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Catalog #1: bsm-41664m-bf680

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-680

Catalog #1: bsm-41664m-bf555

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bsm-41664m-bf647

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bsm-41664m-bf488

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bsm-41664m-bf350

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bsm-41664m-bf405

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bsm-41664m-apc-cy7

Applications: WB

Reactivity: Human

Conjugate: APC-Cy7

Catalog #1: bsm-41664m-apc

Applications: WB

Reactivity: Human

Conjugate: APC

Catalog #1: bsm-41664m-apc-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bsm-41664m

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD57 Monoclonal Antibody, Unconjugated(bsm-60790M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Chronic tonsillitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD57 Monoclonal Antibody, Unconjugated(bsm-60790M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD57 Monoclonal Antibody, Unconjugated(bsm-60790M) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Prostate Tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD57 Monoclonal Antibody, Unconjugated(bsm-60790M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-60790m

Applications: IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (human spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (IFI16) Monoclonal Antibody, Unconjugated (bsm-60844M) at 1:300 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (IFI16) Monoclonal Antibody, Unconjugated (bsm-60844M) at 1:300 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (IFI16) Monoclonal Antibody, Unconjugated (bsm-60844M) at 1:300 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human tonsils; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IFI16 Monoclonal Antibody, Unconjugated (bsm-60844M) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Mouse IgG antibody (red, bs-0296G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bsm-60844m

Applications: IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33428m-pe-Cy5.5

Applications: WB, FCM

Reactivity: Human

Conjugate: PE-Cy5.5

Catalog #1: bsm-33428m-percp-cy7

Applications: WB, FCM, IF(ICC)

Reactivity: Human

Conjugate: PerCP-Cy7

Catalog #1: bsm-33428m-bf750

Applications: WB, FCM, IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-750

Catalog #1: bsm-33428m-bf555

Applications: WB, FCM, IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bsm-33428m-bf680

Applications: WB, FCM, IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-680

Catalog #1: bsm-33428m-cy3

Applications: WB, FCM, IF(ICC)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-33428m-bf350

Applications: WB, FCM, IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33428m-pe-cy7

Applications: WB, FCM

Reactivity: Human

Conjugate: PE-Cy7

Catalog #1: bsm-33428m-fitc

Applications: WB, FCM, IF(ICC)

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-33428m-apc

Applications: WB, FCM, IF(ICC)

Reactivity: Human

Conjugate: APC

Catalog #1: bsm-33428m-percp

Applications: WB, FCM, IF(ICC)

Reactivity: Human

Conjugate: PerCP

Catalog #1: bsm-33428m-bf647

Applications: WB, FCM, IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bsm-33428m-bf488

Applications: WB, FCM, IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33428m-apc-Cy5.5

Applications: WB, FCM, IF(ICC)

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bsm-33428m-hrp

Applications: WB

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-33428m-bf594

Applications: WB, FCM, IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bsm-33428m-cy5

Applications: WB, FCM, IF(ICC)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-33428m-bf405

Applications: WB, FCM, IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33428m-biotin

Applications: WB

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-33428m-pe-cy5

Applications: WB, FCM

Reactivity: Human

Conjugate: PE-Cy5

Catalog #1: bsm-33428m-apc-cy7

Applications: WB, FCM, IF(ICC)

Reactivity: Human

Conjugate: APC-Cy7

Catalog #1: bsm-33428m-rbitc

Applications: WB, FCM, IF(ICC)

Reactivity: Human

Conjugate: RBITC

Catalog #1: bsm-33428m-cy7

Applications: WB, FCM, IF(ICC)

Reactivity: Human

Conjugate: Cy7

Hep-G2 (H) cells were treated with or without CoCl2 (500uM) for 6 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with HIF-1 Alpha monoclonal antibody, unconjugated (bsm-33428M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed HepG2\uff08treated with 1mM CoCl2 for 24 hours\uff09 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (HIF-1a) monoclonal Antibody, unconjugated (bsm-33428M) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
4% Paraformaldehyde-fixed Hela\uff08treated with 1mM CoCl2 for 24 hours\uff09 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (HIF-1a) monoclonal Antibody, unconjugated (bsm-33428M) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The Hela (treated with 500uM CoCl2 for 6 hours) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-HIF-1 Alpha antibody (bsm-33428M): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-BF488 (bs-60296G-BF488): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bsm-33428m

Applications: WB, FCM, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33428m-Cy5.5

Applications: WB, FCM, IF(ICC)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-33428m-percp-Cy5.5

Applications: WB, FCM, IF(ICC)

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-41201m-hrp

Applications: IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-41201m-biotin

Applications: IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD105Monoclonal Antibody, Unconjugated (ascites of bsm-41201M) at 1:1500 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD105Monoclonal Antibody, Unconjugated (ascites of bsm-41201M) at 1:1500 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD105Monoclonal Antibody, Unconjugated (ascites of bsm-41201M) at 1:1500 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD105Monoclonal Antibody, Unconjugated (ascites of bsm-41201M) at 1:1500 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-41201m

Applications: IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-55552m

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with NEFM monoclonal antibody, unconjugated (bsm-55531M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NEFM Monoclonal Antibody, Unconjugated(bsm-55531M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NEFM Monoclonal Antibody, Unconjugated(bsm-55531M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NEFM Monoclonal Antibody, Unconjugated(bsm-55531M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.

Catalog #1: bsm-55531m

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-35030m

Applications: Flow-Cyt

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33148m-percp-cy7

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: PerCP-Cy7

Catalog #1: bsm-33148m-fitc

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: FITC

Catalog #1: bsm-33148m-pe-Cy5.5

Applications: WB, FCM

Reactivity: Species independent

Conjugate: PE-Cy5.5

Catalog #1: bsm-33148m-percp-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: PerCP-Cy5.5