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Catalog #1: bsm-49133m-cy7

Applications: WB

Reactivity:

Conjugate: Cy7

Catalog #1: bsm-49133M-Cy5.5

Applications: WB

Reactivity:

Conjugate: Cy5.5

Catalog #1: bsm-49133m-cy5

Applications: WB

Reactivity:

Conjugate: Cy5

Catalog #1: bsm-49133m-cy3

Applications: WB

Reactivity:

Conjugate: Cy3

Catalog #1: bsm-49131m-rbitc

Applications: WB

Reactivity:

Conjugate: RBITC

Catalog #1: bsm-49131m-percp-cy5.5

Applications: WB

Reactivity:

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-49131m-percp

Applications: WB

Reactivity:

Conjugate: PerCP

Catalog #1: bsm-49131m-pe-cy7

Applications: WB

Reactivity:

Conjugate: PE-Cy7

Catalog #1: bsm-49131m-pe-cy5.5

Applications: WB

Reactivity:

Conjugate: PE-Cy5.5

Catalog #1: bsm-49131m-pe-cy5

Applications: WB

Reactivity:

Conjugate: PE-Cy5

Catalog #1: bsm-49131m-apc-cy7

Applications: WB

Reactivity:

Conjugate: APC-Cy7

Catalog #1: bsm-49131m-apc-cy5.5

Applications: WB

Reactivity:

Conjugate: APC-Cy5.5

Catalog #1: bsm-49131m-apc

Applications: WB

Reactivity:

Conjugate: APC

Catalog #1: bsm-49131m-bf750

Applications: WB

Reactivity:

Conjugate: AbBy Fluor-750

Catalog #1: bsm-49131m-bf680

Applications: WB

Reactivity:

Conjugate: AbBy Fluor-680

Catalog #1: bsm-49131m-bf647

Applications: WB

Reactivity:

Conjugate: AbBy Fluor-647

Catalog #1: bsm-49131m-bf594

Applications: WB

Reactivity:

Conjugate: AbBy Fluor-594

Catalog #1: bsm-49131m-bf555

Applications: WB

Reactivity:

Conjugate: AbBy Fluor-555

Catalog #1: bsm-49131m-bf488

Applications: WB

Reactivity:

Conjugate: AbBy Fluor-488

Catalog #1: bsm-49131m-bf405

Applications: WB

Reactivity:

Conjugate: AbBy Fluor-405

Catalog #1: bsm-49131m-bf350

Applications: WB

Reactivity:

Conjugate: AbBy Fluor-350

Catalog #1: bsm-49131m-pe

Applications: WB

Reactivity:

Conjugate: PE

Catalog #1: bsm-49131m-fitc

Applications: WB

Reactivity:

Conjugate: FITC

Catalog #1: bsm-49131m-cy7

Applications: WB

Reactivity:

Conjugate: Cy7

Catalog #1: bsm-49131m-cy5.5

Applications: WB

Reactivity:

Conjugate: Cy5.5

Catalog #1: bsm-49131m-cy5

Applications: WB

Reactivity:

Conjugate: Cy5

Catalog #1: bsm-49131m-cy3

Applications: WB

Reactivity:

Conjugate: Cy3

Catalog #1: bsm-30093M-PE

Applications: FCM

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-30093M-Cy7

Applications: FCM

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-30093M-Cy5.5

Applications: FCM

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-30093M-Cy5

Applications: FCM

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-30093M-Cy3

Applications: FCM

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-53023M

Applications: WB, ELISA, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51000R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51023R

Applications: WB, ELISA, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51013R

Applications: WB, ELISA, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51012R

Applications: WB, ELISA, IF

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EIF2S1 Monoclonal Antibody, Unconjugated (bsm-51644M) at 1:1000 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EIF2S1 Monoclonal Antibody, Unconjugated (bsm-51644M) at 1:1000 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EIF2S1 Monoclonal Antibody, Unconjugated (bsm-51644M) at 1:1000 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EIF2S1 Monoclonal Antibody, Unconjugated (bsm-51644M) at 1:1000 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-51644m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Calpain 2 Monoclonal Antibody, Unconjugated (bsm-51592M) at 1:25 overnight at 4\u00b0C, DAB staining.
Lane 1: A549 cell lysates; Lane 2: A431 cell lysates; Lane 3: SK-BR-3 cell lysates probed with Calpain 2 Monoclonal Antibody, Unconjugated (bsm-51592M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51592m

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-51504m

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Lane 1: Human Brain tissue lysates; Lane 2: Mouse Brain tissue lysates probed with CRMP2 Monoclonal Antibody, Unconjugated (bsm-51453M) at 1:8000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse NIH\/3T3 cell lysates; Lane 2: Mouse Brain tissue lysates probed with CRMP2 Monoclonal Antibody, Unconjugated (bsm-51453M) at 1:12800 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51453m

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-49131m-biotin

Applications: WB, ELISA

Reactivity:

Conjugate: Biotin

Catalog #1: bsm-49133m-biotin

Applications: WB, ELISA

Reactivity:

Conjugate: Biotin

Catalog #1: bsm-49135m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-49133m-hrp

Applications: WB, ELISA

Reactivity:

Conjugate: HRP

Catalog #1: bsm-49131m-hrp

Applications: WB, ELISA

Reactivity:

Conjugate: HRP

Histone extracts (15 \u03bcg) from HeLa cells were analysed by Western blot using H3K4un (3C5) Monoclonal Antibody (Cat. No. bsm-53012M) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
HeLa cells were stained with H3K4un (3C5) Monoclonal Antibody (Cat. No. bsm-53012M) and with DAPI. Cells were fixed with 4% formaldehyde for 10\u2019 and blocked with PBS\/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K4un antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
To test the specificity an ELISA was performed using a serial dilution of H3K4un (3C5) Monoclonal Antibody (Cat. No. bsm-53012M). The wells were coated with peptides containing the unmodified H3K4 region as well as the mono-, di- and trimethylated H3K4. Figure 2 shows a high specificity of the antibody for the unmodified peptide.
ChIP assays were performed using HeLa cells, the monoclonal antibody against H3K4un (Cat. No. bsm-53012M) and optimized PCR primer sets for qPCR. A titration of the antibody consisting of 1, 5 and 10 \u03bcg per ChIP experiment was analysed. IgG (5 \u03bcg\/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH promoter and for exon 2 of the myoglobin gene. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Catalog #1: bsm-53012M

Applications: WB, ELISA, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-53000M

Applications: WB, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated