CXCL5 Recombinant Antibody

$A549 (H) cells were treated with or without IL-1 for 24 h, PMA (200 nM) for 24 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with CXCL5 monoclonal antibody, unconjugated (bsm-62331R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

A549 (H) cells were treated with or without IL-1 for 24 h, PMA (200 nM) for 24 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with CXCL5 monoclonal antibody, unconjugated (bsm-62331R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$4% Paraformaldehyde-fixed A549(H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (CXCL5) monoclonal Antibody, unconjugated (bsm-62331R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.$

4% Paraformaldehyde-fixed A549(H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (CXCL5) monoclonal Antibody, unconjugated (bsm-62331R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

Applications

WB
IF(ICC)
IP

Reactivity

Human

Overview
Catalog #	bsm-62331r
Product Name	CXCL5 Recombinant Antibody
Applications	WB, IF(ICC), IP
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CXCL5
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	6374
Swiss Prot	P42830
Subcellular location	Secreted
Synonyms	AMCFII; Cxcl5; ENA78; SCYB5.
Background	Involved in neutrophil activation. In vitro, ENA-78(8-78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes.
Application Dilution
WB	1:500-2000
IF(ICC)	IFICC1:50-200
IP	IP1:20-50