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Formalin-fixed and paraffin embedded rabbit pancreas labeled with Anti-Bcl-2 Polyclonal Antibody, Unconjugated (bs-4563R) followed by conjugation to the secondary antibody and DAB staining
Mouse splenocytes probed with Rabbit Anti-Bcl-2 Polyclonal Antibody, Unconjugated (bs-4563R) (green) at 1:100 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only(light blue), and isotype control(orange).
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Bcl-2) polyclonal Antibody, Unconjugated (bs-4563R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-4563R

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0468R-BF647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Dog

Conjugate: AbBy Fluor-647

Catalog #1: bs-0036R-PE-Cy7

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: BS-2495R-FITC

Applications: WB, FCM

Reactivity: Human, Pig

Conjugate: PE-Cy5

Catalog #1: bs-0127R-PerCP

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: PerCP

Catalog #1: bs-0756R-BF594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bs-0756R-BF750

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-750

Catalog #1: bs-0756R-PE-Cy5.5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5.5

Antigen: bs-10007P, 2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Rabbit Anti-Goat IgG(bs-0294R-HRP) at 1: 5000; \\nTMB staining\\nRead the data in Microplate Reader by 450nm. \\n
A431 cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with EGFR Antibody(bs-10007R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-10007R

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: Unconjugated

Catalog #1: bs-0468R-BF750

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Dog

Conjugate: AbBy Fluor-750

Catalog #1: bs-0756R-BF488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bs-0756R-APC-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy7

Catalog #1: bs-2495R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Pig

Conjugate: Cy5.5

Catalog #1: bs-2495R-BF350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Pig

Conjugate: AbBy Fluor-350

Catalog #1: bs-2495R-BF405

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Pig

Conjugate: AbBy Fluor-405

Catalog #1: bs-2495R-BF488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Pig

Conjugate: AbBy Fluor-488

Catalog #1: bs-2495R-BF555

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Pig

Conjugate: AbBy Fluor-555

Catalog #1: bs-2495R-BF594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Pig

Conjugate: AbBy Fluor-594

Catalog #1: bs-2495R-BF647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Pig

Conjugate: AbBy Fluor-647

Catalog #1: bs-2495R-BF680

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Pig

Conjugate: AbBy Fluor-680

Catalog #1: bs-2495R-BF750

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Pig

Conjugate: AbBy Fluor-750

Catalog #1: bs-2495R-APC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Pig

Conjugate: APC

Catalog #1: bs-2495R-APC-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Pig

Conjugate: APC-Cy5.5

Catalog #1: bs-2495R-APC-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Pig

Conjugate: APC-Cy7

Catalog #1: bs-2495R-PE-Cy5

Applications: WB, FCM

Reactivity: Human, Pig

Conjugate: PE-Cy5

Catalog #1: bs-2495R-PE-Cy5.5

Applications: WB, FCM

Reactivity: Human, Pig

Conjugate: PE-Cy5.5

Catalog #1: bs-2495R-PE-Cy7

Applications: WB, FCM

Reactivity: Human, Pig

Conjugate: PE-Cy7

Catalog #1: bs-2495R-PerCP

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Pig

Conjugate: PerCP

Catalog #1: bs-2495R-PerCP-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Pig

Conjugate: PerCP-Cy5.5

Catalog #1: bs-2495R-RBITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Pig

Conjugate: RBITC

Catalog #1: bs-10007R-Cy3

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: Cy3

Catalog #1: bs-10007R-Cy5

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: Cy5

Catalog #1: bs-10007R-Cy5.5

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: Cy5.5

Catalog #1: bs-10007R-Cy7

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: Cy7

Catalog #1: bs-10007R-FITC

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: FITC

Catalog #1: bs-10007R-PE

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: PE

Catalog #1: bs-10007R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: Biotin

Catalog #1: bs-10007R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: HRP

Catalog #1: bs-10007R-BF350

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: AbBy Fluor-350

Catalog #1: bs-10007R-BF405

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: AbBy Fluor-405

Catalog #1: bs-10007R-BF488

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: AbBy Fluor-488

Catalog #1: bs-10007R-BF555

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: AbBy Fluor-555

Catalog #1: bs-10007R-BF594

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: AbBy Fluor-594

Catalog #1: bs-10007R-BF647

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: AbBy Fluor-647

Catalog #1: bs-10007R-BF680

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: AbBy Fluor-680

Catalog #1: bs-10007R-BF750

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: AbBy Fluor-750

Catalog #1: bs-10007R-APC

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: APC

Catalog #1: bs-10007R-APC-Cy5.5

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: APC-Cy5.5