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Cancer

Cancer

Our Cancer Research Area is built to support the study of tumor biology, cancer signaling, biomarker discovery, and therapeutic target analysis across a broad range of experimental workflows. This collection includes antibodies for use in WB, IHC, ICC, IF, FC, ELISA, and other common applications.



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Lane 1: full-length PD-L1 transfected 293T; Lane 2: untransfected 293T, probed with PD-L1 (6C8) Monoclonal Antibody, Unconjugated (bsm-58003R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Flow cytometric analysis of 293F cells expressing PD-L1 probed with PD-L1 (6C8) Monoclonal Antibody, unconjugated (bsm-58003R) at 1:100 dilution (red), compared control IgG staining (blue).

Catalog #1: bsm-58003R

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-12260R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bs-2495R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Pig

Conjugate: Cy5.5

Catalog #1: bs-0080R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Cy5.5

Catalog #1: bs-0165R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Cy5.5

Catalog #1: bs-0979R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bs-0980R-Cy5.5

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bs-1497R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bs-1514R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Cy5.5

Catalog #1: bs-2042R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bs-4763R-Cy5.5

Applications: WB, FCM

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bs-4778R-Cy5.5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Cy5.5

Catalog #1: bs-21778R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-21778R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-22022R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bs-22022R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bs-20639R-Cy3

Applications: WB, FCM

Reactivity: Mouse

Conjugate: Cy3

Catalog #1: bs-20639R-Cy5.5

Applications: WB, FCM

Reactivity: Mouse

Conjugate: Cy5.5

Catalog #1: bs-20639R-Cy7

Applications: WB, FCM

Reactivity: Mouse

Conjugate: Cy7

Catalog #1: bs-20639R-FITC

Applications: WB, FCM

Reactivity: Mouse

Conjugate: FITC

Catalog #1: bs-20639R-PE

Applications: WB, FCM

Reactivity: Mouse

Conjugate: PE

Catalog #1: bs-20639R-Cy5

Applications: WB, FCM

Reactivity: Mouse

Conjugate: Cy5

Catalog #1: bs-20782R-Cy3

Applications: WB, FCM

Reactivity: Human, Mouse

Conjugate: Cy3

Catalog #1: bs-20782R-Cy5.5

Applications: WB, FCM

Reactivity: Human, Mouse

Conjugate: Cy5.5

Catalog #1: bs-20782R-Cy7

Applications: WB, FCM

Reactivity: Human, Mouse

Conjugate: Cy7

Catalog #1: bs-21778R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-21778R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-21778R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bs-21778R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-21778R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-22022R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy3

Catalog #1: bs-22022R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy5

Catalog #1: bs-22022R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy5.5

Catalog #1: bs-22022R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy7

Catalog #1: bs-22022R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: FITC

Catalog #1: bsm-51506m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PSMA5 Monoclonal Antibody, Unconjugated (bsm-51520M) at 1:25 overnight at 4\u00b0C, DAB staining.
Lane 1: K562 cell lysates probed with PSMA5 Monoclonal Antibody, Unconjugated (bsm-51520M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human liver tissue lysates; Lane 2: Hela cell lysates; Lane 3: HepG2 cell lysates; Lane 4: MCF-7 cell lysates;Lane 5: Neuro-2a cell lysates; Lane 6: PC-12 cell lysates probed with PSMA5 Monoclonal Antibody, Unconjugated (bsm-51520M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51520m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-24719R-HRP

Applications: WB, ELISA

Reactivity:

Conjugate: HRP

Catalog #1: bs-24719R-Biotin

Applications: WB, ELISA

Reactivity:

Conjugate: Biotin

Catalog #1: bs-24719R

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Tissue:Human breast cancer\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60020R
25 ug total protein per lane of various lysates (see on figure) probed with ERBB2\/HER2 monoclonal antibody, unconjugated (bsm-60020R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-60020r-100ul

Applications: WB

Reactivity:

Conjugate: Unconjugated

Cell line: Daudi<br>\r\nFixative: 4% Paraformaldehyde\t<br>\r\nPermeabilization: 0.1% TritonX-100\t<br>\r\nPrimary ab dilution: 1:100\t<br>\r\nPrimary incubation condition: 1 hour at room temperature\t<br>\r\nSecondary ab: Goat Anti-Rabbit IgG\t<br>\r\nNuclear counter stain: DAPI (Blue)\t<br>\r\nComment: Color green is the positive signal for bsm-60233R\t<br>
\tParaformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD22 Monoclonal Antibody, Unconjugated(bsm-60233R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD22 Monoclonal Antibody, Unconjugated(bsm-60233R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with CD22 monoclonal antibody, unconjugated (bsm-60233R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-60233r-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

4% Paraformaldehyde-fixed HepG2 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (PSMA1) monoclonal Antibody, unconjugated (bsm-60572R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
25 ug total protein per lane of various lysates (see on figure) probed with PSMA1 monoclonal antibody, unconjugated (bsm-60572R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PSMA1 Monoclonal Antibody, Unconjugated(bsm-60572R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PSMA1 Monoclonal Antibody, Unconjugated(bsm-60572R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-60572r-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-60492m

Applications: WB, ICC/IF

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-60283M

Applications: FCM

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-0979R-BF350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-350

Catalog #1: bs-0979R-BF405

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bs-0979R-BF488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-488