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Catalog #1: bs-5977R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Hela cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with \r\nFerritin heavy chain Polyclonal Antibody(bs-5907R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Ferritin heavy chain Polyclonal Antibody, Unconjugated (bs-5907R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Ferritin heavy chain Polyclonal Antibody, Unconjugated (bs-5907R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Ferritin heavy chain Polyclonal Antibody, Unconjugated (bs-5907R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-5907R

Applications: WB, ELISA, FCM, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-3922R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-SCD1 Polyclonal Antibody, Unconjugated (bs-3787R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with SCD1 Polyclonal Antibody (bs-3787R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SCD1 Polyclonal Antibody, Unconjugated (bs-3787R) at 1:400 overnight at 4\u00b0C, DAB staining.
A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (SCD1) polyclonal Antibody, Unconjugated (bs-3787R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-3787R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Rat brain and heart lysates probed with CD1d1 Polyclonal Antibody, unconjugated (bs-3690R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:3000 for 90 minutes at 37\u00b0C.\\n
Mouse spleen cells probed with \\tCD1d1 Polyclonal Antibody, Unconjugated (bs-3690R) at 1:100 for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Catalog #1: bs-3690R

Applications: WB, FCM, IHC-P, IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Lane 1: mouse liver lysates Lane 2: mouse intestine lysates probed with Anti AHSG\/Fetuin A\/Alpha 2 HS Glycoprotein Polyclonal Antibody, Unconjugated (bs-2922R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 31kD. Observed band size: 31kD.
Antigen: bs-2922P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Lane 1: Mouse Liver lysates probed with AHSG Polyclonal Antibody, Unconjugated (bs-2922R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2922R

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human brain labeled with Anti-HDAC8 Polyclonal Antibody, Unconjugated (bs-2891R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse pancreas labeled with Anti-HDAC8 Polyclonal Antibody, Unconjugated (bs-2891R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Catalog #1: bs-2891R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded rat lung tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-IRF7 Polyclonal Antibody, Unconjugated (bs-2994R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-IRF7 Polyclonal Antibody, Unconjugated (bs-2994R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Mouse spleen cells were fixed with 4% PFA for 10min at room temperature,permeabilized with ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IRF7 Antibody(bs-2994R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-2994R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse spleen and bone lysates probed with Rabbit Anti-SIRP Alpha Polyclonal Antibody, Unconjugated (bs-2708R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
Human U937 probed with \\tSIRP Alpha Polyclonal Antibody (bs-2708R) at 1:100 for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Lane 1: Mouse Cerebrum lysates probed with SIRP Alpha Polyclonal Antibody, Unconjugated (bs-2708R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Brain Lysates; Lane 2: Mouse Cerebellum Lysates; Lane 3: Mouse Spleen Lysates; Lane 4: Mouse Placenta Lysates; Lane 5: Rat Brain Lysates; Lane 6: Rat Cerebellum Lysates. Probed with SIRP Alpha polyclonal Antibody, unconjugated (bs-2708R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2708R

Applications: WB, ELISA, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human pancreatic cancer labeled with Anti-EDG2\/LPA1 Polyclonal Antibody, Unconjugated (bs-2880R) at 1:400 followed by conjugation to the secondary antibody and DAB staining
L1 Mouse liver lysates, L2 Mouse cerebrum lysates probed with Anti- EDG2\/LPA1 Polyclonal Antibody, Unconjugated (bs-2880R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:2000 90min in 37˚C. Predicted band and observed band size: 41kD
Lane 1: Mouse Heart lysates probed with LPAR1 Polyclonal Antibody, Unconjugated (bs-2880R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Kidney tissue lysates; Lane 2: Rat Kidney tissue lysates probed with LPA1\/LPAR1 Polyclonal Antibody, Unconjugated (bs-2880R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2880R

