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Catalog #1: bsm-60291r-cy7

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-60794r-pe-Cy5.5

Applications: WB, FCM

Reactivity: Human, Mouse

Conjugate: PE-Cy5.5

Catalog #1: bsm-60291r-cy3

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-60291r-pe-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5.5

Catalog #1: bsm-60291r-pe

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-60291r-cy5

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-60291r-percp-cy7

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: PerCP-Cy7

Catalog #1: bsm-60291r-Cy5.5

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-60291r-pe-cy7

Applications: WB

Reactivity: Human

Conjugate: PE-Cy7

Catalog #1: bsm-60794r-apc-cy7

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: APC-Cy7

Catalog #1: bsm-60291r-bf647

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bsm-60291r-apc-cy7

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: APC-Cy7

Catalog #1: bsm-60291r-percp

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: PerCP

Catalog #1: bsm-60291r-percp-Cy5.5

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-60291r-rbitc

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: RBITC

Catalog #1: bsm-60291r-bf680

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-680

Catalog #1: bs-13522r-percp-cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: PerCP-Cy7

Catalog #1: bsm-60291r-hrp

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-60291r-bf350

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bsm-60291r-apc

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: APC

Catalog #1: bsm-60291r-bf405

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bsm-60291r-bf555

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bsm-60291r-bf750

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-750

Catalog #1: bsm-60291r-apc-Cy5.5

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bsm-52170r

Applications: WB, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33294m-percp-cy7

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy7

Catalog #1: bsm-33104m-pe

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bs-7526r-pe

Applications: IF

Reactivity: Rat

Conjugate: PE

Catalog #1: bsm-43637r-Cy5.5

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: Cy5.5

The Hela (treated with 200 ng\/ml PMA for 30 minutes) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-phospho-ERK1 (T202\/Y204)\/ERK2 (T185\/Y187) antibody (bsm-63337R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
The Jurkat (treated with 200 ng\/ml PMA for 30 minutes) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-phospho-ERK1 (T202\/Y204)\/ERK2 (T185\/Y187) antibody (bsm-63337R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
The NIH\/3T3 (treated with 200 ng\/ml PMA for 30 minutes) (M) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-phospho-ERK1 (T202\/Y204)\/ERK2 (T185\/Y187) antibody (bsm-63337R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
The C6 (treated with 200 ng\/ml PMA for 30 minutes) (Rat) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-phospho-ERK1 (T202\/Y204)\/ERK2 (T185\/Y187) antibody (bsm-63337R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bsm-63337r

Applications: FCM, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-43637r-pe

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: PE

The A549 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-SNAI1 antibody (bsm-60947R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
25 ug total protein per lane of various lysates (see on figure) probed with SNAI1 monoclonal antibody, unconjugated (bsm-60947R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-60947r

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-43637r-biotin

Applications: IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-43637r-cy7

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-43637r-percp-Cy5.5

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-43637r-cy5

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-43637r-percp

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: PerCP

Catalog #1: bsm-43637r-hrp

Applications: IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-43637r-rbitc

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: RBITC

Catalog #1: bsm-43637r-cy3

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-43637r-fitc

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-43637r-bf405

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bsm-43637r-bf555

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bsm-43637r-bf594

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bsm-43637r-bf680

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-680

Catalog #1: bsm-43637r-bf750

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-750

Catalog #1: bsm-43637r-bf350

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bsm-43637r-bf647

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-647