CD144/VE Cadherin Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-0878R-BF750-100uL |
| Product Name | CD144/VE Cadherin Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse vascular endothelial cadherin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 12562 |
| Swiss Prot | P55284 |
| Subcellular location | Cell membrane |
| Synonyms | 7B4; Vec; VECD; Cd144; VEcad; VE-Cad; AA48225; Cadherin-5; Vascular endothelial cadherin; VE-cadherin; Cdh5 |
| Background | Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CD144/VE Cadherin Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-0878R-BF750) validated for, and at what dilution?
bs-0878R-BF750 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD144/VE Cadherin Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-0878R-BF750) react with?
Tested reactivity: Human.
How should bs-0878R-BF750 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CD144/VE Cadherin Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-0878R-BF750)?
KLH conjugated synthetic peptide derived from mouse vascular endothelial cadherin
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-0878R-BF750)?
AbBy Fluor-750 on bs-0878R-BF750: excitation / emission 749nm/775nm.