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Catalog #1: bsm-33400M-Cy5

Applications: WB, IF(IHC-P), IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-33400M-Cy3

Applications: WB, IF(IHC-P), IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-33400M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33400M-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33301m-fitc

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-33301m-cy7

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-33301m-biotin

Applications: IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-33301m-cy5

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-33301m-cy3

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-33301m-Cy5.5

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-33301m-hrp

Applications: IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-33268M-FITC

Applications: IF(ICC), IF

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-33268M-Cy7

Applications: IF(ICC), IF

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-33264M-Cy3

Applications: IF(ICC), IF

Reactivity: Human, Rat

Conjugate: Cy3

Catalog #1: bsm-33268M-Cy5.5

Applications: IF(ICC), IF

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-33268M-Cy5

Applications: IF(ICC), IF

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-33268M-Cy3

Applications: IF(ICC), IF

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-33268M-HRP

Applications: IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-33268M-Biotin

Applications: IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with \r\nJAK1 Monoclonal Antibody, Unconjugated (bsm-33268M) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with \r\nJAK1 Monoclonal Antibody, Unconjugated (bsm-33268M) at 1:400 overnight at 4\u00b0C, DAB staining.
MCF-7 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with JAK1 Monoclonal Antibody(bsm-33267M)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bsm-33268M

Applications: IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33264M-FITC

Applications: IF(ICC), IF

Reactivity: Human, Rat

Conjugate: FITC

Catalog #1: bsm-33264M-Cy7

Applications: IF(ICC), IF

Reactivity: Human, Rat

Conjugate: Cy7

Catalog #1: bsm-33264M-Cy5.5

Applications: IF(ICC), IF

Reactivity: Human, Rat

Conjugate: Cy5.5

Catalog #1: bsm-33264M-Cy5

Applications: IF(ICC), IF

Reactivity: Human, Rat

Conjugate: Cy5

Catalog #1: bsm-33264M-HRP

Applications: IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: HRP

Catalog #1: bsm-33264M-Biotin

Applications: IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: Biotin

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RPS6KB1 Monoclonal Antibody, Unconjugated (bsm-33264M) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RPS6KB1 Monoclonal Antibody, Unconjugated (bsm-33264M) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RPS6KB1 Monoclonal Antibody, Unconjugated (bsm-33264M) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-33264M

Applications: IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33234m-fitc

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-33234m-cy7

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-33234m-Cy5.5

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-33234m-cy5

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-33234m-cy3

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-33234m-hrp

Applications: IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33234m-biotin

Applications: IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ERK1\/2 Monoclonal Antibody, Unconjugated (bsm-33234M) at 1:2000 overnight at 4\u00b0C, DAB staining.
4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (ERK1\/2) monoclonal Antibody, unconjugated (bsm-33234M) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-ERK1\/2 antibody (bsm-33234M): 1 \u03bcg\/10^6 cells; Isotype Control (orange): Mouse IgG (bs-0296P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bsm-33234m

Applications: FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33232m-fitc

Applications: WB, FCM, IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-33232m-cy7

Applications: WB, FCM, IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-33232m-Cy5.5

Applications: WB, FCM, IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-33232m-cy3

Applications: WB, FCM, IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-33232m-cy5

Applications: WB, FCM, IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-33232m-hrp

Applications: WB, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33232m-biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: Biotin

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ERK1\/2 Monoclonal Antibody, Unconjugated (bsm-33232M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ERK1\/2 Monoclonal Antibody, Unconjugated (bsm-33232M) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates probed with ERK1\/2 Monoclonal Antibody, Unconjugated (bsm-33232M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-ERK1\/2 antibody (bsm-33232M): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-BF488 (bs-60296G-BF488): 1 \u03bcg\/test. Isotype Control (orange): Mouse IgG (bs-0296P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bsm-33232m

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33209M-Cy7

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-33209M-Cy5.5

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-33209M-Cy3

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-33209M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33207m-hrp

Applications: WB

Reactivity: Rat

Conjugate: HRP