SET Polyclonal Antibody

Formalin-fixed and paraffin embedded rat nephroblastoma labeled with Anti SET Polyclonal Antibody, Unconjugated (bs-5943R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (SET) polyclonal Antibody, Unconjugated (bs-5943R) 1:25, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (SET) polyclonal Antibody, Unconjugated (bs-5943R) 1:25, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

$Lane 1: Human HepG2 cell lysates; Lane 2: Human K562 cell lysates; Lane 3: Human MOLT4 cell lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human A431 cell lysates probed with SET Polyclonal Antibody, Unconjugated (bs-5943R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human HepG2 cell lysates; Lane 2: Human K562 cell lysates; Lane 3: Human MOLT4 cell lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human A431 cell lysates probed with SET Polyclonal Antibody, Unconjugated (bs-5943R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
Flow-Cyt
ICC/IF

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Dog
Cow
Horse
Chicken
Rabbit

Overview
Catalog #	bs-5943R
Product Name	SET Polyclonal Antibody
Applications	WB, Flow-Cyt, ICC/IF
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Dog, Cow, Horse, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human SET
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	6418
Swiss Prot	Q01105
Subcellular location	Cytoplasm, Nucleus
Synonyms	SET_HUMAN; Protein SET; HLA-DR-associated protein II; Inhibitor of granzyme A-activated DNase (IGAAD); PHAPII; Phosphatase 2A inhibitor I2PP2A (I-2PP2A); Template-activating factor I (TAF-I); 2PP2A; IGAAD; MRD58; TAF-I; I2PP2A; IPP2A2; PHAPII; TAF-IBETA;
Application Dilution
WB	=1:500-2000
Flow-Cyt	FCMFCM=1:100
ICC/IF	ICCICC=1:25