Applications: WB, ELISA, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse heart lysates probed with SPHK1 Polyclonal Antibody, unconjugated (bs-2652R) at 1:30 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.\\n
Lane 1: Mouse Pancreas tissue lysates; Lane 2: Mouse Lung tissue lysates; Lane 3: Rat Kidney tissue lysates probed with SPHK1 Polyclonal Antibody, Unconjugated (bs-2652R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2652R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti phospho-Bim(Ser87) Polyclonal Antibody, Unconjugated (bs-3012R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti-Bim(Ser87) Polyclonal Antibody, Unconjugated (bs-3012R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Raw264.7 cell lysates probed with Bim(Ser87) Polyclonal Antibody, Unconjugated (bs-3012R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3012R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2905R

Applications: ELISA, IHC-P, IF(IHC-P)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-2453R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Rat splenocytes stained with Anti- TLR6\/CD286 Polyclonal Antibody, PE-CY5.5 Conjugated (bs-2716R-PE-Cy5.5) at 1:25.
Rat splenocytes stained with Anti- TLR6\/CD286 Polyclonal Antibody, PE-CY5.5 Conjugated (bs-2716R-PE-Cy5.5) at 1:25
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-TLR6 Polyclonal Antibody, Unconjugated (bs-2716R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse splenocytes probed with TLR6 Polyclonal Antibody, Unconjugated (bs-2716R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Catalog #1: bs-2716R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Bdkrb2\/B2R Polyclonal Antibody, Unconjugated (bs-2422R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Mouse brain cells probed with Rabbit Anti-Bdkrb2 Polyclonal Antibody (bs-2422R) at 1:50 for 40 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary (bs-0295G-FITC) at 1:100 (green) for 40 minutes compared to control cells (blue).

Catalog #1: bs-2422R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human osteosarcoma tissue labeled with Anti-Twist Polyclonal Antibody (bs-2441R), Unconjugated at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining
Paraformaldehyde-fixed, paraffin embedded Rat placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TWIST1\/2 Polyclonal Antibody, Unconjugated (bs-2441R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-2441R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2411R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Human U937 cells probed with Integrin alpha 1 Polyclonal Antibody, Unconjugated (bs-2095R) at 0.2ug for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Lane 1: Human Hela cell lysates; Lane 2: Human 293T cell lysates; Lane 3: Human U-87MG cell lysates; Lane 4: Human HepG2 cell lysates; Lane 5: Human A549 cell lysates probed with Integrin alpha 1 Polyclonal Antibody, Unconjugated (bs-2095R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2095r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti- 5-HTR3Polyclonal Antibody, Unconjugated(bs-2126R) followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Cerebellum lysates; Lane 2: Mouse Cerebrum lysates; Lane 3: Mouse Spinal cord lysates; Lane 4: Mouse Heart lysates ; Lane 5: Rat Heart lysates probed with 5-HTR3\/HTR3A Polyclonal Antibody, Unconjugated (bs-2126R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2126R

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2047R

Applications: WB, ELISA, IHC-P

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Lane 1:rat heart lysates Lane 2: rat brain lysates probed with Anti A2AR\/Adenosine A2a receptor Polyclonal Antibody, Unconjugated (bs-1456R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 64kD. Observed band size: 64kD.
Formalin-fixed and paraffin embedded mouse brain labeled with Anti-A2AR\/Adenosine A2a Polyclonal Antibody, Unconjugated (bs-3927R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-1456R

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti-FCGBP Polyclonal Antibody, Unconjugated (bs-13168R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Paraformaldehyde-fixed, paraffin embedded Mouse intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FCGBP Polyclonal Antibody, Unconjugated (bs-13168R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FCGBP Polyclonal Antibody, Unconjugated (bs-13168R) at 1:200 overnight at 4\u00b0C, DAB staining.
Positive sample\uff1aParaformaldehyde-fixed, paraffin embedded (rat intestine, left); Negative sample\uff1aParaformaldehyde-fixed, paraffin embedded (rat lung, right);\r\nAntigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (FCGBP) Polyclonal Antibody, Unconjugated (bs-13168R) at 1:200 overnight at 4\u00b0C, followed by secondary antibody and DAB staining.\r\n

