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Catalog #1: bs-2676R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat heart labeled with Anti- ACHE\/Acetylcholinesterase Polyclonal Antibody, Unconjugated (bs-2511R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3) used at 1:200 dilution for 40 minutes at 37\u00b0C
Lane 1: Human K562 cell lysates; Lane 2: Human U-2 OS cell lysates; Lane 3: Human MCF-7 cell lysates; Lane 4: Human SH-SY5Y cell lysates probed with ACHE Polyclonal Antibody, Unconjugated (bs-2511R) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2511r

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-2158R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat ovary tissue labeled with Anti-Wnt3a Polyclonal Antibody (bs-1700R), Unconjugated at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining
L1 rat brain, L2 mouse uterus lysates probed (bs-1700R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted and observed band size: 37kDa.
Lane 1: U2os cell lysates probed with Wnt3a Polyclonal Antibody, Unconjugated (bs-1700R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1700R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded rat adrendal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with \\tRabbit Anti-Integrin alpha L\/CD11a Polyclonal Antibody, Unconjugated (bs-1804R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Human U937 cells probed with Integrin alpha L\/CD11a Antibody, Unconjugated (bs-1804R) at 1:100 for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Catalog #1: bs-1804R

Applications: FCM, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat heart labeled with Anti-GADD45 Polyclonal Antibody, Unconjugated (bs-1360R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Anti-GADD45 Polyclonal Antibody, Unconjugated (bs-1360R) followed by conjugation to the secondary antibody and DAB staining
L1 rat brain lysates L2 rat kidney lysates probed with Rabbit Anti-GADD45 Polyclonal Antibody, Unconjugated (bs-1360R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 18kD. Observed band size: 18kD.
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GADD45 Polyclonal Antibody, Unconjugated (bs-1360R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Catalog #1: bs-1360R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti-FCGBP Polyclonal Antibody, Unconjugated (bs-13168R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Paraformaldehyde-fixed, paraffin embedded Mouse intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FCGBP Polyclonal Antibody, Unconjugated (bs-13168R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FCGBP Polyclonal Antibody, Unconjugated (bs-13168R) at 1:200 overnight at 4\u00b0C, DAB staining.
Positive sample\uff1aParaformaldehyde-fixed, paraffin embedded (rat intestine, left); Negative sample\uff1aParaformaldehyde-fixed, paraffin embedded (rat lung, right);\r\nAntigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (FCGBP) Polyclonal Antibody, Unconjugated (bs-13168R) at 1:200 overnight at 4\u00b0C, followed by secondary antibody and DAB staining.\r\n

Catalog #1: bs-13168R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin-embedded human rectal carcinoma labeled with Rabbit Anti-HLA-E Polyclonal Antibody (bs-1279R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded human rectal carcinoma labeled with Rabbit Anti-HLA-E Polyclonal Antibody (bs-1279R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining
Raji cell lysates probed with Rabbit Anti-HLA E Polyclonal Antibody (bs-1279R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP ) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-1279R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-11847R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human glioma tissue labeled with Anti-Sialoadhesin Polyclonal Antibody, Unconjugated (bs-10751R) at 1:300 followed by conjugation to the secondary antibody (bs-0295G-HRP) and DAB staining.
Formalin-fixed and paraffin embedded rat spleen labeled with Anti-Sialoadhesin Polyclonal Antibody, Unconjugated (bs-10751R) at 1:500 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat spleen labeled with Anti-Sialoadhesin Polyclonal Antibody, Unconjugated (bs-10751R) at 1:500 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat lung labeled with Anti-Sialoadhesin Polyclonal Antibody, Unconjugated (bs-10751R) at 1:500 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-10751R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti FGF8\/HBGF-8 Polyclonal Antibody, Unconjugated (bs-0735R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat testis labeled with Rabbit Anti FGF8\/HBGF-8 Polyclonal Antibody, Unconjugated (bs-0735R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Rat ovarian; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FGF8 Polyclonal Antibody, Unconjugated (bs-0735R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat ovarian; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FGF8 Polyclonal Antibody, Unconjugated (bs-0735R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0735R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse kidney labeled with Rabbit Anti-NFKB Polyclonal Antibody, Unconjugated (bs-10037R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
MCF-7 cells were stained with NFKB Polyclonal Antibody, Unconjugated(bs-10037R) at 1:500 in PBS and incubated for two hours at 37\u00b0C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.

Catalog #1: bs-10037R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human ovary carcinoma labeled Anti-CDKN1B\/P27kip1 Polyclonal Antibody, Unconjugated (bs-0742R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
RSC96 probed with CDKN1B Polyclonal Antibody, Unconjugated (bs-0742R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
MCF-7 cells were stained with CDKN1B Polyclonal Antibody, Unconjugated(bs-0742R) at 1:500 in PBS and incubated for two hours at 37\u00b0C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.
Mouse lung lysates probed with CDKN1B Polyclonal Antibody, unconjugated (bs-0742R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.

