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Lane 1: Mouse liver lysates; Lane 2: Mouse lung lysate; Lane 3: mouse adrenal gland lysate probed with Angiotensin 2 type 1A receptor Polyclonal Antibody, Unconjugated (bs-2132R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC
Mouse kidney lysates probed with Angiotensin 2 type 1A receptor Polyclonal Antibody, Unconjugated (bs-2132R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Lane 1: Mouse Liver lysates; Lane 2: Mouse Heart lysates; Lane 3: Mouse Placenta lysates; Lane 4: Mouse Kidney lysates; Lane 5: Mouse Embryo lysates; Lane 6: Mouse Cerebrum lysates; Lane 7: Mouse NIH\/3T3 cell lysates; Lane 8: Rat Liver lysates; Lane 9: Rat Heart lysates; Lane 10: Rat Kidney lysates; Lane 11: Rat Cerebrum lysates; Lane 12: Rat Embryo lysates probed with Angiotensin 2 type 1A receptor Polyclonal Antibody, Unconjugated (bs-2132R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Angiotensin II type 1A receptor) Polyclonal Antibody, Unconjugated (bs-2132R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-2132r

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded rat spleen tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (BSA or normal goat serum) at 37\u00b0C for 20min; Antibody incubation with TLR4 Polyclonal Antibody, Unconjugated bs-20595R at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Mouse Liver lysates probed with TLR4 Polyclonal Antibody, Unconjugated (bs-20595R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Jurkat cells probed with TLR4 Polyclonal Antibody, unconjugated (bs-20595R) at 1:100 dilution for 30 minutes compared to control cells (dark blue), secondary only (light blue) and isotype control (orange)
Lane 1: Human H9C2 cell lysates; Lane 2: Human K562 cell lysates probed with TLR4 Polyclonal Antibody, Unconjugated (bs-20595R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-20595R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-PAX1 Polyclonal Antibody, Unconjugated (bs-1199R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded mouse tumor tissue labeled with Anti-PAX1 Polyclonal Antibody, Unconjugated (bs-1199R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Catalog #1: bs-1199R

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat myocardium tissue labeled with Anti-Connexin-40 Polyclonal Antibody, Unconjugated (bs-1050R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Mouse spleen cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Connexin-40 Polyclonal Antibody(bs-1050R-AF488)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse Kidney Lysates; Lane 2: Rat Kidney Lysates. Probed with Connexin-40 polyclonal Antibody, unconjugated (bs-1050R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Connexin-40 Polyclonal Antibody, Unconjugated (bs-1050R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bs-1050r-TR

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Collagen III Polyclonal Antibody, Unconjugated (bs-0948R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Hela lysates probed with Collagen III Polyclonal Antibody, Unconjugated (bs-0948R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
25 ug total protein per lane of various lysates (see on figure) probed with Collagen III polyclonal antibody, unconjugated (bs-0948R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed RD (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Collagen III) polyclonal Antibody, unconjugated (bs-0948R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

Catalog #1: bs-0948r

Applications: WB, Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Ovalbumin protein lysates probed with Ovalbumin Polyclonal Antibody, Unconjugated (bs-0283R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Chicken OVA probed with Ovalbumin Polyclonal Antibody, Unconjugated (bs-0283R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0283R

Applications: WB, ELISA

Reactivity: Chicken

Conjugate: Unconjugated

Mouse thymus lysate probed with C5a anaphylatoxin Polyclonal Antibody, Unconjugated (bs-0324R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Mouse thymus lysates probed with C5a anaphylatoxin Polyclonal Antibody, Unconjugated (bs-0324R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human A549 cell lysates; Lane 2: Human Hela cell lysates probed with C5a Polyclonal Antibody, Unconjugated (bs-0324R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.\r\n

Catalog #1: bs-0324r

Applications: WB, ELISA, IHC-P, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Molt-4 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with GCN2 (Thr899) Polyclonal Antibody(bs-3155R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Mouse spleen cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with GCN2 (Thr899) Polyclonal Antibody(bs-3155R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-3155R

Applications: WB, ELISA, FCM

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded mouse tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with IL-22 Polyclonal Antibody (bs-2623R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Lane 1: A549 cell lysates probed with IL-22 Polyclonal Antibody, Unconjugated (bs-2623R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human 293T cell lysates; Lane 2: Human A549 cell lysates probed with IL-22 Polyclonal Antibody, Unconjugated (bs-2623R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2623R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat skin labeled with Anti-Cathepsin L\/CTSL Polyclonal Antibody (bs-1508R), Unconjugated followed by conjugation to the secondary antibody and DAB staining
L1 rat liver, L2 rat brain lysates probed (bs-1508R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band and observed band size: 37kDa.
Image provided by One World Lab validation program. A549 cells probed with Rabbit Anti-Cathepsin L Polyclonal Antibody (bs-1508R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Image provided by One World Lab validation program. A431 cells probed with Rabbit Anti-Cathepsin L Polyclonal Antibody (bs-1508R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.

Catalog #1: bs-1508R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse brain tissue labeled with Anti-CXCL16 Polyclonal Antibody, Unconjugated (bs-1441R) followed by conjugation to the secondary antibody and DAB staining
Images kindly provided by Dr. Denny Cottle from the publication, Fetal inhibition of inflammation improves disease phenotypes in Harlequin Ichthyosis.Formalin-fixed and paraffin embedded mouse skin probed with Rabbit Anti-CXCL16 Polyclonal Antibody (bs-1441R) at 1:50 for two hours at room temperature (green channel).
BV2 (Human) lysates probed with CXCL16 Polyclonal Antibody, Unconjugated (bs-1441R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C

Catalog #1: bs-1441R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin-embedded rat liver labeled with Anti-NGFR\/p75NTR Polyclonal Antibody, Unconjugated(bs-0022R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37\u00b0C
Formalin-fixed and paraffin-embedded rat liver labeled with Anti-NGFR\/p75NTR Polyclonal Antibody, Unconjugated(bs-0022R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37\u00b0C.
Formalin-fixed and paraffin embedded rat brain labeled with Anti-NGFR\/p75NTR Polyclonal Antibody, Unconjugated (bs-0161R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse brain lysates probed with Anti-p75 NGF Receptor Polyclonal Antibody (bs-0161R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.

Catalog #1: bs-0161R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse heart lysate probed with Anti-CD34 Polyclonal Antibody, Unconjugated (bs-8996R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.\\n
Paraformaldehyde-fixed, paraffin embedded mouse kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-CD34 Polyclonal Antibody, Unconjugated (bs-8996R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Human Raji cells probed with CD34 Polyclonal Antibody, Unconjugated (bs-8996R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
Lane 1: Mouse Ovary; Probed with CD34 Polyclonal Antibody, Unconjugated (bs-8996R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bs-8996R

Applications: WB, FCM, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Unconjugated

Catalog #1: bs-7653R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-6970R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse lymphoma labeled with Rabbit Anti BCMA\/CD269 Polyclonal Antibody, Unconjugated (bs-3850R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Raji cells probed with \\tBCMA\/CD269 Polyclonal Antibody, Unconjugated (bs-3850R) at 1:50 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Raji (Positive) and HL-60 (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with BCMA\/CD269 Antibody (bs-3850R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).\r\n

Catalog #1: bs-3850R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Expression of cell surface marker CD34 and CD133 detected by flow cytometry
Formalin-fixed and paraffin embedded human gastric adenocarcinoma tissue labeled with Anti-CD34 Polyclonal Antibody, Unconjugated(bs-2038R) at 1:200 followed by conjugation to the secondary antibody, Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3), and DAPI staining
Lane 1: human colon carcinoma Lane 2: rat kidney lysates probed with Anti CD34 Polyclonal Antibody, Unconjugated (bs-2038R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 39kD. Observed band size: 39kD.
25 ug total protein per lane of various lysates (see on figure) probed with CD34 polyclonal antibody, unconjugated (bs-2038R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bs-2038r

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney labeled with Anti-KIBRA Polyclonal Antibody, Unconjugated (bs-11570R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-KIBRA Polyclonal Antibody, Unconjugated (bs-11570R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Siha cell lysates;Lane 2: A431 cell lysates;Lane 3: MCF-7 cell lysates; Lane 4: U2OS cell lysates probed with KIBRA Polyclonal Antibody, Unconjugated (bs-11570R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with KIBRA Polyclonal Antibody, Unconjugated (bs-11570R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-11570R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-11054R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-VEGFR1\/FLT1 Polyclonal Antibody, Unconjugated (bs-0170R) at 1:400 followed by conjugation to the secondary antibody and DAB staining
Image kindly submitted by Victor Blanco as part of the Free Sample Program. Frozen mouse brain probed with bs-0170R at 1:100, overnight at 4C.
Mouse spleen cells probed with VEGFR1 Polyclonal Antibody, unconjugated (bs-0170R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)

Catalog #1: bs-0170R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-Brdu Polyclonal Antibody (bs-0489R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Image provided by One World Lab validation program. Hela(top) and MCF-7(bottom) cells were labeled with BrdU (10\u03bcM) and stained with Rabbit Anti-BrdU Polyclonal Antibody (bs-0489R) at 1:100 dilution. 2nd antibody without primary antibody was used as control included. Fluorescent signals were detected with primary antibody dilution in both Hela and MCF-7.

Catalog #1: bs-0489R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Species independent

Conjugate: Unconjugated

Formalin-fixed and paraffin-embedded human skin psoriasis labeled with Rabbit Anti- PPARr gamma Polyclonal Antibody, Unconjugated (bs-0530R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse stomach lysates probed with PPAR gamma Polyclonal Antibody, unconjugated (bs-0530R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.
Sheep heart lysates probed with PPAR gamma Polyclonal Antibody, unconjugated (bs-0530R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
293T Cells probed with PPAR gamma Polyclonal Antibody, unconjugated (bs-0530R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:20000 for 60 minutes at 37\u00b0C.

Catalog #1: bs-0530r

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Human breast milk probed with Lactoferrin Polyclonal Antibody, unconjugated (bs-5810R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.\\n
Human Lactoferrin protein (100ng) lysates probed with Lactoferrin Polyclonal Antibody, unconjugated (bs-5810R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Lactoferrin Polyclonal Antibody(bs-5810R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-5810R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat pancreas tissue labeled with Anti-CD105 Polyclonal Antibody (bs-4609R), Unconjugated at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining
L1 rat brain, L2 rat heart lysates probed (bs-4609R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted and observed band size: 70kDa.
Image provided by One World Lab validation program. U138 cells probed with Rabbit Rabbit Anti-CD105 Polyclonal Antibody (bs-4609R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.\\n
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD105) Polyclonal Antibody, Unconjugated (bs-4609R) at 1:200 overnight at 4\u00b0C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining.

Catalog #1: bs-4609R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin-embedded: rat spleen tissue labeled with Rabbit Anti-TNFSF14 Polyclonal Antibody, Unconjugated (bs-2462R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded mouse liver tissue labeled with Rabbit Anti-TNFSF14 Polyclonal Antibody, Unconjugated (bs-2462R) 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 rat brain, L2 rat kidney lysates probed (bs-2462R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted and observed band size: 26kDa.
Lane 1: Mouse Spleen lysates; Lane 2: Mouse Small intestine lysates probed with TNFSF14 Polyclonal Antibody, Unconjugated (bs-2462R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2462R

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-PLAUR Polyclonal Antibody, Unconjugated (bs-1927R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse brain labeled with Anti-PLAUR Polyclonal Antibody, Unconjugated (bs-1927R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.\\n
Formalin-fixed and paraffin embedded mouse adrenal gland labeled with Anti-PLAUR Polyclonal Antibody, Unconjugated (bs-1927R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.\\n
Mouse bone lysates probed with Rabbit Anti-PLAUR Polyclonal Antibody, Unconjugated (bs-1927R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-1927R

Applications: WB, ELISA, FCM, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1053R

Applications: WB, ELISA, IHC-P

Reactivity: Human, Rat, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-LGALS3BP Polyclonal Antibody, Unconjugated (bs-5729R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Heart tissue lysates; Lane 2: Mouse Kidney tissue lysates; Lane 3: Rat Heart tissue lysates; Lane 4: Rat Kidney tissue lysates probed with LGALS3BP Polyclonal Antibody, Unconjugated (bs-5729R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Heart Lysates; Lane 2: Mouse Kidney Lysates; Lane 3: Rat Heart Lysates; Lane 4: Rat Kidney Lysates. Probed with LGALS3BP polyclonal Antibody, unconjugated (bs-5729R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-5729R

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-BRLF1 Polyclonal Antibody, Unconjugated (bs-4542R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-BRLF1 Polyclonal Antibody, Unconjugated (bs-4542R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Catalog #1: bs-4542R