Catalog #1: bs-13168R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Human K562 cells and mouse heart lysates probed with Polyclonal Antibody, unconjugated (bs-11848R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.\r\n
A549 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with GPR56 Polyclonal Antibody (bs-11848R)at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-11848R

Applications: WB, ELISA, FCM

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-TMEM158 Polyclonal Antibody, Unconjugated (bs-11770R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TMEM158 Polyclonal Antibody, Unconjugated (bs-11770R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TMEM158 Polyclonal Antibody, Unconjugated (bs-11770R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAPI staining

Catalog #1: bs-11770R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti- Scavenger receptor class B member 1 Polyclonal Antibody, Unconjugated (bs-1186R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Image kindly submitted by Brittany Baldwin-Murphy from Cornell University.\\n\\nChick embryo liver hepatocytes probed with Rabbit Anti- Scavenger receptor class B member 1 Polyclonal Antibody at 1:100 or 1:500.\\n
Mouse liver cells probed with Rabbit Anti- Scavenger receptor class B member 1 Polyclonal Antibody (bs-1186R) at 1:100 for 60 minutes followed by incubation with Goat Anti-Rabbit IgG PE conjugated secondary (bs-0295G-PE) at 1:100 (green) for 40 minutes compared to control cells (blue).
HepG2 lysates probed with Scavenger receptor class B member 1 Polyclonal Antibody, unconjugated (bs-1186R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.

Catalog #1: bs-1186R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-1173R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-11514R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human laryngocarcinoma labeled with Anti-SPRR3\/Cornifin B Polyclonal Antibody, Unconjugated (bs-11163R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
U937 cell lysates probed with SPRR3 Polyclonal Antibody, Unconjugated (bs-11163R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-11163R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse stomach labeled with Anti-SGCB\/SGC Polyclonal Antibody, Unconjugated (bs-1127R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat pancreas labeled with Anti-SGCB\/SGC Polyclonal Antibody, Unconjugated (bs-1127R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Catalog #1: bs-1127R

Applications: IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Nox4\/NADH Polyclonal Antibody, Unconjugated (bs-1091R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Mouse muscle lysates probed with Rabbit Anti-NADPH oxidase 4 Polyclonal Antibody, Unconjugated (bs-1091R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
Lane 1: Human HUVEC cell lysates; Lane 2: Human 293T cell lysates; Lane 3: Human U251 cell lysates probed with NADPH oxidase 4 Polyclonal Antibody, Unconjugated (bs-1091R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
293T cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with NADPH oxidase 4 Antibody(bs-1091R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-1091R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse kidney tissue labeled with Rabbit Anti-L-Selectin\/CD62L Polyclonal Antibody (bs-1036R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse kidney tissue labeled with Rabbit Anti-L-Selectin\/CD62L Polyclonal Antibody (bs-1036R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat colitis tissue labeled with Rabbit Anti-L-Selectin\/CD62L Polyclonal Antibody (bs-1036R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 rat heart lysates L2 rat brain lysates probed with Anti L-Selectin\/CD62L Polyclonal Antibody, Unconjugated (bs-1036R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 38kD. Observed band size:38kD.\\n

Catalog #1: bs-1036R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat spinal cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Alpha-Synuclein Polyclonal Antibody, Unconjugated (bs-0968R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Alpha-Synuclein Polyclonal Antibody, Unconjugated (bs-0968R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Alpha-Synuclein Polyclonal Antibody, Unconjugated (bs-0968R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Alpha-Synuclein Polyclonal Antibody, Unconjugated (bs-0968R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0968r

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0961R-PE

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bs-0813R-HRP

Applications: WB, ELISA, IHC-F

Reactivity: Bovine, Cow

Conjugate: HRP

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-Cyclin B1 Polyclonal Antibody, Unconjugated (bs-0572R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
HeLa cells probed with Rabbit Anti-Cyclin B1 Polyclonal Antibody (bs-0572R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated Secondary.\\n
HeLa lysates probed with Anti \u2013(Name) Polyclonal Antibody, Unconjugated (Catalog #) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (Secondary Catalog #) at 1:5000 for 90 min at 37˚C.
U937 lysates probed with Anti \u2013(Name) Polyclonal Antibody, Unconjugated (Catalog #) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (Secondary Catalog #) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-0572R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Mouse kidney lysates probed with Anti MUC13 Polyclonal Antibody, Unconjugated (bs-10074R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (MUC13) Polyclonal Antibody, Unconjugated (bs-10074R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-10074R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human endometrium tissue labeled with Anti-ER-alpha\/beta Polyclonal Antibody, Unconjugated (bs-0174R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 rat brain, L2 rat colon lysates probed (bs-0174R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band and observed band size: 58kDa.
Tissue\/cell: HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Estrogen Receptor alpha + beta ) Polyclonal Antibody, Unconjugated (bs-0174R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Estrogen Receptor alpha + beta Polyclonal Antibody, Unconjugated (bs-0174R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0174R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-Rat IgG Polyclonal Antibody, Unconjugated (bs-0293R) at 1:600, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-Rat IgG Polyclonal Antibody, FITC conjugated (bs-0293R-FITC) at 1:200, followed by DAPI staining
Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-Rat IgG Polyclonal Antibody, FITC conjugated (bs-0293R-FITC) at 1:200

Catalog #1: bs-0293R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Lane 1: MCF-7 Lane 2: DU145 lysates probed with Anti-Estrogen Receptor alpha Polyclonal Antibody, Unconjugated (bs-0122R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.
Tissue\/cell:MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Estrogen Receptor alpha) polyclonal Antibody, Unconjugated (bs-0122R) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: Human MCF-7 cell lysates; Lane 2: Human K562 cell lysates; Lane 3: Rat Liver lysates; Lane 4: Human MOLT-4 cell lysates; Lane 5: Human Raji cell lysates; Lane 6: Human Hela cell lysates; Lane 7: Human A549 cell lysates probed with Estrogen Receptor alpha Polyclonal Antibody, Unconjugated (bs-0122R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0122R

Applications: WB, ELISA, FCM, IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Ki-67 Polyclonal Antibody, Unconjugated (bs-23103R) at 1:400 overnight at 4\u00b0C, DAB staining.
Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Ki-67 Polyclonal Antibody(bs-23103R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-23103R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MMP-1 Polyclonal Antibody, Unconjugated (bs-4597R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MMP-1 Polyclonal Antibody, Unconjugated (bs-4597R) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Rat Testis lysates; Lane 2: Mouse Testis lysates probed with MMP-1 Polyclonal Antibody, Unconjugated (bs-4597R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MMP-1 Polyclonal Antibody, Unconjugated (bs-4597R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-4597R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat liver tissue labeled with Anti Protective Protein Polyclonal Antibody, Unconjugated (bs-6040R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse testis lysates probed withRabbit Anti-Lysosomal Protective Protein Polyclonal Antibody, Unconjugated (bs-6040R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.
Human 293T lysates probed with Lysosomal Protective Protein Polyclonal Antibody, unconjugated (bs-6040R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.

Catalog #1: bs-6040R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-8688R

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti hospho-DDX5 (Tyr593) Polyclonal Antibody, Unconjugated (bs-8005R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse kidney labeled with Rabbit Anti hospho-DDX5 (Tyr593) Polyclonal Antibody, Unconjugated (bs-8005R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-8005R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-6692R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-6216R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human breast cancer labeled with Anti SIRT3 Polyclonal Antibody, Unconjugated (bs-6105R) followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Liver lysates; Lane 2: Mouse Kindey lysates; Lane 3: Mouse Cerebrum lysates; Lane 4: MouseHeart lysates probed with SIRT3 Polyclonal Antibody, Unconjugated (bs-6105R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (SIRT3) Polyclonal Antibody, Unconjugated (bs-6105R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (SIRT3) Polyclonal Antibody, Unconjugated (bs-6105R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-6105R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated