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Catalog #1: bsm-60498M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60533M

Applications: WB

Reactivity:

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:1000\r\nPrimary ab incubation condition: room temperature 2h\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: HeLa, 293\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 60 kDa\r\nObserved MW: 60 kDa
Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:1000\r\nPrimary ab incubation condition: room temperature 2h\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: 4T1, Mouse brain, F9, Rat brain\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 60 kDa\r\nObserved MW: 60 kDa

Catalog #1: bsm-60541M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 4\u00b0C overnight\r\nSecondary Ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate:1: HeLa, 2: HEK-293, 3:4T1, 4: Raw264.7\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 10 s\r\nPredicted MW: 75 kDa\r\nObserved MW: 75 kDa
Tissue: Human breast cancer\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nSecondary Ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60404M
Tissue: Rat liver\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nSecondary Ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60404M

Catalog #1: bsm-60404m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:1000\r\nPrimary ab incubation condition: 4\u00b0C overnight\r\nLysate: HeLa, MCF-7, 3T3, Jurkat\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 56 kDa\r\nObserved MW: 54 kDa
Tissue: Human colon cancer\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60335M
Tissue: Mouse liver\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60335M
Tissue: Rat kidney\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60335M

Catalog #1: bsm-60335m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:5000\r\nPrimary Ab incubation condition: 4\u00b0C overnight\r\nSecondary Ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: 1: HeLa, 2: Jurkat, 3: HepG2, 4: NIH\/3T3\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 45 kDa\r\nObserved MW: 43 kDa
Tissue: Mouse brain\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nSecondary Ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60381M
Tissue: rat brain\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nSecondary Ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60381M
Tissue: Human spleen\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nSecondary Ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60381M

Catalog #1: bsm-60381m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 4\u00b0C overnight \r\nSecondary Ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: 1: HeLa, 2: HEK-293, 3: 4T1, 4: Raw264.7\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 21 kDa\r\nObserved MW: 21 kDa
Tissue: Rat colon\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nSecondary Ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60402M
Tissue: Mouse kidney\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nSecondary Ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60402M
Tissue: Human liver cancer\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nSecondary Ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60402M

Catalog #1: bsm-60402m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-60516m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:1000\r\nPrimary ab incubation condition: 4\u00b0C overnight\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: HeLa, 293, MCF-7, Raw264.7\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 127 kDa\r\nObserved MW: 127 kDa
Tissue: Rat colon\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60409M
Tissue: Mouse liver\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60409M
Tissue:Human liver cancer\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60409M

Catalog #1: bsm-60409m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:5000\r\nPrimary ab incubation condition: 4\u00b0C overnight\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate:1:Raji, 2: HepG2, 3: 4T1\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 10 s\r\nPredicted MW: 62 kDa\r\nObserved MW: 62 kDa
Tissue: Rat liver\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60419M
Tissue: Mouse brain\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60419M
Tissue: Human testis\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60419M

Catalog #1: bsm-60419m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:5000\r\nPrimary ab incubation condition: room temperature 2h\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: Human serum\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 10 s\r\nPredicted MW: 34-36 kDa\r\nObserved MW: 34-36 kDa
Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:1000\r\nPrimary ab incubation condition: room temperature 2h\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: Mouse serum\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 10 s\r\nPredicted MW: 34-36 kDa\r\nObserved MW: 34-36 kDa
Tissue: Mouse brain\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60544M
Tissue: human brain\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60544M

Catalog #1: bsm-60544m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:1000\r\nPrimary ab incubation condition: room temperature 2h\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: HeLa, 3T3, A431, N2a\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 27 kDa\r\nObserved MW: 27 kDa\r\n
Tissue: Mouse brain\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab:SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60584M
Tissue: human brain\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab:SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60584M

Catalog #1: bsm-60584m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition:4\u00b0C overnight\r\nLysate: 1: LnCap, 2: K562, 3: NIH\/3T3, 4: BRL\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 85 kDa\r\nObserved MW: 85 kDa
Tissue: Mouse colon\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60360M
Tissue: human colon\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60360M

