IGFBP3 Polyclonal Antibody, AbBy Fluor-405 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1434R-BF405-100uL |
| Product Name | IGFBP3 Polyclonal Antibody, AbBy Fluor-405 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse |
| Specifications | |
| Conjugation | AbBy Fluor-405 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human IGFBP3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3486 |
| Swiss Prot | P17936 |
| Subcellular location | Secreted |
| Synonyms | IBP3; BP-53; Insulin-like growth factor-binding protein 3; IBP-3; IGF-binding protein 3; IGFBP-3; IGFBP3 |
| Background | IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is IGFBP3 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-1434R-BF405) validated for, and at what dilution?
bs-1434R-BF405 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does IGFBP3 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-1434R-BF405) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse.
How should bs-1434R-BF405 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise IGFBP3 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-1434R-BF405)?
KLH conjugated synthetic peptide derived from human IGFBP3
What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bs-1434R-BF405)?
AbBy Fluor-405 on bs-1434R-BF405: excitation / emission 401nm/421nm.