Catalog #1: bs-0742R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded human skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Occludin Polyclonal Antibody, Unconjugated (bs-10011R) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: Human A549 cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human Hela cell lysates probed with Occludin Polyclonal Antibody, Unconjugated (bs-10011R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
MCF7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Occludin Antibody(bs-10011R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
MCF7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Occludin Polyclonal Antibody(bs-10011R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-10011R

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-7053R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat liver labeled with \r\nSLCO2A1 Polyclonal Antibody (bs-4710R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC) used at 1:200 dilution for 40 minutes at 37\u00b0C and DAPI staining
Mouse kidney were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with SLCO2A1 Polyclonal Antibody(bs-4710R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-4710R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Mouse

Conjugate: Unconjugated

Lane 1: Rat heart lysates; Lane 2: Mouse heart lysates probed with ETBR\/Endothelin B Receptor Polyclonal Antibody, unconjugated (bs-4198R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Mouse Embryo tissue lysates; Lane 2: Mouse Lung tissue lysates; Lane 3: Mouse Cerebrum tissue lysates; Lane 4: Rat Cerebrum tissue lysates ; Lane 5: Human A431 cell lysates ; Lane 6: Mouse BV-2 cell lysates; Lane 7: Human SH-SY5Y cell lysates probed with Endothelin B Receptor Polyclonal Antibody, Unconjugated (bs-4198R) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-4198R

Applications: WB, ELISA, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-SCD1 Polyclonal Antibody, Unconjugated (bs-3787R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with SCD1 Polyclonal Antibody (bs-3787R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SCD1 Polyclonal Antibody, Unconjugated (bs-3787R) at 1:400 overnight at 4\u00b0C, DAB staining.
A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (SCD1) polyclonal Antibody, Unconjugated (bs-3787R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-3787R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse embryo lysates probed with Phospho-eNOS (Ser1177) Polyclonal Antibody, Unconjugated (bs-3447R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
HepG2 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with eNOS (Ser1177) Polyclonal Antibody(bs-3447R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Human HUVEC cell lysates; Lane 2: Human HUVEC treated with 100 \u00b5M Pervanadate (PV) for 10 minutes lysates probed with Phospho-eNOS (Ser1177) Polyclonal Antibody, Unconjugated (bs-3447R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.

Catalog #1: bs-3447r-TR

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human brain labeled with Anti-HDAC8 Polyclonal Antibody, Unconjugated (bs-2891R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse pancreas labeled with Anti-HDAC8 Polyclonal Antibody, Unconjugated (bs-2891R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Catalog #1: bs-2891R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin-embedded : sheep fat cells labeled with Rabbit Anti-DLK1 Polyclonal Antibody, Unconjugated(bs-2423R) 1:200 followed by conjugation to the secondary PE antibody and DAPI staining
Formalin-fixed and paraffin embedded mouse embryo tissue labeled with Anti-DLK1\/DLL1 Polyclonal Antibody, Unconjugated (bs-2423R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: HepG2 cell lysates probed with DLK1 Polyclonal Antibody, Unconjugated (bs-2423R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2423R

Applications: WB, ELISA, FCM, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

F9 cells probed with Rabbit Anti-SP-C Polyclonal Antibody (bs-10067R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated Secondary Antibody.
Formalin-fixed and paraffin embedded human prostate tissue labeled with Anti-FOXA2\/HNF 3beta Polyclonal Antibody, Unconjugated (bs-2358R) at 1:300 followed by conjugation to the secondary antibody and DAB staining.
Mouse Placenta lysates probed with FOXA2 Polyclonal Antibody, Unconjugated (bs-2358R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with FOXA2 Polyclonal Antibody(bs-2358R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-2358R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human gastric cancer labeled with Anti-VEGFR3 Polyclonal Antibody, Unconjugated (bs-2202R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded Human Gastric Cancer labeled with VEGFR3 Polyclonal Antibody, Unconjugated (bs-2202R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
A549 cells probed with \\tVEGFR3 Polyclonal Antibody, Unconjugated (bs-2202R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Formalin-fixed and paraffin-embedded Human Gastric Cancer labeled with VEGFR3 Polyclonal Antibody, Unconjugated (bs-2202R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-2202R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

