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Lane 1: Recombinant human FOXP3 protein, N-His(bs-42173P) probed with FOXP3 Monoclonal Antibody, Unconjugated (bsm-42173M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.
The MJ (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-FOXP3 antibody (bsm-42173M): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-FITC (bs-60296G-FITC): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bsm-42173m

Applications: FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-42173m-bf350

Applications: FCM

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-350

Catalog #1: bsm-42106m-percp-cy7

Applications: WB

Reactivity: Human

Conjugate: PerCP-Cy7

Catalog #1: bsm-42050m-percp-cy7

Applications: WB, IF(ICC)

Reactivity: Human

Conjugate: PerCP-Cy7

Catalog #1: bsm-42005m-percp-cy7

Applications: WB, IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: PerCP-Cy7

Catalog #1: bsm-41804m-rbitc

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: RBITC

Catalog #1: bsm-41804m-percp-Cy5.5

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-41804m-cy7

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-41804m-pe

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-41804m-Cy5.5

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-41804m-percp

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: PerCP

Catalog #1: bsm-41804m-hrp

Applications: IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-41804m-fitc

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-41804m-bf750

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-750

Catalog #1: bsm-41802m-pe-cy7

Applications: WB

Reactivity: Human

Conjugate: PE-Cy7

Catalog #1: bsm-41804m-cy3

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-41804m-bf405

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bsm-41804m-bf680

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-680

Catalog #1: bsm-41804m-biotin

Applications: IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-41804m-cy5

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-41804m-bf647

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bsm-41804m-bf594

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bsm-41804m-apc-Cy5.5

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bsm-41804m-bf488

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bsm-41804m-bf555

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bsm-41804m-bf350

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bsm-41802m-percp-cy7

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: PerCP-Cy7

Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated(bsm-41804M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated(bsm-41804M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated(bsm-41804M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated(bsm-41804M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining DAB staining.

Catalog #1: bsm-41804m

Applications: IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-41802m-rbitc

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: RBITC

Catalog #1: bsm-41802m-cy7

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-41804m-apc

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: APC

Catalog #1: bsm-41802m-percp

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: PerCP

Catalog #1: bsm-41802m-pe

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-41802m-Cy5.5

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-41802m-pe-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5.5

Catalog #1: bsm-41802m-percp-Cy5.5

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-41802m-pe-cy5

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5

Catalog #1: bsm-41802m-fitc

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-41802m-cy3

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: Cy3

Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated(bsm-41802M) at 1:200overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated(bsm-41802M) at 1:200overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated(bsm-41802M) at 1:200overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated(bsm-41802M) at 1:200overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining DAB staining.

Catalog #1: bsm-41802m

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-41802m-hrp

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-41802m-apc-cy7

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: APC-Cy7

Catalog #1: bsm-41802m-biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-41802m-bf488

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bsm-41802m-bf750

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-750

Catalog #1: bsm-41802m-cy5

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-41802m-bf594

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bsm-41802m-bf647

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-647