Nestin Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nestin Monoclonal Antibody, Unconjugated(bsm-55556M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nestin Monoclonal Antibody, Unconjugated(bsm-55556M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Applications

IHC-P
IHC-F
IF

Reactivity

Overview
Catalog #	bsm-55556m
Product Name	Nestin Monoclonal Antibody
Applications	IHC-P, IHC-F, IF
Reactivity	Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant protein corresponding to Mouse Nestin
Clonality	Monoclonal
Clone #	9F5
Isotype	IgG2b,k
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	PBS with 0.02% Proclin300,100 μg/ml BSA and 50% glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	10763
Swiss Prot	Q6P5H2
Subcellular location	Cytoplasm
Synonyms	ESTM 46; FLJ 21841; FLJ21841; Intermediate filament protein; Nbla00170; Nes; NEST_HUMAN; Nestin.
Application Dilution
IHC-P	1:200-400
IHC-F	1:100-500
IF