Vitamin D3 receptor Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with bsm-61452R monoclonal antibody, unconjugated (bsm-61452R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with bsm-61452R monoclonal antibody, unconjugated (bsm-61452R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Human
Mouse

Predicted Reactivity

Overview
Catalog #	bsm-61452r
Product Name	Vitamin D3 receptor Recombinant Antibody
Applications	WB, IP
Reactivity	Human, Mouse
Predicted Reactivity	Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Vitamin D3 receptor
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	7421
Swiss Prot	P11473
Subcellular location	Cytoplasm, Nucleus
Synonyms	NR1I1; PPP1R163; Protein phosphatase 1, regulatory subunit 163; VDR.
Background	Nuclear receptor for calcitriol, the active form of vitamin D3 which mediates the action of this vitamin on cells. Enters the nucleus upon vitamin D3 binding where it forms heterodimers with the retinoid X receptor/RXR.
Application Dilution
WB	1:500-2000
IP	IP1:20-50