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Catalog #1: bsm-33385M-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Rat

Conjugate: Biotin

Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (ATG5) Monoclonal Antibody, Unconjugated (ascites of bsm-33385M 5D7) at 1:2000 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.
Lane 1: Human Hela cell lysates; Lane 2: Human Panc-1 cell lysates; Lane 3: Human U87MG cell lysates; Lane 4: Human MCF-7 cell lysates; Lane 5: Human K562 cell lysates; Lane 6: Human SH-SY5Y cell lysates probed with ATG5\/APG5L Monoclonal Antibody, Unconjugated (bsm-33385M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (ATG5\/APG5L) monoclonal Antibody, Unconjugated (bsm-33385M) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (XXX) polyclonal Antibody, Unconjugated (bs-0061R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.) polyclonal Antibody, Unconjugated (bsm-33385M) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bsm-33385m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bsm-33297m-hrp

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-33297m-biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-42001m-hrp

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

(Negative control)Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated(bsm-42001M-Bio) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0437P-HRP and DAB (C-0010) staining.
(Negative control)Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated(bsm-42001M-Bio) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0437P-HRP and DAB (C-0010) staining.
(Negative control)Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated(bsm-42001M-Bio) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0437P-HRP and DAB (C-0010) staining.
(Negative control)Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated(bsm-42001M-Bio) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0437P-HRP and DAB (C-0010) staining.

Catalog #1: bsm-42001m-biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-41516M

Applications: WB

Reactivity: Mouse, SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41637M

Applications: ELISA

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41630M

Applications: WB, ELISA

Reactivity: Mouse, Virus, SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41615M

Applications: WB, ELISA

Reactivity: Mouse, SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41632M

Applications: ELISA

Reactivity: Virus

Conjugate: Unconjugated

Catalog #1: bsm-41634M

Applications: ELISA

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41635M

Applications: ELISA

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41631M

Applications: ELISA

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41627M

Applications: ELISA

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41636M

Applications: ELISA

Reactivity: Virus

Conjugate: Unconjugated

Catalog #1: bsm-41621M

Applications: ELISA

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41476M

Applications: WB, ELISA

Reactivity: Mouse, Virus, SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-60088m

Applications: WB, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: room temperature 2h\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: Arabidopsis thaliana\r\nProtein loading quantity: 5 \u03bcg\r\nExposure time: 10 s\r\nPredicted MW: 55 kDa\r\nObserved MW: 55 kDa
Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: room temperature 2h\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: Rice\r\nProtein loading quantity: 0.008 \u03bcg\r\nExposure time: 10 s\r\nPredicted MW: 55 kDa\r\nObserved MW: 55 kDa

Catalog #1: bsm-60435m

Applications: WB

Reactivity: Arab

Conjugate: Unconjugated

Catalog #1: bsm-60569m

Applications: WB, ICC/IF

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-60172m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:2000\r\nPrimary Ab incubation condition: 2 hours at room temperature\r\nSecondary Ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: MCF-7, BRL, C2C12, COS-7, N2a\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 17 kDa\r\nObserved MW: 17 kDa
Tissue: Human neuroblastoma\r\nSection type: Formalin-fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:200\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nSecondary Ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60140M

Catalog #1: bsm-60140m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:1000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nLysate: Jurkat\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 180 s\r\nPredicted MW: 152 kDa\r\nObserved MW: 170 kDa
Tissue: Human colon\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60267M
Tissue: Human colon cancer\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60267M

Catalog #1: bsm-60267m

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60330m

Applications: Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60416m

Applications: Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60420m

Applications: Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60421m

Applications: Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60424m

Applications: Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60418m

Applications: Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60477m

Applications: Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60476m

Applications: Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60628M

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60537m

Applications: Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60583m

Applications: Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60283M

Applications: FCM

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60272M

Applications: FCM

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60399M

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60410M

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60411M

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60392M

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60395M

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60408M

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60412M

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60414M

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60475M

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60500M

Applications: FCM

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60548M

Applications: FCM

Reactivity:

Conjugate: Unconjugated