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Catalog #1: bsm-33052m-rbitc-100ul

Applications: WB, FCM

Reactivity: Human

Conjugate: RBITC

Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (human CD44) Monoclonal Antibody, Unconjugated (bsm-30038M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (human CD44) polyclonal Antibody, Unconjugated (bsm-30038M) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.PBS instead of the primary antibody was used as the blank control.
Hela cells were\u00a0incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with CD44 .Antibody(bsm-30038M)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (human CD44) Monoclonal Antibody, Unconjugated (bsm-30038M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-30038m

Applications: IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-30038m-cy3-100ul

Applications: IF, Flow-Cyt

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-30038m-cy5-100ul

Applications: IF, Flow-Cyt

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-30038m-Cy5.5-100ul

Applications: IF, Flow-Cyt

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-30038m-cy7-100ul

Applications: IF, Flow-Cyt

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-30038m-bf350-100ul

Applications: IF, Flow-Cyt

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bsm-30038m-bf405-100ul

Applications: IF, Flow-Cyt

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bsm-30038m-bf488-100ul

Applications: IF, Flow-Cyt

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bsm-30038m-bf555-100ul

Applications: IF, Flow-Cyt

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bsm-30038m-bf594-100ul

Applications: IF, Flow-Cyt

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bsm-30038m-bf647-100ul

Applications: IF

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bsm-30038m-bf680-100ul

Applications: IF

Reactivity: Human

Conjugate: AbBy Fluor-680

Catalog #1: bsm-30038m-bf750-100ul

Applications: IF, Flow-Cyt

Reactivity: Human

Conjugate: AbBy Fluor-750

Catalog #1: bsm-30038m-apc-Cy5.5-100ul

Applications: IF, Flow-Cyt

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bsm-30038m-apc-cy7-100ul

Applications: Flow-Cyt

Reactivity: Human

Conjugate: APC-Cy7

Catalog #1: bsm-30038m-pe-Cy5.5-100ul

Applications: Flow-Cyt

Reactivity: Human

Conjugate: PE-Cy5.5

Catalog #1: bsm-30038m-percp-Cy5.5-100ul

Applications: IF, Flow-Cyt

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-30038m-percp-100ul

Applications: IF, Flow-Cyt

Reactivity: Human

Conjugate: PerCP

Catalog #1: bsm-30038m-rbitc-100ul

Applications: IF, Flow-Cyt

Reactivity: Human

Conjugate: RBITC