CDC7 Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with CDC7 monoclonal antibody, unconjugated (bsm-61146R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with CDC7 monoclonal antibody, unconjugated (bsm-61146R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-61146r
Product Name	CDC7 Recombinant Antibody
Applications	WB, FCM
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CDC7
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	8317
Swiss Prot	O00311
Subcellular location	Nucleus
Synonyms	Cell division cycle 7-related protein kinase, EC 2.7.11.1, CDC7-related kinase, HsCdc7, huCdc7, CDC7, CDC7L1; Hsk1 ; CDC7 kinase.
Background	Kinase involved in initiation of DNA replication. Phosphorylates critical substrates that regulate the G1/S phase transition and initiation of DNA replication, such as MCM proteins and CLASPIN.
Application Dilution
WB	1:500-2000
FCM	FCM1:50-100