anti-p38 MAPK (Thr180 + Tyr182) Polyclonal Antibody, Unconjugated (bs-2210R) at 1:100 overnight at 4˚C. Followed by conjugation to secondary antibody at 1:10000 for 60 min at 37˚C.
Human MCF7 lysates probed with Rabbit Anti-p38 MAPK (Thr180+Tyr182) Polyclonal Antibody, Unconjugated (bs-2210R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (phospho-p38 MAPK (Thr180 + Tyr182)) polyclonal Antibody, Unconjugated (bs-2210R) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with p38 MAPK (Thr180 + Tyr182) Polyclonal Antibody, Unconjugated (bs-2210R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-2210R

Applications: WB, ELISA, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat, Chicken, Goat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse spleen tissue labeled with Anti CCR6\/CD196 Polyclonal Antibody, Unconjugated (bs-1542R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CCR6 Polyclonal Antibody, Unconjugated (bs-1542R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary (bs-0295G-AF594) at [1:500] for 90 minutes and DAPI staining of the nuclei.
HepG2 cells were\u00a0 incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with CCR6 Polyclonal Antibody(bs-1542R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).
Lane 1: Human HepG2 cell lysates; Lane 2: Human MCF-7 cell lysates probed with CCR6 Polyclonal Antibody, Unconjugated (bs-1542R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1542R

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded rat ovary tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-P2Y9 Polyclonal Antibody, Unconjugated (bs-12074R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat ovary tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-P2Y9 Polyclonal Antibody, Unconjugated (bs-12074R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Human HL60 cell lysates probed with Rabbit Anti-P2Y9 Polyclonal Antibody, Unconjugated (bs-12074R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:10000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded mouse embryo tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-P2Y9 Polyclonal Antibody, Unconjugated (bs-12074R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining

Catalog #1: bs-12074R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

A431 (Positive) and Huvec (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CHRM4\/mAChR M4 Antibody (bs-11998R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
A431 cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with \r\nCHRM4\/mAChR M4 Polyclonal Antibody(bs-11998R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Human U-2 OS cell lysates; Lane 2: Human THP-1 cell lysates; Lane 3: Human MCF-7 cell lysates; Lane 4: Human A549 cell lysates probed with CHRM4 Polyclonal Antibody, Unconjugated (bs-11998R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-11998R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human cervical carcinoma labeled with Anti-IGFBP2 Polyclonal Antibody, Unconjugated (bs-1108R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human glioma labeled with Anti-IGFBP2 Polyclonal Antibody, Unconjugated (bs-1108R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: Rat Brain lysates; Lane 2: Human Colon Carcinoma lysates; Probed with IGFBP2 Polyclonal Antibody, Unconjugated (bs-1108R) at 1:200 overnight at 4˚C. Followed by secondary antibody (bs-0295G-HRP) incubation at 1:3000 for 90 min at 37˚C.
Lane 1: Human MOLT4 cell lysates; Lane 2: Human MCF-7 cell lysates probed with IGFBP2 Polyclonal Antibody, Unconjugated (bs-1108R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1108R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-1121R

Applications: IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-CD200R\/Orexin receptor Polyclonal Antibody, Unconjugated (bs-1095R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat kidney labeled with Anti-CD200R\/Orexin receptor Polyclonal Antibody, Unconjugated (bs-1095R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: Mouse cerebral cortex lysates; Lane 2: Mouse hippocampus lysates; Lane 3: Mouse brain lysates; Lane 4: rat Brain lysates probed with Orexin receptor 1+2 Polyclonal Antibody, Unconjugated (bs-1095R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Rat Cerebrum tissue lysates; Lane 4: Rat Cerebellum tissue lysates probed with Orexin receptor 1+2 Polyclonal Antibody, Unconjugated (bs-1095R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1095R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-10406R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse tooth germ tissue labeled with Anti-Nanog Polyclonal Antibody (bs-0829R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Lane 1: A549 cell lysates probed with Rabbit Anti-Nanog Polyclonal Antibody, Unconjugated (bs-0829R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
HeLa cells probed with Estrogen Receptor alpha + beta Antibody, unconjugated (bs-0829R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)