A549 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PDEF\/SPDEF Polyclonal Antibody(bs-1866R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded (rat breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (SPDEF) Polyclonal Antibody, Unconjugated (bs-1866R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (SPDEF) Polyclonal Antibody, Unconjugated (bs-1866R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
MCF-7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (SPDEF) polyclonal Antibody, Unconjugated (bs-1866R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-1866R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human laryngocarcinoma labeled with Anti STEAP1 Polyclonal Antibody, Unconjugated (bs-1901R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
A549 lysates probed with STEAP1 Polyclonal Antibody, Unconjugated (bs-1901R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C

Catalog #1: bs-1901R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-1563R

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat heart labeled with Anti-HCN4 Polyclonal Antibody, Unconjugated (bs-1691R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Mouse heart lysates probed with HCN4 Polyclonal Antibody, Unconjugated (bs-1691R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C
Lane 1: Mouse Testis Lysates; Lane 2: Rat Testis Lysates; Lane 3: Human HL-60 cell Lysates; Lane 4: Human MCF-7 cell Lysates; Lane 5: Human A549 cell Lysates. Probed with HCN4 polyclonal Antibody, unconjugated (bs-1691R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
MCF-7 cells were\u00a0 incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with HCN4 Polyclonal Antibody(bs-1691R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-1691R

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human esophageal carcinoma labeled with Anti- Mannan Binding Lectin Polyclonal Antibody, Unconjugated (bs-1582R) at 1:200, followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat intestine tissue labeled with Anti- Mannan Binding Lectin Polyclonal Antibody, Unconjugated (bs-1582R) at 1:200, followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat liver tissue labeled with Anti- Mannan Binding Lectin Polyclonal Antibody, Unconjugated (bs-1582R) at 1:200, followed by conjugation to the secondary antibody and DAB staining.

Catalog #1: bs-1582R

Applications: ELISA, IHC-P, IHC-F, IF

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-13536R-FITC

Applications: IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse

Conjugate: FITC

Catalog #1: bs-1375R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-12313R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse lymphoma tissue labeled with Anti-FRA2\/FOSL2 Polyclonal Antibody, Unconjugated (bs-1188R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human colon carcinoma tissue labeled with Anti-FRA2\/FOSL2 Polyclonal Antibody, Unconjugated (bs-1188R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Small intestine tissue lysates; Lane 2: Rat Small intestine tissue lysates probed with FRA2 Polyclonal Antibody, Unconjugated (bs-1188R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1188R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-beta Defensin 1 Polyclonal Antibody, Unconjugated (bs-1163R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat kidney labeled with Anti-beta Defensin 1 Polyclonal Antibody, Unconjugated (bs-1163R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-beta defensin 1 Polyclonal Antibody, Unconjugated (bs-1163R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Lane 1: Mouse trachea tissue lysates; Lane 2: Mouse Kidney tissue lysates; Lane 3: Rat trachea tissue lysates; Lane 4: Rat Kidney tissue lysates probed with Defensin beta 1 Polyclonal Antibody, Unconjugated (bs-1163R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1163R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat lung tissue labeled with Anti-STAT3 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody (bs-1141R), (SP-0023), and DAB staining
Formalin-fixed and paraffin embedded human cervical tissue labeled with Anti-STAT3 Polyclonal Antibody (bs-1141R), Unconjugated at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining
RSC96 cells probed with Rabbit Anti-STAT3 Polyclonal Antibody (bs-1141R),6ug, for 30 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary (bs-0295G-FITC)at 0.5ug(green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with STAT3 Polyclonal Antibody, Unconjugated (bs-1141R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-1141R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

MCF-7 cell lysates probed with Rabbit Anti-MC4 Receptor Polyclonal Antibody, Unconjugated (bs-11417R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
RSC96 probed with MC4 Receptor Polyclonal Antibody, Unconjugated (bs-11417R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (bs-0295G-FITC) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Embryo lysates probed with MC4 Receptor Polyclonal Antibody, Unconjugated (bs-11417R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-11417R

Applications: ELISA, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-11514R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-11462R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Mouse cells probed with Rabbit Anti-MOT8\/SLC16A2 Polyclonal Antibody (bs-11434R) at 1:100 for 60 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary (bs-0295G-FITC) at 1:100 (green) for 40 minutes compared to control cells (blue).
Rat H9C2 cells probed with Rabbit Anti-MOT8\/SLC16A2 Polyclonal Antibody, Unconjugated (bs-11434R) at 3ug for 30 minutes followed by incubation with a conjugated secondary (bs-0295G-FITC)(green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Mouse heart lysates probed with MOT8 Polyclonal Antibody, Unconjugated (bs-11434R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
U2-OS cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MOT8\/SLC16A2 Polyclonal Antibody(bs-11434R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-11434R

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat spleen labeled with Rabbit Anti-LPA2 Polyclonal Antibody, Unconjugated (bs-10368R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Mouse splenocytes probed with \\tRabbit Anti-LPA2 Polyclonal Antibody, Unconjugated (bs-10368R) at 1:100 for 30 minutes followed by incubation with a FITC conjugated secondary \\n(green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control orange).
Rat testis lysates probed with LPA2 Polyclonal Antibody, Unconjugated (bs-10368R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (LPA2) polyclonal Antibody, Unconjugated (bs-10368R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-10368R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat spleen tissue labeled with Rabbit Anti-MPL Polyclonal Antibody, Unconjugated (bs-10362R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
U2-OS cells(black) were fixed with 4% PFA for 10min at room temperature,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MPL Polyclonal Antibody(bs-10362R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-10362R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse tooth germ labeled with Anti-Oct-3\/Oct-4 Polyclonal Antibody, Unconjugated (bs-1111R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
L1 rat brain lysates L2 rat heart lysates probed with Anti Oct-3\/Oct-4 Polyclonal Antibody, Unconjugated (bs-1111R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 42kD. Observed band size:42kD.\\n
Paraformaldehyde-fixed, paraffin embedded Rat placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Oct-4 Polyclonal Antibody, Unconjugated (bs-1111R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Oct-4 Polyclonal Antibody, Unconjugated (bs-1111R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-1111R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse spleen lysates probed with Rabbit Anti-IL-12 beta Polyclonal Antibody, Unconjugated (bs-10641R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-IL-12 beta Polyclonal Antibody, Unconjugated (bs-10641R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Catalog #1: bs-10641R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Human colon lysates probed with Anti LDL Receptor Polyclonal Antibody, Unconjugated (bs-0705R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT.
HepG2 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with \r\nLDL receptor Polyclonal Antibody(bs-0705R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: K562 cell lysates; Lane 2: Raw264.7 cell lysates; Lane 3: HepG2 cell lysates; Lane 4: SW480 cell lysates probed with LDL receptor Polyclonal Antibody, Unconjugated (bs-0705R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (LDL receptor) polyclonal Antibody, Unconjugated (bs-0705R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-0705R

Applications: WB, ELISA, FCM, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-0819R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti p38MAPK\/MAPK14\/p38Alpha Polyclonal Antibody, Unconjugated (bs-0637R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Independently Validated Antibody, image provided by Science Exchange, badge number 029806: L1 HeLa cell lysate, L2 C6\/36 cell lysates probed with Anti-P38 MAPK Polyclonal Antibody, Unconjugated (bs-0637R) at 1:500 overnight at 4˚C. Followed by conjugation to secondary antibody at 1:100000 for 90 min at 37˚C. Predicted band 41kDa. A strong band was observed in the positive control sample at the correct molecular weight.
MCF-7 cells were stained with p38 MAPK Polyclonal Antibody, Unconjugated(bs-0637R) at 1:500 in PBS and incubated for two hours at 37\u00b0C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.
Paraformaldehyde-fixed, paraffin embedded rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with P38 MAPK Polyclonal Antibody, Unconjugated (bs-0637R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.

Catalog #1: bs-0637R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti GAD65 Polyclonal Antibody, Unconjugated (bs-0400R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat testis labeled with Rabbit Anti GAD65 Polyclonal Antibody, Unconjugated (bs-0400R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
SHSY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (GAD65) polyclonal Antibody, Unconjugated (bs-0400R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Mouse Hippocampus tissue lysates; Lane 4: Rat Cerebrum tissue lysates; Lane 5: Rat Cerebellum tissue lysates; Lane 6: Rat Hippocampus tissue lysates; Lane 7: Human SH-SY5Y cell lysates; Lane 8: Human U87MG cell lysates; Lane 9: Human HeLa cell lysates; Lane 10: Human Jurkat cell lysates probed with GAD65 Polyclonal Antibody, Unconjugated (bs-0400R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0400R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Myelin Protein Zero Polyclonal Antibody, Unconjugated (bs-0337R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Formalin-fixed and paraffin-embedded rat lung labeled with Anti-Myelin Protein Zero Polyclonal Antibody, Unconjugated (bs-0337R)at 1:200 followed by conjugation to the secondary antibody and DAPI staining
Bone lysates probed with Myelin Protein Zero Polyclonal Antibody, Unconjugated (bs-0337R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Spinal cordl lysates; Lane 2: Rat Spinal cord lysates; Lane 3: Mouse Adipose lysates; Lane 4: Rat Adipose lysates probed with Myelin Protein Zero Polyclonal Antibody, Unconjugated (bs-0337R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0337R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat pancreatic tissue labeled with Anti-GIP Polyclonal Antibody, Unconjugated (bs-0098R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded mouse intestine tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GIP Polyclonal Antibody, Unconjugated (bs-0098R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (GIP) polyclonal Antibody, Unconjugated (bs-0098R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-0098R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated