IL-1 Beta Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-6319R-Cy5.5-100uL |
| Product Name | IL-1 Beta Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat, Bovine, Rabbit |
| Predicted Reactivity | Dog, Horse |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human IL-1 Beta |
| Immunogen Range | 161-269/269 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles |
| Target | |
| Gene ID | 3553 |
| Swiss Prot | P01584 |
| Subcellular location | Secreted |
| Synonyms | IL-1; IL1F2; IL1-BETA; Interleukin-1 beta; IL-1 beta; Catabolin; IL1B |
| Background | Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is IL-1 Beta Polyclonal Antibody, Cy5.5 Conjugated (BS-6319R-CY5-6) validated for, and at what dilution?
BS-6319R-CY5-6 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does IL-1 Beta Polyclonal Antibody, Cy5.5 Conjugated (BS-6319R-CY5-6) react with?
Tested reactivity: Human, Mouse, Rat, Bovine, Rabbit. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Horse.
How should BS-6319R-CY5-6 be stored?
Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
What immunogen was used to raise IL-1 Beta Polyclonal Antibody, Cy5.5 Conjugated (BS-6319R-CY5-6)?
KLH conjugated synthetic peptide derived from human IL-1 Beta Immunogen range: 161-269/269.
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-6319R-CY5-6)?
Cy5.5 on BS-6319R-CY5-6: excitation / emission 675nm/694nm.