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Paraformaldehyde-fixed, paraffin embedded (Human melanoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD63) Monoclonal Antibody, Unconjugated (bsm-34138M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.\r\n\r\n
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD63) Monoclonal Antibody, Unconjugated (bsm-34138M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.\r\n\r\n
25 ug total protein per lane of various lysates (see on figure) probed with CD63 monoclonal antibody, unconjugated (bsm-34138M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-34138m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-34118m-percp

Applications: IF

Reactivity: Species independent

Conjugate: PerCP

Catalog #1: bsm-34118m-pe

Applications: IF

Reactivity: Species independent

Conjugate: PE

Catalog #1: bsm-34118m-fitc

Applications: IF

Reactivity: Species independent

Conjugate: FITC

Catalog #1: bsm-34118m-hrp

Applications: ELISA, IHC-P, IHC-F

Reactivity: Species independent

Conjugate: HRP

Catalog #1: bsm-34118m-rbitc

Applications: IF

Reactivity: Species independent

Conjugate: RBITC

Catalog #1: bsm-34118m-percp-Cy5.5

Applications: IF

Reactivity: Species independent

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-34118m-Cy5.5

Applications: IF

Reactivity: Species independent

Conjugate: Cy5.5

Catalog #1: bsm-34118m-cy7

Applications: IF

Reactivity: Species independent

Conjugate: Cy7

Catalog #1: bsm-34118m-cy5

Applications: IF

Reactivity: Species independent

Conjugate: Cy5

Catalog #1: bsm-34118m-bf680

Applications: IF

Reactivity: Species independent

Conjugate: AbBy Fluor-680

Catalog #1: bsm-34118m-bf555

Applications: IF

Reactivity: Species independent

Conjugate: AbBy Fluor-555

Catalog #1: bsm-34118m-bf594

Applications: IF

Reactivity: Species independent

Conjugate: AbBy Fluor-594

Catalog #1: bsm-34118m-cy3

Applications: IF

Reactivity: Species independent

Conjugate: Cy3

Catalog #1: bsm-34118m-bf750

Applications: IF

Reactivity: Species independent

Conjugate: AbBy Fluor-750

Catalog #1: bsm-34118m-biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Species independent

Conjugate: Biotin

Catalog #1: bsm-34118m-bf488

Applications: IF

Reactivity: Species independent

Conjugate: AbBy Fluor-488

Catalog #1: bsm-34118m-bf350

Applications: IF

Reactivity: Species independent

Conjugate: AbBy Fluor-350

Catalog #1: bsm-34118m-apc

Applications: IF

Reactivity: Species independent

Conjugate: APC

Catalog #1: bsm-34118m-bf405

Applications: IF

Reactivity: Species independent

Conjugate: AbBy Fluor-405

Catalog #1: bsm-34016r-percp-cy7

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy7

Catalog #1: bsm-34118m-apc-Cy5.5

Applications: IF

Reactivity: Species independent

Conjugate: APC-Cy5.5

Catalog #1: bsm-33895m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33171m-percp

Applications: WB

Reactivity: Human

Conjugate: PerCP

Catalog #1: bsm-33171m-rbitc

Applications: WB

Reactivity: Human

Conjugate: RBITC

Catalog #1: bsm-33171m-hrp

Applications: WB

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-33171m-pe-cy7

Applications: WB

Reactivity: Human

Conjugate: PE-Cy7

Catalog #1: bsm-33171m-percp-cy7

Applications: WB

Reactivity: Human

Conjugate: PerCP-Cy7

Catalog #1: bsm-33171m-pe

Applications: WB

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-33171m-pe-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5.5

Catalog #1: bsm-33171m-cy7

Applications: WB

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-33171m-percp-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-33171m-fitc

Applications: WB

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-33171m-cy3

Applications: WB

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-33171m-biotin

Applications: WB

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-33171m-cy5

Applications: WB

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-33171m-pe-cy5

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5

Catalog #1: bsm-33171m-bf750

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-750

Catalog #1: bsm-33171m-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-33171m-bf680

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-680

Catalog #1: bsm-33171m-bf594

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bsm-33171m-bf555

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bsm-33171m-bf405

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33171m-bf488

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33171m-bf647

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bsm-33171m-bf350

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33171m-apc-cy7

Applications: WB

Reactivity: Human

Conjugate: APC-Cy7

Catalog #1: bsm-33171m-apc-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: APC-Cy5.5