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human, Virus

Conjugate: Unconjugated

L1 rat brain lysates L2 rat kidney lysates probed with Anti Vitamin D Receptor\/VDR Polyclonal Antibody, Unconjugated (bs-2987R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 47kD. Observed band size:47kD.\\n
Formalin-fixed and paraffin embedded rat liver labeled with Anti-Vitamin D Receptor\/VDR Polyclonal Antibody, Unconjugated (bs-2987R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Vitamin D Receptor\/VDR Polyclonal Antibody, Unconjugated (bs-2987R) at 1:400 overnight at 4\u00b0C, DAB staining.
THP-1 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Vitamin D Receptor\/VDR Antibody(bs-2987R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-2987R

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat small intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with VEGF-C Polyclonal Antibody, Unconjugated (bs-1586R) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse Lymph node lysates; Lane 2: Mouse Kidney lysates; Lane 3: Rat Thymus lysates; Lane 4: Rat Lymph node lysates; Lane 5: Rat Kidney lysates probed with VEGF-C Polyclonal Antibody, Unconjugated (bs-1586R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with VEGF-C Polyclonal Antibody(bs-1586R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-1586R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon cancer labeled with Rabbit Anti-Kiss 1\/Kisspeptin Polyclonal Antibody (bs-0749R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human colon cancer Rabbit Anti-Kiss 1\/Kisspeptin Polyclonal Antibody (bs-0749R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin-embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Kisspeptin Polyclonal Antibody, Unconjugated (bs-0749R) at 1:400 overnight at 4\u00b0C, followed by secondary and DAB staining.
Lane 1: Human HeLa cell lysates; Lane 2: Human SW480 cell lysates; Lane 3: Human DU145 cell lysates probed with KISS1 Polyclonal Antibody, Unconjugated (bs-0749R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0749R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat articular cartilage labeled with Anti-MMP-13 Polyclonal Antibody (bs-0575R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Generously provided by Markus Linder from Medical University Vienna as part of the Bioss Discovery Program. Formalin-fixed, paraffin embedded, and decalcified in EDTA mouse bone labeled with Anti-MMP-13 Polyclonal Antibody, Unconjugated (bs-0575R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human bladder cancer labeled with Rabbit Anti-MMP-13 Polyclonal Antibody, Unconjugated (bs-0575R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Human PC3 lysates probed with Rabbit Anti-MMP13 Polyclonal Antibody, Unconjugated (bs-0575R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-0575R

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat liver tissue labeled Anti-Insulin Receptor\/CD220 Polyclonal Antibody, Unconjugated (bs-0681R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Raji cells probed with Insulin Receptor Polyclonal Antibody, unconjugated (bs-0681R) at 1:100 dilution for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange)
Lane 1: Human HepG2 cell lysates; Lane 2: Human 293T cell lysates; Lane 3: Human LOVO cell lysates; Lane 4: Human MOLT-4 cell lysates; Lane 5: Human SW480 cell lysates; Lane 6: Human K562 cell lysates probed with Insulin Receptor Polyclonal Antibody, Unconjugated (bs-0681R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0681R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-8539R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-GnRHR Polyclonal Antibody, Unconjugated (bs-1464R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded rat brain labeled with Rabbit Anti-GnRHR Polyclonal Antibody, Unconjugated(bs-1464R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.
U251 lysates probed with GnRHR Polyclonal Antibody, unconjugated (bs-1464R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.
Lane 1: Mouse Cerebrum lysates; Lane 2: Jurkat cell lysates probed with GnRHR Polyclonal Antibody, Unconjugated (bs-1464R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1464R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat small intestine labeled with Anti-Egr1 Polyclonal Antibody, Unconjugated (bs-1076R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Mouse Lung lysates probed with Egr1 Polyclonal Antibody, Unconjugated (bs-1076R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
K562 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.01%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Egr1 Polyclonal Antibody(bs-1076R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Egr1) polyclonal Antibody, Unconjugated (bs-1076R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-1076R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: human stomach tissue labeled with Anti-GLP-2 Polyclonal Antibody, Unconjugated (bs-0208R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed U2OS cells; Incubated by 0.3% Triton-100 for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GLP-2 Polyclonal Antibody, Unconjugated (bs-0208R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary antibody (bs-0295G-FITC) for 90 minutes and DAPI staining.

Catalog #1: bs-0208R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti- Bax Polyclonal Antibody, Unconjugated (bs-0127M) at 1:200, followed by conjugation to the secondary antibody and DAB staining.
Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Bax) polyclonal Antibody, Unconjugated (bs-0127M) 1:100, 90 minutes at 37\u00b0C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue\/cell: Sh-sy5y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Bax) polyclonal Antibody, Unconjugated (bs-0127M) 1:100, 90 minutes at 37\u00b0C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
25 ug total protein per lane of various lysates (see on figure) probed with Bax monoclonal antibody, unconjugated (bs-0127M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bs-0127m

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-10034R

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: Unconjugated

Mouse lung lysates probed with Anti MHC Class II Polyclonal Antibody, Unconjugated (bs-8481R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 27kD. Observed band size: 30kD
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti-MHC Class II Polyclonal Antibody, Unconjugated (bs-8481R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n

Catalog #1: bs-8481R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Mouse brain lysates probed with Anti SRXN1 Polyclonal Antibody, Unconjugated (bs-8329R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C.
Lane 1: Mouse Cerebrum lysates; Lane 2: Rat Cerebrum lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Human A549 cell lysates probed with SRXN1 Polyclonal Antibody, Unconjugated (bs-8329R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-8329R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney tissue labeled with Rabbit Anti-Urocortin 3 Polyclonal Antibody, Unconjugated (bs-2786R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat kidney tissue labeled with Rabbit Anti-Urocortin 3 Polyclonal Antibody, Unconjugated (bs-2786R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-2786R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

RSC96 cells probed with\\tCD9\/MRP-1 Polyclonal Antibody, Unconjugated (bs-2489R ) at 0.2ug for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Mouse splenocytes probed with\\tCD9\/MRP-1 Polyclonal Antibody, Unconjugated (bs-2489R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded mouse intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD9 Polyclonal Antibody, Unconjugated (bs-2489R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD9 Polyclonal Antibody, Unconjugated (bs-2489R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.

Catalog #1: bs-2489R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with WNT2B Polyclonal Antibody, Unconjugated (bs-1946R) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Heart tissue lysates; Lane 3: Rat Cerebrum tissue lysates; Lane 4: Rat Heart tissue lysates probed with WNT2B Polyclonal Antibody, Unconjugated (bs-1946R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1946R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Antigen: bs-0078P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Formalin-fixed and paraffin embedded human colitis tissue labeled with Anti-TNF-alpha Polyclonal Antibody, Unconjugated(bs-0077R) 1:300 followed by conjugation to the secondary antibody and DAB staining\\n
Formalin-fixed and paraffin embedded human colitis tissue labeled with Anti-TNF-alpha Polyclonal Antibody, Unconjugated(bs-0077R) 1:300 followed by conjugation to the secondary antibody and DAB staining\\n
Human liver lysates probed with Anti TNF alpha Polyclonal Antibody, Unconjugated (bs-0078R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 17kD, 26kD. Observed band size:26kD.

Catalog #1: bs-0078R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: SP2\/0 Cell lysates; Lane 2: Mouse lung lysates; Lane 3: HL-60 Cell lysates; Lane 4: Mouse muscle lysates; probed with GPR84 Polyclonal Antibody, unconjugated (bs-13507R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Mouse Blood cell lysates; Lane 2: Mouse Lung lysates; Lane 3: Rat Bone lysates; Lane 4: Rat Lung lysates ; Lane 5: Rat Cerebrum lysates;Lane 6: Rat Cerebellum lysates; Lane 7: Human U937 cell lysates; Lane 8: Human U87MG cell lysates; Lane 9: Human THP-1 lysates probed with GPR84 Polyclonal Antibody, Unconjugated (bs-13507R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-13507R

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse brain lysates probed with Rabbit Anti-WNT7A Polyclonal Antibody, Unconjugated (bs-6645R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with WNT7A Polyclonal Antibody, Unconjugated (bs-6645R) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse Placenta tissue lysates; Lane 2: Mouse Lung tissue lysates; Lane 3: Mouse Testis tissue lysates; Lane 4: Mouse Kidney tissue lysates ; Lane 5: Rat Placenta tissue lysates; Lane 6: Rat Lung tissue lysates; Lane 7: Rat Testis tissue lysates ; Lane 8: Rat Kidney tissue lysates ; Lane 9: Human Hela cell lysates ; Lane 10: Human A431 cell lysates ; Lane 11: Human SH-SY5Y cell lysates ; Lane 12: Human HepG2 cell lysates probed with WNT7A Polyclonal Antibody, Unconjugated (bs-6645R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-6645R

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated