MRP1/ABCC1 Recombinant Antibody

$The HepG2 (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Cells stained with Primary Antibody for 30 min at room temperature,followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-MRP1\/ABCC1 antibody (bsm-61497R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.$

The HepG2 (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Cells stained with Primary Antibody for 30 min at room temperature,followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-MRP1\/ABCC1 antibody (bsm-61497R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

$25 ug total protein per lane of various lysates (see on figure) probed with MRP1\/ABCC1 monoclonal antibody, unconjugated (bsm-61497R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with MRP1\/ABCC1 monoclonal antibody, unconjugated (bsm-61497R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
Flow-Cyt

Reactivity

Human

Overview
Catalog #	bsm-61497r-100l
Product Name	MRP1/ABCC1 Recombinant Antibody
Applications	WB, Flow-Cyt
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ABCC1
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4363
Swiss Prot	P33527
Subcellular location	Cell membrane
Synonyms	ABC29; Abcc1; GSX; Leukotriene C(4) transporter; LTC4 transporter; MRP1.
Background	Mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs. Hydrolyzes ATP with low efficiency.
Application Dilution
WB	1:50-100
Flow-Cyt	1:500-2000