Catalog #1: bsm-60360m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 4\u00b0C overnight\r\nLysate: 1: HeLa, 2: HEK-293, 3: HCT-116, 4: RAW264.7\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 61 kDa\r\nObserved MW: 61 kDa
Tissue: Human brain\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60333M
Tissue: Human breast cancer\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60333M

Catalog #1: bsm-60333m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1\uff1a1000\r\nPrimary ab incubation condition: overnight, at 4 degree\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: HeLa, 293, 4T1, Raw264.7\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 29 kDa\r\nObserved MW: 29 kDa
Tissue: Human liver\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60396M

Catalog #1: bsm-60396m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:1000\r\nPrimary ab incubation condition: 4 \u00b0C overnight\r\nLysate: HeLa, MCF-7, 293, 3T3\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 22 kDa\r\nObserved MW: 22 kDa
Tissue: Human colon cancer\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60355M

Catalog #1: bsm-60355m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:1000\r\nPrimary ab incubation condition: 4\u00b0C overnight\r\nLysate: HeLa, C2C12\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 120 kDa\r\nObserved MW: 130 kDa
Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:5000\r\nPrimary ab incubation condition: 4\u00b0C overnight\r\nLysate: A549\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 120 kDa\r\nObserved MW: 130 kDa
Tissue: Human lung adenocarcinoma\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60363M

Catalog #1: bsm-60363m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:1000\r\nPrimary ab incubation condition: 4\u00b0C overnight\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: HeLa, HepG2, A549, EL4.IL-2\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 40 kDa\r\nObserved MW: 35 kDa
Tissue: Human breast cancer\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60391M

Catalog #1: bsm-60391m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-60398m

Applications: WB, ICC/IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-60415m

Applications: WB, IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-60451m

Applications: WB, IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-60495m

Applications: WB, ICC/IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-60535m

Applications: WB, ICC/IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-60538m

Applications: WB, ICC/IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-60341M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60344M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60379M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60521m

Applications: WB, ICC/IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:1000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate: HeLa, Raji, Jurkat\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 1 s\r\nPredicted MW: 23 kDa\r\nObserved MW: 23 kDa
Tissue: Rat colon\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60486M
Tissue:Human lung squamous cancer\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60486M

Catalog #1: bsm-60486m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Rat

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 2 hours at room temperature\r\nSecondary Ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: 1: MCF-7\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 14 kDa\r\nObserved MW: 14 kDa
Tissue: Human breast cancer\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nSecondary Ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60394M

Catalog #1: bsm-60394m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:5000\r\nPrimary ab incubation condition: room temperature 2h\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: Human platelet, Raji\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 1 s\r\nPredicted MW: 40 kDa\r\nObserved MW: 47 kDa
Tissue: Human colon cancer\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60467M

Catalog #1: bsm-60467m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:5000\r\nPrimary ab incubation condition: room temperature 2h\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: 1: HeLa, 2: 293\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 38 kDa\r\nObserved MW: 38 kDa
Tissue: Human colon\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60469M
Tissue: Human-lung-squamous-carcinoma\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60469M

Catalog #1: bsm-60469m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60515m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60517m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Tissue: Human kidney\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:200\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60259M
Tissue: Human colon\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:200\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60259M
Tissue: Human colon cancer\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:200\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60259M

Catalog #1: bsm-60259m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60253m

Applications: WB, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60309m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60311m

Applications: WB, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60285m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60268m

Applications: WB, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60275m

Applications: WB, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60315m

Applications: WB, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60317m

Applications: WB, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60325m

Applications: WB, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60336m

Applications: WB, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60337m

Applications: WB, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 4\u00b0C overnight\r\nLysate: 1: HeLa, 2: HEK-293, 3: MCF-7, 4: Jurkat\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 56 kDa\r\nObserved MW: 56 kDa
Tissue: Human breast cancer\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60338M

Catalog #1: bsm-60338m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60343m

Applications: WB, ICC/IF

Reactivity: Human

Conjugate: Unconjugated