Catalog #1: bs-0829R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: human endometrium Anti-TrK-A\/NTRK1 Polyclonal Antibody (bs-0193R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Mouse Brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TrkA Polyclonal Antibody, Unconjugated (bs-0193R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse Brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (TrkA) Polyclonal Antibody, Unconjugated (bs-0193R) at 1:400 overnight at 4\u00b0C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-Cy3) for 90 minutes, and DAPI for nuclei staining.
U251 lysates probed with TrkA Polyclonal Antibody, Unconjugated (bs-0193R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-0193R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human kidney labeled with Rabbit Anti-phospho-CREB-1(Ser133) Polyclonal Antibody (bs-0036R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded mouse hippocampus labeled with Rabbit Anti-phospho-CREB-1(Ser133) Polyclonal Antibody, Unconjugated 1:500 followed by conjugation to the secondary antibody
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-phospho-CREB-1(Ser133) Polyclonal Antibody, Unconjugated 1:500 followed by conjugation to the secondary antibody
Formalin-fixed and paraffin-embedded rat brain labeled with Anti-phospho-CREB-1(Ser133) Polyclonal Antibody, Unconjugated(bs-0036R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.

Catalog #1: bs-0036R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0011R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse embryonic bone labeled with Anti-Osteocalcin Polyclonal Antibody, Unconjugated (bs-0470R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Generously provided by Markus Linder from Medical University Vienna as part of the Bioss Discovery Program. Formalin-fixed, paraffin embedded, and decalcified in EDTA mouse osteosarcoma labeled with Anti-Osteocalcin Polyclonal Antibody, Unconjugated (bs-0470R) at 1:100 followed by conjugation to the secondary antibody and DAB staining
Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Osteocalcin Polyclonal Antibody(bs-0470R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0470R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-9686R

Applications: IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Raji cell lysates probed with RIPK-1 Polyclonal Antibody, Unconjugated (bs-5805R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Cerebrum cell lysates probed with RIPK-1 Polyclonal Antibody, Unconjugated (bs-5805R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RIPK-1 Polyclonal Antibody, Unconjugated (bs-5805R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RIPK-1 Polyclonal Antibody, Unconjugated (bs-5805R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-5805R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin-embedded Rat Colitis; Antigen retrieval performed with citrate buffer (0.01M, pH 6.0), boiling for 15min; Block endogenous peroxidase with 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37\u2103 for 20 min; Antibody incubation with IL-1R1 (Tyr496) Polyclonal Antibody, Unconjugated (bs-5394R) at 1:200, overnight at 4\u2103 followed by conjugated secondary antibody, DAB and counterstaining.
Lane 1: Mouse Intestinal lysate; Probed with IL-1R1 (Tyr496) Polyclonal Antibody, Unconjugated (bs-5394R) at 1:300 overnight at 4˚C. Followed by incubation with conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
MCF-7 cells were fixed with 70% methanol (Overnight at 4\u2103). The cells were then incubated in 1X PBS \/ 2% BSA \/ 10% goat serum to block non-specific protein-protein interactions for 15 min at room temperature. Primary incubation with IL-1R1 (Tyr496) Polyclonal Antibody, Unconjugated (bs-5349R) at 1:100 (1\u03bcg \/10^6 cells) for 30 minutes at room temp. A PE-conjugated secondary antibody (bs-0295G-PE) was used for 40 min at room temperature. The graph compares the primary antibody (green) to unstained cells (dark blue), secondary only (light blue), and isotype control (bs-0295P; orange).

Catalog #1: bs-5394R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human breast cancer labeled with Rabbit Anti-AKT1\/2\/3 (Thr305\/308\/309) Polyclonal Antibody, Unconjugated (bs-5182R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-AKT1\/2\/3 (Thr305\/308\/309) Polyclonal Antibody, Unconjugated (bs-5182R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse lung lysates probed with Rabbit Anti-Akt1\/2\/3 (Thr305\/308\/309) Polyclonal Antibody, Unconjugated (bs-5182R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: HepG2 cell lysates probed with phospho-AKT1 + AKT2 + AKT3 (Thr308+Thr309+Thr305) Polyclonal Antibody, Unconjugated (bs-5182R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C. The upper band could be the fully glycosylated form of the target protein.

Catalog #1: bs-5182R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-4727R-FITC

Applications: WB, FCM

Reactivity: Human

Conjugate: FITC

Antigen: bs-4870P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Lane 1: mouse liver lysates Lane 2: mouse brain lysates probed with Anti MS4A2 Polyclonal Antibody, Unconjugated (bs-4870R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 27kD. Observed band size: 27kD.

Catalog #1: bs-4870R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat spleen labeled with Anti-HAS1 Polyclonal Antibody, Unconjugated (bs-2946R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
U-87MG (Positive) and A431 (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with HAS1 Antibody (bs-2946R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).\r\n

Catalog #1: bs-2946R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2177R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-1768R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-IDO2 Polyclonal Antibody, Unconjugated (bs-16640R) at 1:500 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: Mouse Placenta tissue lysates; Lane 2: Mouse Liver tissue lysates; Lane 3: Mouse Lung tissue lysates; Lane 4: Mouse Spleen tissue lysates ; Lane 5: Rat Liver tissue lysates ; Lane 6: Rat Spleen tissue lysates probed with INDOL1 Polyclonal Antibody, Unconjugated (bs-16640R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-16640R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: rat brain lysates Lane 2: rat kidney lysates probed with Anti MCR Polyclonal Antibody, Unconjugated (bs-1850R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 108kD. Observed band size: 108kD.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Mineralocorticoid receptor Polyclonal Antibody, Unconjugated (bs-1850R) at 1:400 overnight at 4\u00b0C, followed by secondary and DAB staining.
Molt-4 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with \r\nMineralocorticoid receptor Polyclonal Antibody(bs-1850R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Mouse spleen cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Mineralocorticoid receptor Polyclonal Antibody(bs-1850R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-1850R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-Hyaluronidase-1 Polyclonal Antibody, Unconjugated (bs-1235R) at 1:300 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human hepatoma labeled with Anti-Hyaluronidase 1 Polyclonal Antibody, Unconjugated (bs-1235R) followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-1235R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human glioma labeled with ASCL1 Polyclonal Antibody, Unconjugated (bs-1155R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with ASCL1 Polyclonal Antibody, Unconjugated (bs-1155R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
A549 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ASCL1 Antibody(bs-1155R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (MASH1) polyclonal Antibody, Unconjugated (bs-1155R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-1155R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-HGF Polyclonal Antibody, Unconjugated(bs-1025R) at 1:200, followed by conjugation to the secondary antibody and DAB staining\\n
Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HGF Polyclonal Antibody, Unconjugated (bs-1025R) at 1:400 overnight at 4\u00b0C, DAB staining.
25 ug total protein per lane of various lysates (see on figure) probed with HGF polyclonal antibody, unconjugated (bs-1025R) at 1:200 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bs-1025R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human rectal carcinoma labeled with Rabbit Anti ANGPTL1\/Angiopoietin 1\/ANG-1 Polyclonal Antibody, Unconjugated (bs-0800R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Angiopoietin-1) Polyclonal Antibody, Unconjugated (bs-0800R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Lane 1: Mouse Lung lysates; Lane 2: U87MG cell lysates; Lane 3: Mouse Heart lysates; Lane 4: Mouse Kidney lysates probed with Angiopoietin 1 Polyclonal Antibody, Unconjugated (bs-0800R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: K562 cell lysates; Lane 2: U87MG cell lysates; Lane 3: Hela cell lysates; Lane 4: Huvec cell lysates probed with Angiopoietin 1 Polyclonal Antibody, Unconjugated (bs-0800R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0800R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human kidney carcinoma labeled with Anti-Endostatin Polyclonal Antibody (bs-0547R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Liver; Probed with Endostatin Polyclonal Antibody, Unconjugated (bs-0547R) at 1:500, overnight at 4\u00b0C followed by incubation with a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bs-0547R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-Cytochrome C Polyclonal Antibody, Unconjugated (bs-0013R) 1:300 followed by conjugation to the secondary antibody and DAB staining
L1 mouse brain lysates L2 mouse lung lysates probed with Anti Cytochrome C Polyclonal Antibody, Unconjugated (bs-0013R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 26kD. Observed band size:26kD.
Image kindly submitted by an end-user via Funakoshi. 4% Paraformaldehyde fixed HeLa cells stained with Rabbit Anti-Cytochrome C Polyclonal Antibody (bs-0013R) at 1:100 for 2 hours at room temperature
HepG2 cells probed with Cytochrome C Polyclonal Antibody, unconjugated (bs-0013) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange).

Catalog #1: bs-0013R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney labeled with Anti-GAPDH Polyclonal Antibody, Unconjugated (bs-0459R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
This image was generously provided by Loyda M. Mel\u00e9ndez at the University of Puerto Rico Medical Sciences Campus.\\nA total of 30ug of monocyte-derived macrophage lysates were reduced and denatured in 1X Laemli Buffer with beta-mercaptoethanol. Samples were loaded into a TGX 4-20% gel, at 150V for 1 hour. Proteins were transferred to a PVDF membrane and probed for proteins of interest. The membrane was re-probed with Rabbit Anti-GAPDH Polyclonal Antibody (bs-0459R) in 5% milk at 1:250 dilution overnight at 4\u00b0C. The next day, the membrane was washed with TBS-T and incubated with goat anti-rabbit HRP conjugated secondary antibody for 1 hour at room temperature. The membrane was exposed for 18 seconds.\\n
MCF-7 cell lysates probed with Rabbit Anti-GAPDH Polyclonal Antibody (bs-0459R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP ) at 1:5000 for 90 min at 37˚C.
Mouse muscle lysates probed with Rabbit Anti-GAPDH Polyclonal Antibody (bs-0459R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP ) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-0459R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse small intestine tissue labeled with Anti-CD10 Polyclonal Antibody (bs-0527R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
A549 cells probed with CD10 Polyclonal Antibody, Unconjugated (bs-0527R) at 1:20 for 30 minutes followed by incubation with a conjugated secondary (bs-0295G-PE) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Human Hela cells probed with CD10 Polyclonal Antibody, Alexa Fluor 647 conjugated (bs-0527R-A647) (green) at 1:20 for 30 minutes compared to unstained cells (blue) and isotype control (orange).
Raji lysates probed with CD10 Polyclonal Antibody, Unconjugated (bs-0527R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C

Catalog #1: bs-0527r

Applications: WB

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-GLUT1 Polyclonal Antibody (bs-0472R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Image was kindly submitted by Dr. Lelisa Gemta from University of Virginia. Mouse splenocytes stained with Rabbit Anti-GLUT1 Polyclonal Antibody, PE conjugated (bs-0472R-PE)at 1:50.
Lane 1: Mouse Heart lysates; Lane 2: Mouse Cerebrum lysates; Lane 3: Mouse Muscle lysates; Lane 4: Rat Cerebrum lysates probed with GLUT1 Polyclonal Antibody, Unconjugated (bs-0472R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0472R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (MHC class I) Monoclonal Antibody, Unconjugated (bsm-52827R) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (MHC class I) Monoclonal Antibody, Unconjugated (bsm-52827R) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Human HeLa cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human U266 cell lysates probed with MHC class I Monoclonal Antibody, Unconjugated (bsm-52827R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-52827r

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-9413R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-8238R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-6895R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti EphA7\/Eph receptor A7 Polyclonal Antibody, Unconjugated (bs-7034R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Rat brain lysates probed with Rabbit Anti-Eph receptor A7 Polyclonal Antibody, Unconjugated (bs-7034R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 107kD. Observed band size: 45kD
Lane 1: Mouse Hippocampus cell lysates probed with Eph receptor A7 Polyclonal Antibody, Unconjugated (bs-7034R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Rat Cerebrum cell lysates probed with Eph receptor A7 Polyclonal Antibody, Unconjugated (bs-7034R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-7034r

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-6318R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated