Phospho-RPS6 (Ser235+Ser236) Recombinant Antibody

$Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser235+Ser236) Monoclonal Antibody, Unconjugated (bsm-60942R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser235+Ser236) Monoclonal Antibody, Unconjugated (bsm-60942R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser235+Ser236) Monoclonal Antibody, Unconjugated (bsm-60942R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser235+Ser236) Monoclonal Antibody, Unconjugated (bsm-60942R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser235+Ser236) Monoclonal Antibody, Unconjugated (bsm-60942R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser235+Ser236) Monoclonal Antibody, Unconjugated (bsm-60942R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser235+Ser236) Monoclonal Antibody, Unconjugated (bsm-60942R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser235+Ser236) Monoclonal Antibody, Unconjugated (bsm-60942R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser235+Ser236) Monoclonal Antibody, Unconjugated (bsm-60942R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser235+Ser236) Monoclonal Antibody, Unconjugated (bsm-60942R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser235+Ser236) Monoclonal Antibody, Unconjugated (bsm-60942R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser235+Ser236) Monoclonal Antibody, Unconjugated (bsm-60942R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$MCF-7 (H) Serum starvation overnight, then grown in 20% FBS media for 30 min, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with Phospho-RPS6 (Ser235+Ser236) monoclonal antibody, unconjugated (bsm-60942R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

MCF-7 (H) Serum starvation overnight, then grown in 20% FBS media for 30 min, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with Phospho-RPS6 (Ser235+Ser236) monoclonal antibody, unconjugated (bsm-60942R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
IHC-P
IHC-F
IF

Reactivity

Human

Overview
Catalog #	bsm-60942r
Product Name	Phospho-RPS6 (Ser235+Ser236) Recombinant Antibody
Applications	WB, IHC-P, IHC-F, IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated Synthesised phosphopeptide derived from human RPS6 around the phosphorylation site of Ser235/236
Modification Site	Ser235+Ser236
Clonality	Recombinant
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	6194
Swiss Prot	P62753
Subcellular location	Cytoplasm, Nucleus, Cell membrane
Synonyms	RPS6(phospho S235+S236); p-RPS6(phospho S235+S236); Air8; DS6; Hemocytes enlarged; Hen; L(1)AIR8; Lethal(1) haemocytes enlarged; Lethal aberrant immune response 8; Lethal(1)abberant immune response[8]; phosphoprotein NP33; Pp30; Ribosomal protein S6; RP S6; RPS 6; RpS6; RS6; RS6_HUMAN; S6; S6 Ribosomal Protein.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IF