phospho-GSK-3 Beta (Ser9) Polyclonal Antibody, PE Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-2066R-PE-100uL |
| Product Name | phospho-GSK-3 Beta (Ser9) Polyclonal Antibody, PE Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human GSK-3 Beta around the phosphorylation site of Ser9 |
| Modification Site | Ser9 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2932 |
| Swiss Prot | P49841 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B; GSK3B |
| Background | The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009] |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | |
| IF(IHC-F) | |
| IF(ICC) | |
Frequently asked questions
Which applications is phospho-GSK-3 Beta (Ser9) Polyclonal Antibody, PE Conjugated (bs-2066R-PE) validated for, and at what dilution?
bs-2066R-PE is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-GSK-3 Beta (Ser9) Polyclonal Antibody, PE Conjugated (bs-2066R-PE) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-2066R-PE be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-GSK-3 Beta (Ser9) Polyclonal Antibody, PE Conjugated (bs-2066R-PE)?
KLH conjugated synthetic phosphopeptide derived from human GSK-3 Beta around the phosphorylation site of Ser9
What are the excitation and emission wavelengths of the PE conjugate (bs-2066R-PE)?
PE on bs-2066R-PE: excitation / emission 496,564nm/578nm.